FastQCFastQC Report
Thu 2 Feb 2017
SRR4062383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699764
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19900.2843815915079941No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16960.24236742673244124No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8680.12404181981353712No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8310.11875432288600157No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7880.11260939402427105No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7580.10832223435329624No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7350.10503541193888224No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7290.1041779800046873No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7170.10246311613629738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA2550.025.01952636
CGGTCCA2700.024.44436310
GTTAAGC551.5927179E-423.9999243
CGTTCTA852.3497887E-723.29404312
GCCGGTC2750.023.1999248
CTAGCGG2850.022.38589329
GCAATAC2950.022.37280737
ACCGCGC500.00257941121.999938
TAGCGGC2900.021.9999330
CGTCTTA500.00257941121.9999315
TCTTGCG2700.021.9999282
ATACGAA3050.021.63927540
CAATACG2950.021.62704738
TAGGACG2750.021.599934
CGAATGC3000.021.26659843
AATACGA3050.020.91796539
TACTCCG956.8580266E-720.84203517
GTGCGAT856.1075425E-620.70581640
CCGGTCC3300.020.66669
GGTCCAA3350.020.35814311
Produced by FastQC (version 0.11.5)