##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	699764
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221806208950447	32.0	32.0	32.0	32.0	32.0
2	31.40178545909764	32.0	32.0	32.0	32.0	32.0
3	31.506705117725403	32.0	32.0	32.0	32.0	32.0
4	31.61183627623027	32.0	32.0	32.0	32.0	32.0
5	31.542081330277064	32.0	32.0	32.0	32.0	32.0
6	35.15272434706558	36.0	36.0	36.0	36.0	36.0
7	35.16896267884601	36.0	36.0	36.0	36.0	36.0
8	35.1115175973614	36.0	36.0	36.0	36.0	36.0
9	35.22476149101697	36.0	36.0	36.0	36.0	36.0
10	35.11520741278488	36.0	36.0	36.0	36.0	36.0
11	35.24846233873134	36.0	36.0	36.0	36.0	36.0
12	35.15254285730618	36.0	36.0	36.0	36.0	36.0
13	35.18918378196078	36.0	36.0	36.0	36.0	36.0
14	35.15007774049537	36.0	36.0	36.0	36.0	36.0
15	35.11675364837288	36.0	36.0	36.0	36.0	36.0
16	35.1162534797446	36.0	36.0	36.0	36.0	36.0
17	35.09153371708176	36.0	36.0	36.0	36.0	36.0
18	35.1116204891935	36.0	36.0	36.0	36.0	36.0
19	35.085101548522076	36.0	36.0	36.0	36.0	36.0
20	35.073311859426894	36.0	36.0	36.0	36.0	36.0
21	35.04431779857209	36.0	36.0	36.0	36.0	36.0
22	35.025277093420065	36.0	36.0	36.0	36.0	36.0
23	34.976773597955884	36.0	36.0	36.0	36.0	36.0
24	34.94547161614487	36.0	36.0	36.0	32.0	36.0
25	34.910354062226695	36.0	36.0	36.0	32.0	36.0
26	34.861060300329825	36.0	36.0	36.0	32.0	36.0
27	34.822180049273754	36.0	36.0	36.0	32.0	36.0
28	34.79641136154475	36.0	36.0	36.0	32.0	36.0
29	34.76189115187406	36.0	36.0	36.0	32.0	36.0
30	34.748902487124234	36.0	36.0	36.0	32.0	36.0
31	34.732748469484	36.0	36.0	36.0	32.0	36.0
32	34.70043471799064	36.0	36.0	36.0	32.0	36.0
33	34.65491508565745	36.0	36.0	36.0	32.0	36.0
34	34.6461049725336	36.0	36.0	36.0	32.0	36.0
35	34.574596578274964	36.0	36.0	36.0	32.0	36.0
36	34.556684825169626	36.0	36.0	36.0	32.0	36.0
37	34.4976020486907	36.0	36.0	36.0	32.0	36.0
38	34.44162460486678	36.0	36.0	36.0	32.0	36.0
39	34.35429373331581	36.0	36.0	36.0	32.0	36.0
40	34.309324286473725	36.0	36.0	36.0	32.0	36.0
41	34.29654855065422	36.0	36.0	36.0	32.0	36.0
42	34.17511761108031	36.0	36.0	36.0	32.0	36.0
43	34.19858695217245	36.0	36.0	36.0	32.0	36.0
44	34.16878118908661	36.0	36.0	36.0	32.0	36.0
45	34.03640798897914	36.0	36.0	36.0	32.0	36.0
46	34.1014970761571	36.0	36.0	36.0	32.0	36.0
47	34.044520724129846	36.0	36.0	36.0	32.0	36.0
48	34.02310064536044	36.0	36.0	36.0	32.0	36.0
49	34.03889597064153	36.0	36.0	36.0	32.0	36.0
50	33.496778914033875	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	10.0
22	62.0
23	183.0
24	549.0
25	1273.0
26	2807.0
27	5413.0
28	8977.0
29	13156.0
30	17888.0
31	25566.0
32	37471.0
33	55558.0
34	118816.0
35	412032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28707737339782	17.875518814530235	11.262991801872877	25.574412010199065
2	15.785329240337727	20.37680140396609	36.91113169041862	26.92673766527756
3	19.312137555340243	24.30569250688091	28.299621871436287	28.082548066342554
4	12.427350402922134	15.318043394692202	35.72480968556497	36.529796516820696
5	13.709336290520804	37.33244350952607	33.77238611874861	15.185834081204522
6	34.62234855142985	35.06035649416442	16.604288047742152	13.71300690666358
7	29.697483290771302	30.3537054688516	21.0782793602977	18.870531880079398
8	28.675953607216147	32.98369164461161	19.580744365243138	18.7596103829291
9	27.262334158373395	13.913690901503935	18.521530115867634	40.30244482425503
10	15.744022270365438	27.079844061712233	31.632950537609823	25.543183130312507
11	37.812462487352875	21.18485660879954	21.227013678897457	19.775667224950126
12	23.978369850406708	23.866617888316632	29.374189012295577	22.780823248981086
13	30.337656695685972	19.511721094540448	25.320393732744183	24.830228477029397
14	23.415037069640622	19.723649687608965	24.878244665344315	31.983068577406097
15	25.638358075008146	27.196740615407478	22.96102686048439	24.20387444909998
16	26.238989144911713	25.61363545423886	24.472250644503006	23.675124756346424
17	23.699418661148616	25.745822877427248	26.204548962221548	24.350209499202588
18	25.20478332695023	24.414802704911942	27.1933108876707	23.18710308046713
19	25.31096198146804	26.07064667516477	24.845090630555443	23.77330071281175
20	24.873528789706246	24.173578520758426	26.773597955882266	24.17929473365306
21	25.997193339468733	24.882674730337655	24.1385667167788	24.981565213414807
22	24.667888030821818	25.152337073641974	25.251513367363852	24.928261528172353
23	23.596098113078124	24.692610651591107	25.50088315489222	26.210408080438548
24	23.99851950086029	26.294293504667287	25.168342469746946	24.538844524725477
25	25.10717899177437	24.670317421302038	25.079026643268303	25.14347694365529
26	23.42203943043655	25.31853596355343	26.164106755992023	25.095317850018006
27	25.050302673472768	25.360407223006614	25.49859666973437	24.09069343378625
28	24.05125156481328	24.63873534505919	25.80912993523531	25.50088315489222
29	23.781732126831333	25.392846731183656	26.15681858455136	24.66860255743365
30	24.643879936664362	25.123184387879345	26.443486661217207	23.789449014239086
31	24.200301816040838	25.231935338199733	25.05330368524245	25.51445916051697
32	24.06911473010901	25.468300741392813	25.178917463602012	25.283667064896164
33	23.563086983611615	24.792644377247186	26.158390543097386	25.485878096043805
34	24.18072378687672	25.785550557044946	26.550236937024486	23.483488719053852
35	25.33925723529647	25.10003372565608	26.03563487118514	23.525074167862307
36	24.062112369313084	25.88429813479973	25.556616230614893	24.496973265272292
37	25.264946467666245	25.30438833663921	25.236794119160173	24.193871076534375
38	24.39336690655707	25.412138949703046	24.96713177585586	25.227362367884034
39	24.719905568162982	24.966845965211128	25.614778696817787	24.698469769808106
40	25.324966703059886	25.446722037715574	25.22564750401564	24.0026637552089
41	23.442046175567764	25.57819493429213	26.589821711319818	24.389937178820286
42	25.854859638392373	25.6293550396991	25.504169977306635	23.011615344601893
43	24.718476514939322	24.32034228682813	25.327396093540106	25.633785104692443
44	24.15357177562721	25.236651213837806	26.17482465516946	24.434952355365525
45	24.513550282666728	25.494595320708125	25.55432974545704	24.437524651168108
46	24.121846794061998	24.696612000617353	26.30644045706838	24.87510074825227
47	24.58571747046147	24.4212334444184	26.818898943072234	24.17415014204789
48	24.684465048216257	26.359029615699008	24.59943638140859	24.357068954676148
49	23.630967011735386	25.520461184056337	26.382037372599903	24.466534431608373
50	23.337582384918342	27.068411635922967	24.509691838962848	25.084314140195836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	7.5
2	10.0
3	52.5
4	95.0
5	111.5
6	128.0
7	118.5
8	109.0
9	141.0
10	173.0
11	284.5
12	396.0
13	671.0
14	946.0
15	1306.5
16	1667.0
17	2005.0
18	2343.0
19	2598.0
20	2853.0
21	3243.0
22	3633.0
23	3849.0
24	4065.0
25	4373.5
26	4682.0
27	5734.0
28	6786.0
29	7891.0
30	8996.0
31	10211.5
32	11427.0
33	13255.0
34	15083.0
35	17359.5
36	19636.0
37	23289.5
38	26943.0
39	28957.0
40	30971.0
41	33904.5
42	36838.0
43	38660.5
44	40483.0
45	45806.0
46	51129.0
47	55484.5
48	59840.0
49	59792.0
50	59744.0
51	54605.0
52	49466.0
53	47754.5
54	46043.0
55	45602.0
56	45161.0
57	43507.5
58	41854.0
59	38169.0
60	34484.0
61	30335.5
62	26187.0
63	22835.5
64	19484.0
65	16931.5
66	14379.0
67	12413.0
68	10447.0
69	9670.5
70	8894.0
71	6893.5
72	4893.0
73	4395.5
74	3898.0
75	2789.0
76	1680.0
77	1546.5
78	1413.0
79	1182.5
80	952.0
81	785.0
82	618.0
83	527.5
84	437.0
85	368.5
86	300.0
87	218.0
88	136.0
89	86.5
90	37.0
91	21.5
92	6.0
93	7.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01314728965765601
2	0.004572970315706438
3	2.858106447316524E-4
4	1.429053223658262E-4
5	0.0
6	7.14526611829131E-4
7	1.429053223658262E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	699764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.851874326766215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96740014334732	46.841158576579225
2	11.989763591823538	13.872605930436249
3	3.247225008704525	5.635741593429196
4	1.2433406173198769	2.877183405542138
5	0.6443141685947138	1.8637391154248122
6	0.375351897497387	1.3028886481399242
7	0.24045660173810762	0.9737605573355994
8	0.1699921802791701	0.786749300003483
9	0.13550110472028792	0.7055093593274477
>10	0.7860242923900375	8.838581428448833
>50	0.10688724336321369	4.306299227164799
>100	0.08878319518366687	9.813870601333054
>500	0.0044639595343324125	1.6530768689673323
>1k	4.959955038147124E-4	0.528835387867869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1990	0.2843815915079941	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1696	0.24236742673244124	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	868	0.12404181981353712	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	831	0.11875432288600157	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	788	0.11260939402427105	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	758	0.10832223435329624	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	735	0.10503541193888224	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	729	0.1041779800046873	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	717	0.10246311613629738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.429053223658262E-4
2	0.0	0.0	0.0	0.0	1.429053223658262E-4
3	0.0	0.0	0.0	0.0	1.429053223658262E-4
4	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
5	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
6	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
7	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
8	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
9	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
10	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
11	0.0	0.0	0.0	2.858106447316524E-4	1.429053223658262E-4
12	0.0	0.0	0.0	2.858106447316524E-4	2.858106447316524E-4
13	0.0	0.0	0.0	2.858106447316524E-4	4.287159670974786E-4
14	0.0	0.0	0.0	2.858106447316524E-4	4.287159670974786E-4
15	0.0	0.0	0.0	4.287159670974786E-4	5.716212894633048E-4
16	0.0	0.0	0.0	4.287159670974786E-4	5.716212894633048E-4
17	0.0	0.0	0.0	5.716212894633048E-4	5.716212894633048E-4
18	0.0	0.0	0.0	7.145266118291309E-4	5.716212894633048E-4
19	0.0	0.0	0.0	0.0010003372565607833	5.716212894633048E-4
20	0.0	0.0	0.0	0.0010003372565607833	7.145266118291309E-4
21	0.0	0.0	0.0	0.0011432425789266096	7.145266118291309E-4
22	0.0	0.0	0.0	0.0014290532236582618	7.145266118291309E-4
23	0.0	0.0	0.0	0.003572633059145655	7.145266118291309E-4
24	0.0	0.0	0.0	0.008002698052486266	7.145266118291309E-4
25	0.0	0.0	0.0	0.01128952046690027	7.145266118291309E-4
26	0.0	0.0	0.0	0.014004721591850967	0.0010003372565607833
27	0.0	0.0	0.0	0.017863165295728275	0.0010003372565607833
28	0.0	0.0	0.0	0.02586586334821454	0.0010003372565607833
29	0.0	0.0	0.0	0.03944186897296803	0.0010003372565607833
30	0.0	0.0	0.0	0.06202090990676857	0.0010003372565607833
31	0.0	0.0	0.0	0.10232021081393156	0.0010003372565607833
32	0.0	0.0	0.0	0.1456205234907769	0.0010003372565607833
33	0.0	0.0	0.0	0.19277927987149954	0.0010003372565607833
34	0.0	0.0	0.0	0.2545143791335365	0.0010003372565607833
35	0.0	0.0	0.0	0.3122481293693302	0.0010003372565607833
36	0.0	0.0	0.0	0.40828050599916543	0.0010003372565607833
37	0.0	0.0	0.0	0.5620466328647944	0.0010003372565607833
38	0.0	0.0	0.0	0.7336759250261516	0.0010003372565607833
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	255	0.0	25.019526	36
CGGTCCA	270	0.0	24.444363	10
GTTAAGC	55	1.5927179E-4	23.999924	3
CGTTCTA	85	2.3497887E-7	23.294043	12
GCCGGTC	275	0.0	23.199924	8
CTAGCGG	285	0.0	22.385893	29
GCAATAC	295	0.0	22.372807	37
ACCGCGC	50	0.002579411	21.99993	8
TAGCGGC	290	0.0	21.99993	30
CGTCTTA	50	0.002579411	21.99993	15
TCTTGCG	270	0.0	21.999928	2
ATACGAA	305	0.0	21.639275	40
CAATACG	295	0.0	21.627047	38
TAGGACG	275	0.0	21.59993	4
CGAATGC	300	0.0	21.266598	43
AATACGA	305	0.0	20.917965	39
TACTCCG	95	6.8580266E-7	20.842035	17
GTGCGAT	85	6.1075425E-6	20.705816	40
CCGGTCC	330	0.0	20.6666	9
GGTCCAA	335	0.0	20.358143	11
>>END_MODULE