##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209923664122137	32.0	32.0	32.0	32.0	32.0
2	31.431297709923665	32.0	32.0	32.0	32.0	32.0
3	31.464885496183207	32.0	32.0	32.0	32.0	32.0
4	31.608396946564884	32.0	32.0	32.0	32.0	32.0
5	31.568702290076335	32.0	32.0	32.0	32.0	32.0
6	35.261068702290075	36.0	36.0	36.0	36.0	36.0
7	35.19770992366412	36.0	36.0	36.0	36.0	36.0
8	35.10381679389313	36.0	36.0	36.0	36.0	36.0
9	35.1793893129771	36.0	36.0	36.0	36.0	36.0
10	35.24351145038168	36.0	36.0	36.0	36.0	36.0
11	35.19847328244275	36.0	36.0	36.0	36.0	36.0
12	35.135114503816794	36.0	36.0	36.0	36.0	36.0
13	35.18854961832061	36.0	36.0	36.0	36.0	36.0
14	35.18625954198473	36.0	36.0	36.0	36.0	36.0
15	35.26946564885496	36.0	36.0	36.0	36.0	36.0
16	35.27786259541985	36.0	36.0	36.0	36.0	36.0
17	35.07175572519084	36.0	36.0	36.0	36.0	36.0
18	35.055725190839695	36.0	36.0	36.0	36.0	36.0
19	35.21221374045802	36.0	36.0	36.0	36.0	36.0
20	35.23435114503817	36.0	36.0	36.0	36.0	36.0
21	35.1824427480916	36.0	36.0	36.0	36.0	36.0
22	34.98549618320611	36.0	36.0	36.0	36.0	36.0
23	35.20458015267175	36.0	36.0	36.0	36.0	36.0
24	35.1648854961832	36.0	36.0	36.0	36.0	36.0
25	35.152671755725194	36.0	36.0	36.0	36.0	36.0
26	34.94580152671756	36.0	36.0	36.0	32.0	36.0
27	34.93511450381679	36.0	36.0	36.0	36.0	36.0
28	35.033587786259545	36.0	36.0	36.0	36.0	36.0
29	34.936641221374046	36.0	36.0	36.0	32.0	36.0
30	34.929770992366414	36.0	36.0	36.0	36.0	36.0
31	34.82671755725191	36.0	36.0	36.0	32.0	36.0
32	34.91679389312977	36.0	36.0	36.0	36.0	36.0
33	35.055725190839695	36.0	36.0	36.0	36.0	36.0
34	34.94351145038168	36.0	36.0	36.0	32.0	36.0
35	34.850381679389315	36.0	36.0	36.0	32.0	36.0
36	34.66564885496183	36.0	36.0	36.0	32.0	36.0
37	34.64427480916031	36.0	36.0	36.0	32.0	36.0
38	34.39541984732824	36.0	36.0	36.0	32.0	36.0
39	34.60152671755725	36.0	36.0	36.0	32.0	36.0
40	34.2587786259542	36.0	36.0	36.0	32.0	36.0
41	34.310687022900765	36.0	36.0	36.0	32.0	36.0
42	34.22977099236641	36.0	36.0	36.0	32.0	36.0
43	34.33969465648855	36.0	36.0	36.0	32.0	36.0
44	34.32595419847328	36.0	36.0	36.0	32.0	36.0
45	34.35190839694656	36.0	36.0	36.0	32.0	36.0
46	34.131297709923665	36.0	36.0	36.0	32.0	36.0
47	34.141984732824426	36.0	36.0	36.0	32.0	36.0
48	34.29160305343512	36.0	36.0	36.0	32.0	36.0
49	34.32748091603053	36.0	36.0	36.0	32.0	36.0
50	33.47022900763359	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	0.0
25	2.0
26	6.0
27	10.0
28	11.0
29	26.0
30	31.0
31	38.0
32	69.0
33	85.0
34	199.0
35	831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.465648854961835	20.916030534351147	11.374045801526718	28.24427480916031
2	15.725190839694655	23.969465648854964	33.05343511450381	27.25190839694657
3	19.236641221374047	24.427480916030532	31.832061068702288	24.50381679389313
4	13.435114503816795	20.076335877862594	33.05343511450381	33.43511450381679
5	15.34351145038168	34.50381679389313	31.603053435114504	18.549618320610687
6	33.3587786259542	36.25954198473282	16.106870229007633	14.274809160305344
7	25.64885496183206	29.465648854961835	23.893129770992367	20.99236641221374
8	26.717557251908396	33.74045801526718	21.14503816793893	18.396946564885496
9	22.519083969465647	19.84732824427481	22.137404580152673	35.49618320610687
10	16.030534351145036	28.24427480916031	31.14503816793893	24.580152671755727
11	28.931297709923665	23.740458015267176	24.198473282442748	23.129770992366414
12	21.14503816793893	28.549618320610687	29.312977099236644	20.99236641221374
13	27.86259541984733	19.923664122137406	28.473282442748094	23.740458015267176
14	22.442748091603054	23.740458015267176	26.564885496183205	27.25190839694657
15	24.12213740458015	29.083969465648856	21.14503816793893	25.64885496183206
16	23.587786259541986	26.946564885496183	25.64885496183206	23.81679389312977
17	21.908396946564885	28.549618320610687	26.106870229007633	23.435114503816795
18	25.267175572519086	25.80152671755725	26.48854961832061	22.442748091603054
19	22.137404580152673	25.954198473282442	26.33587786259542	25.572519083969464
20	23.587786259541986	26.717557251908396	23.81679389312977	25.87786259541985
21	23.51145038167939	26.641221374045802	24.35114503816794	25.49618320610687
22	24.198473282442748	28.854961832061072	25.419847328244277	21.52671755725191
23	22.061068702290076	27.480916030534353	26.259541984732827	24.198473282442748
24	25.80152671755725	26.717557251908396	26.48854961832061	20.99236641221374
25	22.290076335877863	27.633587786259543	26.33587786259542	23.740458015267176
26	25.190839694656486	24.65648854961832	26.717557251908396	23.435114503816795
27	20.83969465648855	28.549618320610687	25.87786259541985	24.732824427480914
28	22.061068702290076	28.396946564885496	26.564885496183205	22.977099236641223
29	23.66412213740458	26.03053435114504	26.87022900763359	23.435114503816795
30	23.51145038167939	28.549618320610687	25.954198473282442	21.984732824427482
31	23.587786259541986	29.312977099236644	23.66412213740458	23.435114503816795
32	21.52671755725191	26.79389312977099	28.62595419847328	23.053435114503817
33	19.84732824427481	26.412213740458014	28.091603053435115	25.64885496183206
34	21.450381679389313	28.62595419847328	26.412213740458014	23.51145038167939
35	20.763358778625953	25.572519083969464	28.396946564885496	25.267175572519086
36	22.213740458015266	24.80916030534351	28.16793893129771	24.80916030534351
37	23.587786259541986	25.572519083969464	27.099236641221374	23.740458015267176
38	20.0	25.190839694656486	29.61832061068702	25.190839694656486
39	20.381679389312975	21.603053435114504	30.916030534351147	27.099236641221374
40	21.14503816793893	24.50381679389313	30.99236641221374	23.358778625954198
41	21.374045801526716	22.595419847328245	31.832061068702288	24.198473282442748
42	22.900763358778626	24.885496183206108	28.24427480916031	23.969465648854964
43	20.99236641221374	23.66412213740458	30.15267175572519	25.190839694656486
44	18.702290076335878	23.969465648854964	32.06106870229007	25.267175572519086
45	19.31297709923664	23.51145038167939	30.68702290076336	26.48854961832061
46	20.229007633587788	23.51145038167939	33.51145038167939	22.748091603053435
47	19.84732824427481	24.274809160305345	30.0	25.87786259541985
48	16.412213740458014	25.114503816793892	31.755725190839694	26.717557251908396
49	19.541984732824428	24.427480916030532	30.53435114503817	25.49618320610687
50	16.793893129770993	25.0381679389313	32.213740458015266	25.954198473282442
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	2.0
14	3.0
15	3.5
16	4.0
17	5.0
18	6.0
19	6.5
20	7.0
21	8.5
22	10.0
23	13.5
24	17.0
25	19.0
26	21.0
27	23.5
28	26.0
29	34.0
30	42.0
31	48.5
32	55.0
33	51.0
34	47.0
35	56.5
36	66.0
37	73.0
38	80.0
39	81.0
40	82.0
41	76.0
42	70.0
43	71.0
44	72.0
45	69.5
46	67.0
47	75.0
48	83.0
49	77.0
50	71.0
51	67.5
52	64.0
53	60.0
54	56.0
55	63.5
56	71.0
57	60.0
58	49.0
59	53.5
60	58.0
61	49.5
62	41.0
63	36.5
64	32.0
65	34.0
66	36.0
67	26.0
68	16.0
69	18.0
70	20.0
71	17.0
72	14.0
73	12.5
74	11.0
75	8.0
76	5.0
77	3.5
78	2.0
79	2.0
80	2.0
81	1.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1310.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.58015267175573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.67312348668281	89.54198473282443
2	4.923325262308313	9.31297709923664
3	0.40355125100887806	1.1450381679389312
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTCTCATGATCGGCCGGGTCAGACCATCTACGACTGGCGGCATTATCT	3	0.22900763358778628	No Hit
ATGGAGGTTCCCAGGCTAGGGGTCAAATCGGAGCTGTAGCCACGGGCCCA	3	0.22900763358778628	No Hit
CTGCATTTCTAATCATTTCCCAGGTGATGCTAATGCTGCTGGTCCACAGA	3	0.22900763358778628	No Hit
GTGTGGTCTCGGCGGCACTGGTGGGCGGTGGCGTGGCACGGCGCCTGTCT	3	0.22900763358778628	No Hit
ATCATTTAAGCACAAGAGCAAGGAGTTCATCATCAACACACCATCTATTA	3	0.22900763358778628	No Hit
CCTGCTATATTGTGAACAACTATGTTATCAGATCTTTTTATTTCATTGTG	2	0.15267175572519084	No Hit
TCCCCGTCATCGGCGCTCAGGCAGGCCGCGTTGAAGAAGATCCGGGCGGA	2	0.15267175572519084	No Hit
ACGCCACACTTCTCATCACCCACAGCCTGGGCCTCAGCTCACCACCAATT	2	0.15267175572519084	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2	0.15267175572519084	No Hit
AAACAAGAAAATAAAGAAACAAGATTTAAACTACCTAGTATCTTTGTAGC	2	0.15267175572519084	No Hit
GGCCTGTTCTATCTGCCGCGTCTGTTCGTCTATCACGCCATGAGCAACGA	2	0.15267175572519084	No Hit
TCTTACCTTCCAGAAAAGTGGGAAAAGGGGTTGGGGCGCAGAGATAAGAG	2	0.15267175572519084	No Hit
GTTAAGGATCCCGAGTTGTCTCTGCTGTGGTGCAGGTTCAGTGCCTGGCC	2	0.15267175572519084	No Hit
GCTTGGATTAAAGGCGTGCGCCACCGCGCCATTAAGACTCTCTCCCTTTC	2	0.15267175572519084	No Hit
TACTTAGATAGAAATGTAACAGAACGTGTAGAGGATCTGTGTCCTGAAAA	2	0.15267175572519084	No Hit
ACGCAGAGTACATGGGACCAACTCAGAGCCAGTTATCATGGCCACATGCT	2	0.15267175572519084	No Hit
GGACAGTTCTCAGGAAAAGGGATTTTTTTAATGATATATTAGCCATTGCT	2	0.15267175572519084	No Hit
CAGGAAGGACCCGAAGTTCATTGGAGCTTATTGGATTGGTTTCTTCGTAT	2	0.15267175572519084	No Hit
AGTAAGTTCCAAGCTTTAAGAAAATAGGCTCTTTTTACAGCTCCCATACT	2	0.15267175572519084	No Hit
TACTCATCCTTCAAAGGCTAACAGCTCTCATTCCTCAAGAACTGTCTCTT	2	0.15267175572519084	No Hit
CTACAGGTGTGTGCCACCATGCATGGCTAATTTTTGCATTCTTAGTAGAG	2	0.15267175572519084	No Hit
GTCCACGCATCGGCCGTGTACACCTCGCCAAGACTGGGAGTTGTCGAATC	2	0.15267175572519084	No Hit
ACTTCAAAATCTGATCAACAGAAAGCCTGAGTCTCAGTGCTGTCTCTTAT	2	0.15267175572519084	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	2	0.15267175572519084	No Hit
CACTTGACGACTTGAAAAATGACGAAATCACTAAAAAACGTGAAAAATGA	2	0.15267175572519084	No Hit
CGACTGGCCCCACTTGCCGGTCAGCGCGCGCCAAGCCCCTGTCTCTTATA	2	0.15267175572519084	No Hit
GTTTGGCTGTGTTCCCACCAAATCTCGTCTTGAATTTTAATTCTACCTGT	2	0.15267175572519084	No Hit
CCTATAGCATCACGGGACAAATAACAGGCCCTGAAAGAAATCAAAGCATT	2	0.15267175572519084	No Hit
ACGTATTTAAGAGGAAGTATCACCAGTTTGAAGGATGGCTGAATGTGACA	2	0.15267175572519084	No Hit
GGCCGTGGGCGCCGTGCTGCGCCGTGCCGAGATGGTGCCTGTCTCTTATA	2	0.15267175572519084	No Hit
CCTGAGTAGCTGGTATTACAGGCGTGCGCCACCATGTACAGCTAACTTTT	2	0.15267175572519084	No Hit
CCTGAGTTCAAGTGATTCTCCTGCCTCAGCCTCCCAGGTAGCCTGGCCTA	2	0.15267175572519084	No Hit
AGGTACTTGAGTGTGGATGCCAAACACTAAGATAACTTTTCCAGCTTTAG	2	0.15267175572519084	No Hit
GCACCGAGGCGCGACACCCGCCTGCCCCGTACTTTCCCGCCATCCCAAGT	2	0.15267175572519084	No Hit
GGATGGCACCGAGCCACCCCTCCCCGCGATTGTGCTACGCCTGTCTCTTA	2	0.15267175572519084	No Hit
CAGCATTATCTTCTATTACCAATTATCAAACTTCTATTTAGAGTATTAGC	2	0.15267175572519084	No Hit
ACATTTCCCTCATGGGTAAGATAAACTTGTTCCTCCCAGGAGCTGTCTCT	2	0.15267175572519084	No Hit
GAGTGGAGTGCAATGGAATAGAGTGGAATAGAGACGAGAGAATTGAAATG	2	0.15267175572519084	No Hit
CTTCTAGATTTTCTCTGGATGTCACTTCCCCCGTCAATTCTGTCCACTCA	2	0.15267175572519084	No Hit
GTTATTGAATGTAGCTGTGGTATCCTCTGATGGTTTCATGGGGGTGGGCT	2	0.15267175572519084	No Hit
GAGCAAGGGCTTGGTGGGGAGGAAAGGAGTTCTCCCCCTGTAATGAAGGT	2	0.15267175572519084	No Hit
TCACAAGGATTCCTATACACCAACAACAGACAAACAGAGAGCCAAATCAT	2	0.15267175572519084	No Hit
CTCAGGATCTGAGCCACGTCTGCAACCTACACCACAGCTCACGGAAATGC	2	0.15267175572519084	No Hit
CTATCATGTAAATAGCCAGATTTTGTCATCATCAACAGCAAGCTCTTCAC	2	0.15267175572519084	No Hit
ACTGAGCACTTACTGTGTGTTAGGCACTGCACTTTACCTGTCTCTTATAC	2	0.15267175572519084	No Hit
GGTCTAATACATTGCCTGAAAGTAGACATATAAGAGACACATATAGAAAT	2	0.15267175572519084	No Hit
AAACTGCTCTATCAAAAGGAACTTTTAACTCTGCGAATTGAACACACACA	2	0.15267175572519084	No Hit
CAATTATGCTCACAAAGTTGCATGCAAATAAAACTGTCTCTTATACACAT	2	0.15267175572519084	No Hit
GTGAAATACAATAGCTAACCCTCCAAAGACGTCATTATGCCTGTCTCTTA	2	0.15267175572519084	No Hit
CTTCCAGGGCACAAAGCTTAGGCCGTGTGTGCTTGTCATTTCTGGGTTTG	2	0.15267175572519084	No Hit
CCGGGGTCTTCTGCTGACTCTTGCTGTCTCCTGCCAGACAACACCTCCAC	2	0.15267175572519084	No Hit
AAACGCAACCGAAGAAGCACCGTCTCCTCATGACCTGTCTCTTATACACA	2	0.15267175572519084	No Hit
ACTCTACGATGCTCACCAGTGGTTGTGGGTGCTGCTCCAGAAAATCAGAT	2	0.15267175572519084	No Hit
GGGCCACACCCATGGCATATGGAGGTTCCCAGGCTAGGGGTCAAATTGGA	2	0.15267175572519084	No Hit
CTCCTTTGTATCTTGTTCCCTTGTAATAAGCACATCTGTGCAGGTCCCCA	2	0.15267175572519084	No Hit
CTGAAGAATGCATCAGAGTCTCTTAATAGCAGAATTGATCAAGCAGAGGA	2	0.15267175572519084	No Hit
GTGATTAAGTAGAAACTTGCATTAAGTTCGAGAGGTAACCTAAGGAAGCT	2	0.15267175572519084	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT	2	0.15267175572519084	No Hit
AAAGAAAGAGTATGATGTCGCCAGTTTGAATTTCAAGAGCTCATCTGTCT	2	0.15267175572519084	No Hit
GATGGAAGCCAAAGACGTCCCTGGAGCCGCCTCCCGCTGCACCCTTTCCT	2	0.15267175572519084	No Hit
TCTATCTGCTGAAATTACACATCTGATTGTGCATAGTGCCTGTCTCTTAT	2	0.15267175572519084	No Hit
GATCAGCATTGGTAATACCGTGAGAGATATCGCGGGAGATTCTTTCTGTC	2	0.15267175572519084	No Hit
CATCTGTTATTTGAGACTCTCCTAGGAGGAGCAGAAGGCAGGCGTGCTGG	2	0.15267175572519084	No Hit
GAGACAGACTGCTGCCATCTTGATCTTGAACTTCTCAGCTTCCAGAACTA	2	0.15267175572519084	No Hit
GTGTCATATCTGGGTCTGTTATTATTAGTTGACTGGCTCACATTTTCCTG	2	0.15267175572519084	No Hit
CCGTGAGACCGTTCAGCGCGTGGATGCTATCCATCTCGCGCATCTGTACT	2	0.15267175572519084	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.07633587786259542	0.0
12	0.0	0.0	0.0	0.07633587786259542	0.0
13	0.0	0.0	0.0	0.07633587786259542	0.0
14	0.0	0.0	0.0	0.07633587786259542	0.0
15	0.0	0.0	0.0	0.07633587786259542	0.0
16	0.0	0.0	0.0	0.07633587786259542	0.0
17	0.0	0.0	0.0	0.07633587786259542	0.0
18	0.0	0.0	0.0	0.07633587786259542	0.0
19	0.0	0.0	0.0	0.07633587786259542	0.0
20	0.0	0.0	0.0	0.07633587786259542	0.0
21	0.0	0.0	0.0	0.07633587786259542	0.0
22	0.0	0.0	0.0	0.07633587786259542	0.0
23	0.0	0.0	0.0	0.07633587786259542	0.0
24	0.0	0.0	0.0	0.07633587786259542	0.0
25	0.0	0.0	0.0	0.07633587786259542	0.0
26	0.0	0.0	0.0	0.07633587786259542	0.0
27	0.0	0.0	0.0	0.15267175572519084	0.0
28	0.0	0.0	0.0	0.15267175572519084	0.0
29	0.0	0.0	0.0	0.22900763358778625	0.0
30	0.0	0.0	0.0	0.4580152671755725	0.0
31	0.0	0.0	0.0	0.916030534351145	0.0
32	0.0	0.0	0.0	1.6793893129770991	0.0
33	0.0	0.0	0.0	2.4427480916030535	0.0
34	0.0	0.0	0.0	3.66412213740458	0.0
35	0.0	0.0	0.0	5.114503816793893	0.0
36	0.0	0.0	0.0	6.412213740458015	0.0
37	0.0	0.0	0.0	7.557251908396947	0.0
38	0.0	0.0	0.0	9.694656488549619	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE