FastQCFastQC Report
Thu 2 Feb 2017
SRR4062381_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062381_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954688
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG73830.7733416571696722No Hit
TCCATGTACTCTGCGTTGATACCAC65590.6870307367433129No Hit
GAGTACATGGAAGCAGTGGTATCAA31620.331207682509888No Hit
CATGTACTCTGCGTTGATACCACTG27770.2908803713883489No Hit
GCTTCCATGTACTCTGCGTTGATAC22410.23473637460615404No Hit
GTATCAACGCAGAGTACTTTTTTTT21390.2240522558155125No Hit
CCCATGTACTCTGCGTTGATACCAC19860.2080260776295502No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18930.19828467520278878No Hit
GCGTTGATACCACTGCTTCCATGTA17760.18602936247234697No Hit
GTCCTACAGTGGACATTTCTAAATT17620.1845629147952001No Hit
GTCCTAAAGTGTGTATTTCTCATTT17500.18330595964335994No Hit
CATGGAAGCAGTGGTATCAACGCAG17110.17922085539987934No Hit
GTACATGGGAAGCAGTGGTATCAAC16440.17220285580210498No Hit
TATCAACGCAGAGTACTTTTTTTTT16330.17105064691291816No Hit
CTGTAGGACGTGGAATATGGCAAGA16170.16937470671046456No Hit
GTATCAACGCAGAGTACATGGAAGC14560.15251055842327546No Hit
CTTTAGGACGTGAAATATGGCGAGG13870.1452830663001944No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13630.14276915599651405No Hit
ACTCTGCGTTGATACCACTGCTTCC12360.1294663806395388No Hit
ACGCAGAGTACATGGAAGCAGTGGT11950.1251717838707515No Hit
CAGTGGTATCAACGCAGAGTACATG11320.11857276932359054No Hit
TATCAACGCAGAGTACATGGAAGCA10320.10809814305825567No Hit
GTCCTACAGTGTGCATTTCTCATTT10240.1072601729570289No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9640.10097539719782797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA508.701547E-515.20138559
AACGGCC400.005273936314.2512977
CGAGGTT551.9600028E-413.8172674
ATCCCCG1051.366061E-913.57266412
GTCCTAG852.714769E-713.4038491
AACCGCG500.001497777513.3012127
CGCGTAA500.001498369613.30051410
GTTCAGA500.001505490713.292151
AAGACGG1301.4551915E-1113.1536655
GCCGGTT957.371273E-813.00050311
GTCGCGT604.0863076E-412.6678218
CTCGTAG604.088116E-412.66715610
CGTCGTA1051.9887011E-812.66715610
CGCAAGA1352.7284841E-1112.66118052
AAATTAG604.1098727E-412.6591891
TCCAACG1600.012.47119318
CGGTTTC1001.4394209E-712.35047813
TAATACT853.9417173E-612.29330354
TAAAGCT701.0907225E-412.2134774
CTTAACC701.09232555E-412.21155553
Produced by FastQC (version 0.11.5)