##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062381_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954688
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.142136488570088	32.0	32.0	32.0	32.0	32.0
2	30.82486529630623	32.0	32.0	32.0	32.0	32.0
3	30.80365836796943	32.0	32.0	32.0	32.0	32.0
4	30.86582317992894	32.0	32.0	32.0	32.0	32.0
5	30.670857913789636	32.0	32.0	32.0	32.0	32.0
6	34.317309948381045	36.0	36.0	36.0	32.0	36.0
7	34.1910645153181	36.0	36.0	36.0	32.0	36.0
8	34.18082556814373	36.0	36.0	36.0	32.0	36.0
9	34.34145710431052	36.0	36.0	36.0	32.0	36.0
10	34.02957405979755	36.0	36.0	36.0	32.0	36.0
11	34.34438685727693	36.0	36.0	36.0	32.0	36.0
12	34.12059960950593	36.0	36.0	36.0	32.0	36.0
13	34.231874706710464	36.0	36.0	36.0	32.0	36.0
14	34.146942247100625	36.0	36.0	36.0	32.0	36.0
15	34.09345356807669	36.0	36.0	36.0	32.0	36.0
16	34.09083595897298	36.0	36.0	36.0	32.0	36.0
17	34.016281759066835	36.0	36.0	36.0	32.0	36.0
18	34.035324629617214	36.0	36.0	36.0	32.0	36.0
19	34.02592784239458	36.0	36.0	36.0	32.0	36.0
20	34.01594971341422	36.0	36.0	36.0	32.0	36.0
21	33.96713062277938	36.0	36.0	36.0	32.0	36.0
22	33.920007374136894	36.0	36.0	36.0	32.0	36.0
23	33.860820498424616	36.0	36.0	36.0	32.0	36.0
24	33.85499660622109	36.0	36.0	36.0	32.0	36.0
25	33.35338037138835	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	41.0
5	87.0
6	161.0
7	33.0
8	123.0
9	179.0
10	134.0
11	39.0
12	90.0
13	51.0
14	182.0
15	190.0
16	327.0
17	471.0
18	629.0
19	895.0
20	1366.0
21	1943.0
22	2761.0
23	4081.0
24	5716.0
25	7995.0
26	10960.0
27	14770.0
28	19923.0
29	26705.0
30	35096.0
31	47810.0
32	69111.0
33	99068.0
34	215419.0
35	388321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57680462264982	17.245266099869685	11.588993970274585	25.58893530720592
2	15.085340892187856	20.12241238076682	39.23732666276992	25.554920064275404
3	19.72843701486033	24.407985569157216	28.230859963420073	27.632717452562378
4	12.178827719868	15.648001676182494	36.091142423131316	36.082028180818185
5	13.416655664598279	37.40713730371786	34.501124306607736	14.675082725076125
6	33.9825685275677	34.99938695374697	17.421936157258415	13.59610836142692
7	29.197486544417835	30.596867089768516	21.389842411156472	18.815803954657177
8	27.246355235816715	34.90020276064808	20.148369632451768	17.70507237108344
9	26.930315806470894	14.854301642940202	18.70848480209538	39.50689774849352
10	15.371231705846263	27.600614568202914	33.136762400171996	23.89139132577883
11	36.79360348351737	20.90351634314576	22.747422966925672	19.555457206411198
12	24.08485420599958	23.576987623243127	30.5059786028949	21.832179567862386
13	30.47867554058391	20.194314456332734	25.29328604119953	24.033723961883826
14	22.754184535964615	20.48642248002257	25.509963280755905	31.249429703256904
15	24.55894181691956	28.823282041279043	23.396454755290446	23.221321386510954
16	24.499599931626584	25.73493556434809	26.69048550368241	23.074979000342914
17	22.938829020163087	26.689740160990063	27.386245050997665	22.985185767849185
18	24.120103879975836	24.500738403705444	29.428534268644697	21.95062344767403
19	24.73161701078776	26.46479161312992	25.58960346970563	23.213987906376683
20	24.498556366721658	24.877267573993418	27.79778017130625	22.826395887978673
21	24.793978735521875	25.526918328838992	24.589661859766043	25.089441075873093
22	23.96905594185505	26.771079433153865	25.575008078018975	23.684856546972107
23	22.63852087752092	26.208982903992567	26.08898147740218	25.063514741084337
24	23.680376437417593	26.582955633711958	26.386911960761893	23.34975596810855
25	24.350005034655208	25.499165086344338	25.883791766660515	24.26703811233994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	137.0
2	371.5
3	606.0
4	606.0
5	606.0
6	1690.0
7	2774.0
8	2774.0
9	2774.0
10	2816.5
11	2859.0
12	2859.0
13	2859.0
14	2899.5
15	2940.0
16	2940.0
17	2940.0
18	4783.5
19	6627.0
20	6627.0
21	6627.0
22	11165.0
23	15703.0
24	15703.0
25	15703.0
26	24432.5
27	33162.0
28	33162.0
29	33162.0
30	43398.0
31	53634.0
32	53634.0
33	53634.0
34	60928.0
35	68222.0
36	68222.0
37	68222.0
38	75253.5
39	82285.0
40	82285.0
41	82285.0
42	93744.5
43	105204.0
44	105204.0
45	105204.0
46	128559.0
47	151914.0
48	151914.0
49	151914.0
50	138651.5
51	125389.0
52	125389.0
53	125389.0
54	109985.0
55	94581.0
56	94581.0
57	94581.0
58	85883.0
59	77185.0
60	77185.0
61	77185.0
62	67127.5
63	57070.0
64	57070.0
65	57070.0
66	46783.0
67	36496.0
68	36496.0
69	36496.0
70	27309.0
71	18122.0
72	18122.0
73	18122.0
74	13839.0
75	9556.0
76	9556.0
77	9556.0
78	7708.5
79	5861.0
80	5861.0
81	5861.0
82	4129.5
83	2398.0
84	2398.0
85	2398.0
86	1725.0
87	1052.0
88	1052.0
89	1052.0
90	720.5
91	389.0
92	389.0
93	389.0
94	230.5
95	72.0
96	72.0
97	72.0
98	261.0
99	450.0
100	450.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008798686062881277
2	0.004818328082054032
3	0.006913253335121002
4	0.014454984246162098
5	0.03372829657437822
6	0.045774116779513305
7	0.06525692163303613
8	0.08945330830595964
9	0.10170862103640141
10	0.12286736609237782
11	0.12244838104176443
12	0.1349131862975129
13	0.12381008245625796
14	0.12957112690219214
15	0.11825853053563049
16	0.11532563518133673
17	0.12705721659851177
18	0.13407521619628612
19	0.13543691761077967
20	0.1247527988201381
21	0.13271351478179258
22	0.1554434537775692
23	0.1430833947844741
24	0.1392077830663002
25	0.1357511563987397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	954688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.80056478674592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08216746953343	43.88548006677203
2	12.500108444332216	13.700260052899548
3	3.4515392532425735	5.674389013839489
4	1.3745898709688655	3.0131320511693613
5	0.6718070613711328	1.8407703195431078
6	0.4011100809885819	1.3188635387899015
7	0.258948864999304	0.9933380836994077
8	0.18176756058680812	0.7968771984052915
9	0.13888611069015147	0.684993357616933
>10	0.7563465961211965	7.859952632758521
>50	0.0897406469574495	3.4934035731931137
>100	0.07997736798972867	8.526338055587471
>500	0.008610003674074412	2.984501408469914
>1k	0.0040180017145680594	3.765856962710984
>5k	3.8266682995886274E-4	1.4618436845449532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7383	0.7733416571696722	No Hit
TCCATGTACTCTGCGTTGATACCAC	6559	0.6870307367433129	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3162	0.331207682509888	No Hit
CATGTACTCTGCGTTGATACCACTG	2777	0.2908803713883489	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2241	0.23473637460615404	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2139	0.2240522558155125	No Hit
CCCATGTACTCTGCGTTGATACCAC	1986	0.2080260776295502	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1893	0.19828467520278878	No Hit
GCGTTGATACCACTGCTTCCATGTA	1776	0.18602936247234697	No Hit
GTCCTACAGTGGACATTTCTAAATT	1762	0.1845629147952001	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1750	0.18330595964335994	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1711	0.17922085539987934	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1644	0.17220285580210498	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1633	0.17105064691291816	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1617	0.16937470671046456	No Hit
GTATCAACGCAGAGTACATGGAAGC	1456	0.15251055842327546	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1387	0.1452830663001944	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1363	0.14276915599651405	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1236	0.1294663806395388	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1195	0.1251717838707515	No Hit
CAGTGGTATCAACGCAGAGTACATG	1132	0.11857276932359054	No Hit
TATCAACGCAGAGTACATGGAAGCA	1032	0.10809814305825567	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1024	0.1072601729570289	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	964	0.10097539719782797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0474626265334852E-4
2	0.0	0.0	0.0	0.0	1.0474626265334852E-4
3	0.0	0.0	0.0	0.0	1.0474626265334852E-4
4	0.0	0.0	0.0	0.0	1.0474626265334852E-4
5	0.0	0.0	0.0	0.0	2.0949252530669705E-4
6	0.0	0.0	0.0	0.0	3.1423878796004557E-4
7	0.0	0.0	0.0	0.0	3.1423878796004557E-4
8	0.0	0.0	0.0	0.0	3.1423878796004557E-4
9	0.0	0.0	0.0	0.0	3.1423878796004557E-4
10	0.0	0.0	0.0	1.0474626265334852E-4	3.1423878796004557E-4
11	0.0	0.0	0.0	2.0949252530669705E-4	3.1423878796004557E-4
12	0.0	0.0	0.0	2.0949252530669705E-4	3.1423878796004557E-4
13	0.0	0.0	0.0	2.0949252530669705E-4	3.1423878796004557E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	50	8.701547E-5	15.2013855	9
AACGGCC	40	0.0052739363	14.251297	7
CGAGGTT	55	1.9600028E-4	13.817267	4
ATCCCCG	105	1.366061E-9	13.572664	12
GTCCTAG	85	2.714769E-7	13.403849	1
AACCGCG	50	0.0014977775	13.301212	7
CGCGTAA	50	0.0014983696	13.300514	10
GTTCAGA	50	0.0015054907	13.29215	1
AAGACGG	130	1.4551915E-11	13.153665	5
GCCGGTT	95	7.371273E-8	13.000503	11
GTCGCGT	60	4.0863076E-4	12.667821	8
CTCGTAG	60	4.088116E-4	12.667156	10
CGTCGTA	105	1.9887011E-8	12.667156	10
CGCAAGA	135	2.7284841E-11	12.6611805	2
AAATTAG	60	4.1098727E-4	12.659189	1
TCCAACG	160	0.0	12.471193	18
CGGTTTC	100	1.4394209E-7	12.350478	13
TAATACT	85	3.9417173E-6	12.2933035	4
TAAAGCT	70	1.0907225E-4	12.213477	4
CTTAACC	70	1.09232555E-4	12.2115555	3
>>END_MODULE