FastQCFastQC Report
Thu 2 Feb 2017
SRR4062381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954688
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52120.5459375209492525No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39260.4112338271770463No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18240.19105718307970773No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA18190.19053345176644096No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG15150.15869058791982302No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA11150.11679208285848362No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11040.11563987396929679No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10690.11197375477642957No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10030.10506050144130857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1700.024.5881186
CGTCGTA1650.022.6665610
CCGTCGT1800.021.9998959
ACGTGTA500.002580115321.9998958
TCGTATC500.002580115321.99989544
TACCGTC1800.021.9998957
CGTAAAT602.872258E-421.9998933
GTCGTAG1750.021.37132611
ACCGTCG1750.021.3713268
GTCCTAC9700.020.4154821
TCCTACA10200.020.2754752
TATAGGA1452.9467628E-1019.7250772
CGACGGT1352.5956979E-919.5554627
GACGGTA1352.5956979E-919.5554628
GTATAGT808.975537E-519.2529341
GTAGGAC12200.018.9343383
GATATAC4300.018.9331171
CATAAGG708.121874E-418.8570543
CTAGGAC1759.094947E-1218.8570543
ACGACGG1404.0781742E-918.8570546
Produced by FastQC (version 0.11.5)