##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954688
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246725631829456	32.0	32.0	32.0	32.0	32.0
2	31.378712207548435	32.0	32.0	32.0	32.0	32.0
3	31.49708386404773	32.0	32.0	32.0	32.0	32.0
4	31.60776400248039	32.0	32.0	32.0	32.0	32.0
5	31.536621388348863	32.0	32.0	32.0	32.0	32.0
6	35.14883082221626	36.0	36.0	36.0	36.0	36.0
7	35.153380999865924	36.0	36.0	36.0	36.0	36.0
8	35.10012485754508	36.0	36.0	36.0	36.0	36.0
9	35.233552741838174	36.0	36.0	36.0	36.0	36.0
10	35.101765184018234	36.0	36.0	36.0	36.0	36.0
11	35.23039673694443	36.0	36.0	36.0	36.0	36.0
12	35.14895337534357	36.0	36.0	36.0	36.0	36.0
13	35.18878523664276	36.0	36.0	36.0	36.0	36.0
14	35.15122741670577	36.0	36.0	36.0	36.0	36.0
15	35.11037113695783	36.0	36.0	36.0	36.0	36.0
16	35.13033053730643	36.0	36.0	36.0	36.0	36.0
17	35.10828040155527	36.0	36.0	36.0	36.0	36.0
18	35.11511719011866	36.0	36.0	36.0	36.0	36.0
19	35.09942934236106	36.0	36.0	36.0	36.0	36.0
20	35.074766834819336	36.0	36.0	36.0	36.0	36.0
21	35.05444710732721	36.0	36.0	36.0	36.0	36.0
22	35.036955528926725	36.0	36.0	36.0	36.0	36.0
23	34.984077520614065	36.0	36.0	36.0	36.0	36.0
24	34.976768326406116	36.0	36.0	36.0	36.0	36.0
25	34.94641495441443	36.0	36.0	36.0	32.0	36.0
26	34.90255140946571	36.0	36.0	36.0	32.0	36.0
27	34.87182933062948	36.0	36.0	36.0	32.0	36.0
28	34.82807681671918	36.0	36.0	36.0	32.0	36.0
29	34.8096655661326	36.0	36.0	36.0	32.0	36.0
30	34.78892475866461	36.0	36.0	36.0	32.0	36.0
31	34.76769478615003	36.0	36.0	36.0	32.0	36.0
32	34.73819404873634	36.0	36.0	36.0	32.0	36.0
33	34.703851939062815	36.0	36.0	36.0	32.0	36.0
34	34.68997096433599	36.0	36.0	36.0	32.0	36.0
35	34.644802280954615	36.0	36.0	36.0	32.0	36.0
36	34.591968266072264	36.0	36.0	36.0	32.0	36.0
37	34.55276383488637	36.0	36.0	36.0	32.0	36.0
38	34.47846940571161	36.0	36.0	36.0	32.0	36.0
39	34.38837400281558	36.0	36.0	36.0	32.0	36.0
40	34.339245910705905	36.0	36.0	36.0	32.0	36.0
41	34.34403071998391	36.0	36.0	36.0	32.0	36.0
42	34.24691103271435	36.0	36.0	36.0	32.0	36.0
43	34.236385080780316	36.0	36.0	36.0	32.0	36.0
44	34.22049821512368	36.0	36.0	36.0	32.0	36.0
45	34.095963288529866	36.0	36.0	36.0	32.0	36.0
46	34.128101536837164	36.0	36.0	36.0	32.0	36.0
47	34.09060970704565	36.0	36.0	36.0	32.0	36.0
48	34.04674930448481	36.0	36.0	36.0	32.0	36.0
49	34.056406909901455	36.0	36.0	36.0	32.0	36.0
50	33.494726025675405	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	19.0
22	80.0
23	228.0
24	580.0
25	1438.0
26	3079.0
27	5617.0
28	9900.0
29	15712.0
30	23642.0
31	35609.0
32	53716.0
33	84065.0
34	172375.0
35	548622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42479563433238	17.71273881947202	11.994376342925747	25.86808920326985
2	14.678289798962549	20.61063344814005	38.867100753366685	25.843975999530716
3	19.250199804961028	24.695788436137306	28.533195905229075	27.520815853672588
4	11.979411074612859	16.098138868405176	36.273526010591944	35.64892404639002
5	13.420824394985587	37.64496882751223	34.14131108802038	14.7928956894818
6	34.11977651103304	35.129331565197894	17.267288028005254	13.483603895763807
7	29.409189180130053	30.642262184085272	21.184722129114434	18.76382650667024
8	27.32358634443923	35.09209291412482	19.76844791177851	17.815872829657437
9	27.08078450760877	14.672018502379835	18.45000670376081	39.79719028625059
10	15.392987028222832	27.80039133203727	32.937776530133405	23.86884510960649
11	37.072111517061074	20.909134712073474	22.54380488704163	19.474948883823824
12	24.245617416370585	23.657886136622643	30.34593500703895	21.75056143996782
13	30.79435375745793	20.062575417309112	25.1892764966146	23.953794328618354
14	22.933984715425353	20.438090768921366	25.298841087350006	31.329083428303278
15	24.70042568881142	28.929451297177717	23.107235033853993	23.26288798015687
16	24.657374974860897	25.776274552523965	26.456287289669504	23.110063182945634
17	23.114881511027686	26.774401689347727	27.027573406180867	23.083143393443724
18	24.221525775960316	24.59714587383522	29.194354595428035	21.98697375477643
19	25.04388868405175	26.400143292887314	25.339273144734197	23.21669487832674
20	24.732373298920695	24.990468090098545	27.47085958972984	22.806299021250922
21	24.999895253737346	25.60658560702554	24.240380103237914	25.153139035999196
22	24.26614768385064	26.78602852450225	25.252229000469264	23.69559479117785
23	22.731824428504392	26.135868472212913	25.91799624589395	25.21431085338875
24	23.849257558490315	26.6610662331568	26.019600120667697	23.47007608768519
25	24.636425722330227	25.376772306764096	25.654873634108732	24.331928336796942
26	22.35044328618355	26.543540926459748	26.94985167929208	24.156164108064623
27	24.334861232151237	26.618015519206274	25.98251994368841	23.06460330495408
28	23.3077193805725	25.78643494000134	25.89599953073674	25.009846148689412
29	23.25387980156868	26.07197325199437	26.3637963397466	24.310350606690353
30	23.923941643762152	25.755220553730645	26.72548518468861	23.595352617818595
31	23.819614366159417	25.391751022323522	25.815973386069587	24.972661225447474
32	23.181709626600522	26.691023664275658	25.940830931152377	24.18643577797144
33	22.74586042769994	26.04222531340082	26.887632399275997	24.324281859623248
34	23.133526345779984	26.85914141583428	27.23738017027553	22.76995206811021
35	24.306370248709527	26.370814339344374	26.512326540189047	22.810488871757055
36	22.951896326339078	26.9174850841322	26.54553110545016	23.585087484078567
37	24.186016792920828	26.539770061004226	25.71635969028625	23.557853455788695
38	23.667837031574713	26.89685007038949	25.175240497419054	24.260072400616746
39	24.338841590132066	25.707142019172757	25.990061674599453	23.963954716095728
40	25.46015033183616	25.858081383656227	25.453132332238386	23.228635952269222
41	23.37748039149963	25.698971810685794	27.253511094724143	23.67003670309043
42	25.305230609371858	26.934139739894082	25.947639438224847	21.812990212509217
43	23.757604578668634	25.619259904806597	26.157655694844806	24.46547982167996
44	23.100426526781526	26.32493547630221	26.993426124555874	23.581211872360395
45	23.872511228799357	26.467704632298723	25.972988033786955	23.68679610511497
46	23.10107920187454	25.5226058383533	27.000053420649913	24.376261539122247
47	24.94458922705638	25.042736475162563	26.83599249178789	23.176681805993162
48	23.60917912448884	27.4601754709392	25.11134527720051	23.819300127371456
49	23.466409968492325	25.719292585640545	27.170656800965343	23.64364064490179
50	22.907379164711404	28.118610477978145	24.599136052825635	24.374874304484816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	16.0
3	79.0
4	142.0
5	163.0
6	184.0
7	164.5
8	145.0
9	198.0
10	251.0
11	373.0
12	495.0
13	832.5
14	1170.0
15	1586.0
16	2002.0
17	2372.5
18	2743.0
19	3007.0
20	3271.0
21	3862.5
22	4454.0
23	5279.5
24	6105.0
25	6797.5
26	7490.0
27	9754.5
28	12019.0
29	14471.0
30	16923.0
31	19828.0
32	22733.0
33	27396.5
34	32060.0
35	37489.0
36	42918.0
37	45511.5
38	48105.0
39	47650.0
40	47195.0
41	47844.0
42	48493.0
43	49524.5
44	50556.0
45	59072.0
46	67588.0
47	74595.0
48	81602.0
49	79076.0
50	76550.0
51	69319.5
52	62089.0
53	59602.5
54	57116.0
55	56950.0
56	56784.0
57	54267.0
58	51750.0
59	47283.5
60	42817.0
61	37766.5
62	32716.0
63	28068.5
64	23421.0
65	20063.0
66	16705.0
67	14338.5
68	11972.0
69	10601.5
70	9231.0
71	7251.0
72	5271.0
73	4656.0
74	4041.0
75	2953.5
76	1866.0
77	1610.0
78	1354.0
79	1155.0
80	956.0
81	767.0
82	578.0
83	477.0
84	376.0
85	309.5
86	243.0
87	179.0
88	115.0
89	73.5
90	32.0
91	21.5
92	11.0
93	11.0
94	11.0
95	7.5
96	4.0
97	5.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015607193135348932
2	0.004189850506133941
3	7.332238385734397E-4
4	0.0
5	0.0
6	0.0010474626265334853
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0474626265334854E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	954688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.70254558271859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3455924897053	47.968363979871626
2	12.604514567519004	15.050432110306877
3	3.3480967977300664	5.996697050455004
4	1.3257219103388425	3.1659589112805406
5	0.6401878590940613	1.9110422419533108
6	0.37562816734394155	1.345557466980283
7	0.24537608279226417	1.0254703737469857
8	0.1698766575832552	0.8113655114243338
9	0.12452704761892451	0.6691123563075189
>10	0.6774786828447643	7.55484669525903
>50	0.07194517930019399	2.9971699822287525
>100	0.06435462478327429	7.535005430569044
>500	0.00511310702731653	2.012436528144614
>1k	0.0014105122833976637	1.4079055635492317
>5k	1.7631403542470796E-4	0.5486357979228856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5212	0.5459375209492525	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3926	0.4112338271770463	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1824	0.19105718307970773	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1819	0.19053345176644096	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1515	0.15869058791982302	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1115	0.11679208285848362	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1104	0.11563987396929679	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1069	0.11197375477642957	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1003	0.10506050144130857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0474626265334852E-4
2	0.0	0.0	0.0	0.0	1.0474626265334852E-4
3	0.0	0.0	0.0	0.0	1.0474626265334852E-4
4	0.0	0.0	0.0	0.0	1.0474626265334852E-4
5	0.0	0.0	0.0	0.0	1.0474626265334852E-4
6	0.0	0.0	0.0	0.0	1.0474626265334852E-4
7	0.0	0.0	0.0	0.0	1.0474626265334852E-4
8	0.0	0.0	0.0	0.0	1.0474626265334852E-4
9	0.0	0.0	0.0	1.0474626265334852E-4	1.0474626265334852E-4
10	0.0	0.0	0.0	3.1423878796004557E-4	1.0474626265334852E-4
11	0.0	0.0	0.0	4.189850506133941E-4	1.0474626265334852E-4
12	0.0	0.0	0.0	4.189850506133941E-4	2.0949252530669705E-4
13	0.0	0.0	0.0	4.189850506133941E-4	5.237313132667427E-4
14	0.0	0.0	0.0	4.189850506133941E-4	5.237313132667427E-4
15	0.0	0.0	0.0	5.237313132667427E-4	6.284775759200911E-4
16	0.0	0.0	0.0	5.237313132667427E-4	6.284775759200911E-4
17	0.0	0.0	0.0	6.284775759200911E-4	6.284775759200911E-4
18	0.0	0.0	0.0	6.284775759200911E-4	7.332238385734397E-4
19	0.0	0.0	0.0	6.284775759200911E-4	7.332238385734397E-4
20	0.0	0.0	0.0	6.284775759200911E-4	7.332238385734397E-4
21	0.0	0.0	0.0	0.0010474626265334853	7.332238385734397E-4
22	0.0	0.0	0.0	0.001780686465106925	7.332238385734397E-4
23	0.0	0.0	0.0	0.0028281490916404104	7.332238385734397E-4
24	0.0	0.0	0.0	0.004294596768787289	7.332238385734397E-4
25	0.0	0.0	0.0	0.005342059395320775	8.379701012267882E-4
26	0.0	0.0	0.0	0.0071227458604277	8.379701012267882E-4
27	0.0	0.0	0.0	0.00848444727492123	8.379701012267882E-4
28	0.0	0.0	0.0	0.012255312730441777	8.379701012267882E-4
29	0.0	0.0	0.0	0.01696889454984246	8.379701012267882E-4
30	0.0	0.0	0.0	0.02566283435007039	8.379701012267882E-4
31	0.0	0.0	0.0	0.045250385466246566	8.379701012267882E-4
32	0.0	0.0	0.0	0.07248441375611718	8.379701012267882E-4
33	0.0	0.0	0.0	0.09846148689414762	8.379701012267882E-4
34	0.0	0.0	0.0	0.1314565596299524	8.379701012267882E-4
35	0.0	0.0	0.0	0.16968894549842461	8.379701012267882E-4
36	0.0	0.0	0.0	0.2238427632902058	8.379701012267882E-4
37	0.0	0.0	0.0	0.30784926593819134	8.379701012267882E-4
38	0.0	0.0	0.0	0.41133857343969965	9.427163638801368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	170	0.0	24.588118	6
CGTCGTA	165	0.0	22.66656	10
CCGTCGT	180	0.0	21.999895	9
ACGTGTA	50	0.0025801153	21.999895	8
TCGTATC	50	0.0025801153	21.999895	44
TACCGTC	180	0.0	21.999895	7
CGTAAAT	60	2.872258E-4	21.999893	3
GTCGTAG	175	0.0	21.371326	11
ACCGTCG	175	0.0	21.371326	8
GTCCTAC	970	0.0	20.415482	1
TCCTACA	1020	0.0	20.275475	2
TATAGGA	145	2.9467628E-10	19.725077	2
CGACGGT	135	2.5956979E-9	19.555462	7
GACGGTA	135	2.5956979E-9	19.555462	8
GTATAGT	80	8.975537E-5	19.252934	1
GTAGGAC	1220	0.0	18.934338	3
GATATAC	430	0.0	18.933117	1
CATAAGG	70	8.121874E-4	18.857054	3
CTAGGAC	175	9.094947E-12	18.857054	3
ACGACGG	140	4.0781742E-9	18.857054	6
>>END_MODULE