FastQCFastQC Report
Thu 2 Feb 2017
SRR4062380_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062380_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6715037
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCA100850.15018532288057385No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG95480.14218834535088934No Hit
GAATAGGACCGCGGTTCTATTTTGT94600.14087785368866917No Hit
GCGCAAGACGGACCAGAGCGAAAGC90590.13490618145514313No Hit
CTATTGGAGCTGGAATTACCGCGGC88130.13124276158120946No Hit
GGGTAGGCACACGCTGAGCCAGTCA86490.12880048166525368No Hit
GTATCTGATCGTCTTCGAACCTCCG86420.12869623801030433No Hit
GATTAAGAGGGACGGCCGGGGGCAT85330.12707301538323618No Hit
GAACTACGACGGTATCTGATCGTCT83970.1250477100870777No Hit
ATCAGATACCGTCGTAGTTCCGACC83390.12418397694606895No Hit
TCGTAGTTCCGACCATAAACGATGC81860.12190550848789068No Hit
GTCCTATTCCATTATTCCTAGCTGC76710.11423615387376124No Hit
GAATAACGCCGCCGCATCGCCAGTC73630.1096494330559906No Hit
GTATCAACGCAGAGTACTTTTTTTT72880.10853253675296205No Hit
GTACATGGGGAATAATTGCAATCCC70640.10519673979458341No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC67570.10062491092751984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA1600.014.251492513
TCGCGTA9900.011.8997949
CGGTCCA20200.011.85228510
ACGGTAT15250.011.8368649
AGAATTT19800.011.75821117
TTAGACG907.4887466E-611.6086054
GCGTAAC10200.011.36317311
AAGAATT21500.011.35852916
GAATTTC20450.011.24532918
CGCGTAA10400.011.23633710
GGTTCTA16700.011.20776213
GGTCCAA22000.010.92541111
GCGTTAT7300.010.9243421
AGGCCCG19850.010.91257610
AAGACGG17350.010.8950395
CGTCTTA2800.010.858767515
GAGGTTA8550.010.7765765
CCGACCA16700.010.7522239
CGACCAT16800.010.68822110
CGTTATT7200.010.6823262
Produced by FastQC (version 0.11.5)