##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062380_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6715037 Sequences flagged as poor quality 0 Sequence length 25 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.256258156135253 32.0 32.0 32.0 32.0 32.0 2 30.895061039872157 32.0 32.0 32.0 32.0 32.0 3 30.869627672937618 32.0 32.0 32.0 32.0 32.0 4 30.955887510374104 32.0 32.0 32.0 32.0 32.0 5 30.719138256423605 32.0 32.0 32.0 32.0 32.0 6 34.3982728613409 36.0 36.0 36.0 32.0 36.0 7 34.29802099377859 36.0 36.0 36.0 32.0 36.0 8 34.299111829167884 36.0 36.0 36.0 32.0 36.0 9 34.47254274250462 36.0 36.0 36.0 32.0 36.0 10 34.120936489255385 36.0 36.0 36.0 32.0 36.0 11 34.42512587793634 36.0 36.0 36.0 32.0 36.0 12 34.19866174974166 36.0 36.0 36.0 32.0 36.0 13 34.335236872112546 36.0 36.0 36.0 32.0 36.0 14 34.23301122540352 36.0 36.0 36.0 32.0 36.0 15 34.15431024430692 36.0 36.0 36.0 32.0 36.0 16 34.16242531500571 36.0 36.0 36.0 32.0 36.0 17 34.07405781978565 36.0 36.0 36.0 32.0 36.0 18 34.091832256471555 36.0 36.0 36.0 32.0 36.0 19 34.07745065291524 36.0 36.0 36.0 32.0 36.0 20 34.078138661037904 36.0 36.0 36.0 32.0 36.0 21 34.05434519571523 36.0 36.0 36.0 32.0 36.0 22 34.00756436636164 36.0 36.0 36.0 32.0 36.0 23 33.97464973015041 36.0 36.0 36.0 32.0 36.0 24 33.93507824305361 36.0 36.0 36.0 32.0 36.0 25 33.48648816082473 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 30.0 4 259.0 5 610.0 6 1116.0 7 284.0 8 998.0 9 1159.0 10 800.0 11 256.0 12 524.0 13 426.0 14 804.0 15 963.0 16 1639.0 17 2328.0 18 3259.0 19 4631.0 20 7442.0 21 10783.0 22 16861.0 23 25302.0 24 36614.0 25 52672.0 26 74862.0 27 97603.0 28 134526.0 29 180155.0 30 235345.0 31 327436.0 32 474349.0 33 691292.0 34 1552391.0 35 2777316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.533731619098056 16.776941404810707 11.66172855465875 26.02759842143249 2 17.655206758919586 19.03515830178735 35.88209092123505 27.42754401805802 3 19.78192687111302 22.49246431720161 26.8029791264565 30.92262968522887 4 13.518170573047572 14.698075592622297 33.747675571174625 38.0360782631555 5 16.431902012311028 34.85315850003277 31.779631980073532 16.935307507582664 6 36.286826667048075 33.02500423123774 16.240548249221096 14.447620852493092 7 32.460606201496326 28.716395944668815 19.580888311491684 19.242109542343176 8 29.37566560269602 30.994918130402603 18.84196868602482 20.78744758087655 9 27.44773929735929 14.922134020056113 18.16721814965854 39.46290853292606 10 17.402269353646908 25.047039648086777 28.992600089338055 28.558090908928257 11 38.49663242358897 20.596390454281757 21.190690570472945 19.716286551656324 12 26.669546592164377 22.364286617502152 26.581433994135566 24.384732796197902 13 29.73835160077149 18.404786867960357 24.734188795732706 27.122672735535446 14 24.780281518777166 19.15551225598515 23.22831100065546 32.83589522458223 15 26.275306118493535 26.223542163321838 21.01061966165576 26.490532056528863 16 27.54778461225608 24.577733502258816 22.272312133264673 25.602169752220426 17 25.2863749958438 24.92052531940092 23.852617613655763 25.940482071099513 18 26.51581607996337 23.374862500150975 24.88751367915673 25.22180774072893 19 26.6655602298287 23.967792846393763 23.929231584089496 25.43741533968804 20 26.944781159841657 23.06859302663655 23.47215947636407 26.514466337157728 21 28.003704506140153 23.112590355490337 23.073836485508604 25.809868652860906 22 27.04984657750155 23.138087407423757 23.52594640369133 26.286119611383363 23 25.195324849472513 22.948686441132928 24.395131224656087 27.460857484738476 24 25.787279285781388 23.983465725827497 23.82258163126839 26.406673357122727 25 25.96609801511497 23.357056680603243 23.84155394609841 26.835291358183376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 405.0 1 405.0 2 1162.5 3 1920.0 4 1920.0 5 1920.0 6 4038.0 7 6156.0 8 6156.0 9 6156.0 10 6375.0 11 6594.0 12 6594.0 13 6594.0 14 7628.0 15 8662.0 16 8662.0 17 8662.0 18 14230.5 19 19799.0 20 19799.0 21 19799.0 22 36861.0 23 53923.0 24 53923.0 25 53923.0 26 88468.5 27 123014.0 28 123014.0 29 123014.0 30 158759.0 31 194504.0 32 194504.0 33 194504.0 34 265014.0 35 335524.0 36 335524.0 37 335524.0 38 398647.0 39 461770.0 40 461770.0 41 461770.0 42 552092.5 43 642415.0 44 642415.0 45 642415.0 46 751865.0 47 861315.0 48 861315.0 49 861315.0 50 896499.5 51 931684.0 52 931684.0 53 931684.0 54 883097.5 55 834511.0 56 834511.0 57 834511.0 58 794221.0 59 753931.0 60 753931.0 61 753931.0 62 676059.0 63 598187.0 64 598187.0 65 598187.0 66 497038.5 67 395890.0 68 395890.0 69 395890.0 70 304498.0 71 213106.0 72 213106.0 73 213106.0 74 168666.0 75 124226.0 76 124226.0 77 124226.0 78 105108.0 79 85990.0 80 85990.0 81 85990.0 82 60667.0 83 35344.0 84 35344.0 85 35344.0 86 26154.0 87 16964.0 88 16964.0 89 16964.0 90 11318.0 91 5672.0 92 5672.0 93 5672.0 94 3333.0 95 994.0 96 994.0 97 994.0 98 1765.5 99 2537.0 100 2537.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008860710670693252 2 0.003514500366863206 3 0.004720748374134052 4 0.015219573622602525 5 0.035100327816510915 6 0.045465125508615964 7 0.06646277600555292 8 0.08774337356592375 9 0.09618710961681969 10 0.11807827715617947 11 0.11895690224789529 12 0.13171930400383497 13 0.11944833662122785 14 0.12320110819940382 15 0.11417658607093305 16 0.11232998418325917 17 0.12187572458647658 18 0.12905364482727347 19 0.13142146498969404 20 0.120431205367893 21 0.12821969558767882 22 0.1492620219367369 23 0.13827176231493585 24 0.13353612199009476 25 0.12961953895414127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6715037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.517279450386695 #Duplication Level Percentage of deduplicated Percentage of total 1 69.49600640194576 23.29317067259879 2 14.774628382929253 9.904106965725093 3 5.707492712194348 5.738988845869902 4 3.0121578535350753 4.0383734610244835 5 1.610921869398424 2.699685923468316 6 1.0307637542084531 2.072903807828066 7 0.7340990026806401 1.722350099196803 8 0.5366023782788866 1.4388361493212443 9 0.3867147905005424 1.1665464930723983 >10 2.3664950415186796 14.85445829866169 >50 0.19211843025080422 4.461048256291102 >100 0.11416505488947876 7.676133214140046 >500 0.016513587993807463 3.9303928481232075 >1k 0.01967383494111605 13.211124158570776 >5k 0.0016023937960933752 3.641424146000306 >10k+ 4.451093878037153E-5 0.15045666010772232 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTTGCGCCGGTCCAAGAATTTCA 10085 0.15018532288057385 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 9548 0.14218834535088934 No Hit GAATAGGACCGCGGTTCTATTTTGT 9460 0.14087785368866917 No Hit GCGCAAGACGGACCAGAGCGAAAGC 9059 0.13490618145514313 No Hit CTATTGGAGCTGGAATTACCGCGGC 8813 0.13124276158120946 No Hit GGGTAGGCACACGCTGAGCCAGTCA 8649 0.12880048166525368 No Hit GTATCTGATCGTCTTCGAACCTCCG 8642 0.12869623801030433 No Hit GATTAAGAGGGACGGCCGGGGGCAT 8533 0.12707301538323618 No Hit GAACTACGACGGTATCTGATCGTCT 8397 0.1250477100870777 No Hit ATCAGATACCGTCGTAGTTCCGACC 8339 0.12418397694606895 No Hit TCGTAGTTCCGACCATAAACGATGC 8186 0.12190550848789068 No Hit GTCCTATTCCATTATTCCTAGCTGC 7671 0.11423615387376124 No Hit GAATAACGCCGCCGCATCGCCAGTC 7363 0.1096494330559906 No Hit GTATCAACGCAGAGTACTTTTTTTT 7288 0.10853253675296205 No Hit GTACATGGGGAATAATTGCAATCCC 7064 0.10519673979458341 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 6757 0.10062491092751984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4891950707047482E-4 2 0.0 0.0 0.0 0.0 1.4891950707047482E-4 3 0.0 0.0 0.0 0.0 1.638114577775223E-4 4 0.0 1.4891950707047482E-5 0.0 0.0 1.638114577775223E-4 5 1.4891950707047482E-5 1.4891950707047482E-5 0.0 0.0 2.2337926060571223E-4 6 1.4891950707047482E-5 1.4891950707047482E-5 0.0 0.0 2.6805511272685466E-4 7 1.4891950707047482E-5 1.4891950707047482E-5 0.0 1.4891950707047482E-5 2.6805511272685466E-4 8 1.4891950707047482E-5 1.4891950707047482E-5 0.0 2.9783901414094964E-5 2.9783901414094963E-4 9 1.4891950707047482E-5 1.4891950707047482E-5 0.0 5.956780282818993E-5 2.9783901414094963E-4 10 1.4891950707047482E-5 1.4891950707047482E-5 0.0 7.445975353523741E-5 3.276229155550446E-4 11 1.4891950707047482E-5 1.4891950707047482E-5 0.0 8.93517042422849E-5 3.425148662620921E-4 12 1.4891950707047482E-5 1.4891950707047482E-5 0.0 8.93517042422849E-5 6.999216832312316E-4 13 1.4891950707047482E-5 1.4891950707047482E-5 0.0 1.0424365494933237E-4 8.488411903017065E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGTA 160 0.0 14.2514925 13 TCGCGTA 990 0.0 11.899794 9 CGGTCCA 2020 0.0 11.852285 10 ACGGTAT 1525 0.0 11.836864 9 AGAATTT 1980 0.0 11.758211 17 TTAGACG 90 7.4887466E-6 11.608605 4 GCGTAAC 1020 0.0 11.363173 11 AAGAATT 2150 0.0 11.358529 16 GAATTTC 2045 0.0 11.245329 18 CGCGTAA 1040 0.0 11.236337 10 GGTTCTA 1670 0.0 11.207762 13 GGTCCAA 2200 0.0 10.925411 11 GCGTTAT 730 0.0 10.924342 1 AGGCCCG 1985 0.0 10.912576 10 AAGACGG 1735 0.0 10.895039 5 CGTCTTA 280 0.0 10.8587675 15 GAGGTTA 855 0.0 10.776576 5 CCGACCA 1670 0.0 10.752223 9 CGACCAT 1680 0.0 10.688221 10 CGTTATT 720 0.0 10.682326 2 >>END_MODULE