FastQCFastQC Report
Thu 2 Feb 2017
SRR4062380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6715037
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC105040.15642505022682673No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT91760.1366485396878677No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA89180.13280641640544946No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT88410.1316597362010068No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA87650.13052794794727118No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT87220.12988759406686812No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG86640.12902386092585938No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA85420.12720704293959958No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC83580.12446692400950286No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA81280.12104177534688193No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC78580.11702094865597912No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG70500.10498825248468474No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT67320.10025261215984366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121700.019.6895281
ACCGTCG26500.017.9319828
CGCAATA32000.017.80615836
TACCGTC26900.017.7471187
ATACCGT27000.017.4369456
ATACGAA32450.017.42363740
CGTCGTA27450.017.39153110
TACGAAT32750.017.26403241
CGAATGC33700.016.84264243
CTAGCGG34250.016.76487529
TAGGACG10150.016.6896924
TCTAGCG34650.016.63483228
CCGTCGT28400.016.4999149
TAGCGGC35150.016.46079630
GCAATAC35300.016.26620537
TGTAGGA18150.016.1217292
CAATACG35250.016.10204338
TCTATAC5500.016.0000343
GTAGGAC19000.015.9789823
AATACGA35600.015.88193839
Produced by FastQC (version 0.11.5)