FastQCFastQC Report
Thu 2 Feb 2017
SRR4062379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617114
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35060.13396435921400443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG508.733976E-515.1966495
GTATCAA35450.012.694741
ACCGTTA1001.4433499E-712.35034758
CCTAACG550.003081975312.0843142
AACCGCG2150.011.930577
CGACCAT2450.011.633203510
GCGAAAT1157.066592E-811.56687112
ACCGTCG3450.011.5655438
GACGGTT752.0743033E-411.4003227
AGAACCG3750.011.1442095
GTATTAT1451.2314558E-911.1312171
GCGTTAT1451.2314558E-911.1312171
TACCGTC3800.011.0003117
ACGGTAT3300.010.9399119
CCGTCGT3100.010.7263159
TTCGGAA2400.010.69066418
CGAAATT1252.2363201E-710.6419313
ATTCGGC3750.010.64091111
CGTCGTA3350.010.49303310
GTTTTCG2400.010.29353215
Produced by FastQC (version 0.11.5)