##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062379_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2617114
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234338282550933	32.0	32.0	32.0	32.0	32.0
2	30.99158080236474	32.0	32.0	32.0	32.0	32.0
3	30.98752213315889	32.0	32.0	32.0	32.0	32.0
4	30.97879725529725	32.0	32.0	32.0	32.0	32.0
5	30.89689826274285	32.0	32.0	32.0	32.0	32.0
6	34.442540905745794	36.0	36.0	36.0	32.0	36.0
7	34.38086074966547	36.0	36.0	36.0	32.0	36.0
8	34.36760989395189	36.0	36.0	36.0	32.0	36.0
9	34.48490703882215	36.0	36.0	36.0	32.0	36.0
10	34.24826354526398	36.0	36.0	36.0	32.0	36.0
11	34.46869987321912	36.0	36.0	36.0	32.0	36.0
12	34.3231674279378	36.0	36.0	36.0	32.0	36.0
13	34.39898911549134	36.0	36.0	36.0	32.0	36.0
14	34.32568928980549	36.0	36.0	36.0	32.0	36.0
15	34.260989777289026	36.0	36.0	36.0	32.0	36.0
16	34.281428321425814	36.0	36.0	36.0	32.0	36.0
17	34.21862211581154	36.0	36.0	36.0	32.0	36.0
18	34.22033086827704	36.0	36.0	36.0	32.0	36.0
19	34.20272559773858	36.0	36.0	36.0	32.0	36.0
20	34.171896982706905	36.0	36.0	36.0	32.0	36.0
21	34.15139309942173	36.0	36.0	36.0	32.0	36.0
22	34.108819867992	36.0	36.0	36.0	32.0	36.0
23	34.077581641456966	36.0	36.0	36.0	32.0	36.0
24	34.04513101072403	36.0	36.0	36.0	32.0	36.0
25	33.50552020278826	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	73.0
5	231.0
6	434.0
7	90.0
8	390.0
9	516.0
10	313.0
11	85.0
12	183.0
13	156.0
14	437.0
15	665.0
16	1157.0
17	1604.0
18	2087.0
19	2705.0
20	3578.0
21	4674.0
22	6327.0
23	8932.0
24	12131.0
25	17093.0
26	23360.0
27	30981.0
28	43512.0
29	60059.0
30	82391.0
31	119248.0
32	183717.0
33	284294.0
34	675252.0
35	1050427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38587289225641	17.608787197666857	11.102266882392819	25.90307302768392
2	17.44285129108089	19.573088903765793	37.354950187846384	25.629109617306934
3	19.442278056234557	23.975746302163202	27.79175262695534	28.79022301464691
4	12.948212576263348	15.353328836669844	35.992211727884346	35.706246859182464
5	15.060395235041085	36.15970241558007	33.24700164279932	15.53290070657953
6	33.39517128782096	35.68685810311024	17.06792529139209	13.850045317676724
7	30.305199296620405	30.016789192210823	20.75971572697279	18.918295784195983
8	27.990443888054134	32.86969032124338	19.2955216528846	19.844344137817888
9	27.71422747079042	14.1781714416407	18.55284802223175	39.55475306533713
10	16.432545747014753	26.529883076617043	30.717702617148124	26.319868559220076
11	37.421673804966055	21.466006240082507	21.974064943987685	19.138255010963753
12	24.639800986649547	23.64492553451169	28.959357904942202	22.755915573896562
13	29.216047155840673	20.26200749740529	25.095840772525786	25.426104574228255
14	23.485795039377248	19.77603974986476	24.846496446368825	31.891668764389163
15	25.978108067270927	26.91518071459583	21.67718292941831	25.429528288714927
16	25.957950440689142	25.982585118741824	23.553085627310217	24.506378813258813
17	23.91619547503003	25.569867709281773	25.201879155604185	25.312057660084008
18	24.490907769237538	25.64169762450407	25.946238574931996	23.921156031326397
19	26.033206463767183	24.65099391362769	24.856870009962858	24.45892961264227
20	26.00950041526539	24.480918258708616	24.704905257898744	24.804676068127243
21	26.850280037937786	23.821990830809185	24.391363682796346	24.936365448456684
22	25.761080657042545	24.611428407427603	24.87497249567106	24.752518439858797
23	24.785366917540642	24.646321258986887	25.23449128190608	25.33382054156639
24	24.938333388811284	25.126996680122936	24.70031385215547	25.23435607891031
25	24.968034188688197	24.87747395484614	24.54503716910316	25.609454687362504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	501.5
3	817.0
4	817.0
5	817.0
6	1959.0
7	3101.0
8	3101.0
9	3101.0
10	3592.0
11	4083.0
12	4083.0
13	4083.0
14	5230.5
15	6378.0
16	6378.0
17	6378.0
18	10298.0
19	14218.0
20	14218.0
21	14218.0
22	23778.0
23	33338.0
24	33338.0
25	33338.0
26	51837.0
27	70336.0
28	70336.0
29	70336.0
30	90948.0
31	111560.0
32	111560.0
33	111560.0
34	137613.5
35	163667.0
36	163667.0
37	163667.0
38	191086.5
39	218506.0
40	218506.0
41	218506.0
42	253958.0
43	289410.0
44	289410.0
45	289410.0
46	317002.5
47	344595.0
48	344595.0
49	344595.0
50	347436.0
51	350277.0
52	350277.0
53	350277.0
54	327213.5
55	304150.0
56	304150.0
57	304150.0
58	273763.5
59	243377.0
60	243377.0
61	243377.0
62	214374.5
63	185372.0
64	185372.0
65	185372.0
66	155523.0
67	125674.0
68	125674.0
69	125674.0
70	96993.5
71	68313.0
72	68313.0
73	68313.0
74	54465.5
75	40618.0
76	40618.0
77	40618.0
78	32092.0
79	23566.0
80	23566.0
81	23566.0
82	16561.0
83	9556.0
84	9556.0
85	9556.0
86	6639.5
87	3723.0
88	3723.0
89	3723.0
90	2418.5
91	1114.0
92	1114.0
93	1114.0
94	651.0
95	188.0
96	188.0
97	188.0
98	589.5
99	991.0
100	991.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008253366112442944
2	0.0031332223204644503
3	0.004241313141116512
4	0.013717400159106557
5	0.03240210399699822
6	0.04344480217522049
7	0.06652365926742206
8	0.08570509347319222
9	0.09456982003840872
10	0.11497397514972599
11	0.11638774619676484
12	0.1306782967803466
13	0.12009411894170448
14	0.12104936964916317
15	0.11115297231989131
16	0.1113058124330847
17	0.12127862981895325
18	0.12777433462967222
19	0.13014335638416974
20	0.11932991837573755
21	0.1284239051107441
22	0.1485988000522713
23	0.1369447414212755
24	0.13232132799717553
25	0.1293027357616061
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2617114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.503926546938814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89232339579448	41.147683558412815
2	12.375603989756359	12.747843977248289
3	3.440231701450992	5.315563225679468
4	1.364469183150791	2.811020823382398
5	0.6926910685263556	1.783815495655099
6	0.3940796339337256	1.2177989111860288
7	0.2752167531073014	0.9922320405567816
8	0.19255454675517694	0.7933852185886183
9	0.1553841558333949	0.7202604733741139
>10	0.9648641111266348	9.735669074750174
>50	0.12901940320871688	4.669756213731935
>100	0.10863769104662757	11.700131551985605
>500	0.011657340848536531	4.045502591734254
>1k	0.0032670254607354374	2.3193368437146162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3506	0.13396435921400443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.821002829834696E-5	0.0
9	0.0	0.0	0.0	3.821002829834696E-5	0.0
10	0.0	0.0	0.0	3.821002829834696E-5	0.0
11	0.0	0.0	0.0	3.821002829834696E-5	0.0
12	0.0	0.0	0.0	3.821002829834696E-5	0.0
13	0.0	0.0	0.0	3.821002829834696E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCG	50	8.733976E-5	15.196649	5
GTATCAA	3545	0.0	12.69474	1
ACCGTTA	100	1.4433499E-7	12.3503475	8
CCTAACG	55	0.0030819753	12.084314	2
AACCGCG	215	0.0	11.93057	7
CGACCAT	245	0.0	11.6332035	10
GCGAAAT	115	7.066592E-8	11.566871	12
ACCGTCG	345	0.0	11.565543	8
GACGGTT	75	2.0743033E-4	11.400322	7
AGAACCG	375	0.0	11.144209	5
GTATTAT	145	1.2314558E-9	11.131217	1
GCGTTAT	145	1.2314558E-9	11.131217	1
TACCGTC	380	0.0	11.000311	7
ACGGTAT	330	0.0	10.939911	9
CCGTCGT	310	0.0	10.726315	9
TTCGGAA	240	0.0	10.690664	18
CGAAATT	125	2.2363201E-7	10.64193	13
ATTCGGC	375	0.0	10.640911	11
CGTCGTA	335	0.0	10.493033	10
GTTTTCG	240	0.0	10.293532	15
>>END_MODULE