##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2617114
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233144219166608	32.0	32.0	32.0	32.0	32.0
2	31.21545221186391	32.0	32.0	32.0	32.0	32.0
3	31.376563267782757	32.0	32.0	32.0	32.0	32.0
4	31.52854862264311	32.0	32.0	32.0	32.0	32.0
5	31.41360636181687	32.0	32.0	32.0	32.0	32.0
6	35.01361499728327	36.0	36.0	36.0	36.0	36.0
7	35.04946364583277	36.0	36.0	36.0	36.0	36.0
8	34.96489530070146	36.0	36.0	36.0	36.0	36.0
9	35.15388057226395	36.0	36.0	36.0	36.0	36.0
10	34.97230269678738	36.0	36.0	36.0	36.0	36.0
11	35.148109711690054	36.0	36.0	36.0	36.0	36.0
12	35.05719162405612	36.0	36.0	36.0	36.0	36.0
13	35.103661896272	36.0	36.0	36.0	36.0	36.0
14	35.06002757235642	36.0	36.0	36.0	36.0	36.0
15	35.00527718700828	36.0	36.0	36.0	36.0	36.0
16	35.02681579785978	36.0	36.0	36.0	36.0	36.0
17	34.99475796621775	36.0	36.0	36.0	36.0	36.0
18	35.00892624471078	36.0	36.0	36.0	36.0	36.0
19	35.00061327095419	36.0	36.0	36.0	36.0	36.0
20	34.99370642623898	36.0	36.0	36.0	36.0	36.0
21	34.990847551921696	36.0	36.0	36.0	36.0	36.0
22	34.97244292759123	36.0	36.0	36.0	32.0	36.0
23	34.92880707527452	36.0	36.0	36.0	32.0	36.0
24	34.91082276125534	36.0	36.0	36.0	32.0	36.0
25	34.87224515248476	36.0	36.0	36.0	32.0	36.0
26	34.82933911170855	36.0	36.0	36.0	32.0	36.0
27	34.81023409755937	36.0	36.0	36.0	32.0	36.0
28	34.79291043492947	36.0	36.0	36.0	32.0	36.0
29	34.77503769419292	36.0	36.0	36.0	32.0	36.0
30	34.76008802062119	36.0	36.0	36.0	32.0	36.0
31	34.75372910771178	36.0	36.0	36.0	32.0	36.0
32	34.7103102883558	36.0	36.0	36.0	32.0	36.0
33	34.6993906264687	36.0	36.0	36.0	32.0	36.0
34	34.68455596508215	36.0	36.0	36.0	32.0	36.0
35	34.67235932404932	36.0	36.0	36.0	32.0	36.0
36	34.63372057923346	36.0	36.0	36.0	32.0	36.0
37	34.626968867233145	36.0	36.0	36.0	32.0	36.0
38	34.59940491701928	36.0	36.0	36.0	32.0	36.0
39	34.59383886219706	36.0	36.0	36.0	32.0	36.0
40	34.592729242975274	36.0	36.0	36.0	32.0	36.0
41	34.55491201376784	36.0	36.0	36.0	32.0	36.0
42	34.540703232644816	36.0	36.0	36.0	32.0	36.0
43	34.52294282939146	36.0	36.0	36.0	32.0	36.0
44	34.493326618557695	36.0	36.0	36.0	32.0	36.0
45	34.457522675741295	36.0	36.0	36.0	32.0	36.0
46	34.45259511049194	36.0	36.0	36.0	32.0	36.0
47	34.4355977615037	36.0	36.0	36.0	32.0	36.0
48	34.39912361479095	36.0	36.0	36.0	32.0	36.0
49	34.374384914069466	36.0	36.0	36.0	32.0	36.0
50	33.837561527698064	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	56.0
22	202.0
23	610.0
24	1476.0
25	3540.0
26	7531.0
27	13864.0
28	24370.0
29	39047.0
30	59227.0
31	88717.0
32	138422.0
33	240254.0
34	534822.0
35	1464961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84992364568541	18.198580833920577	11.708616955090822	26.24287856530319
2	16.866646720246877	20.076491845085865	36.99671113342664	26.060150301240615
3	18.941865739023047	24.162153714592925	28.20919515019887	28.68678539618516
4	12.754048925648634	15.857276373898882	36.225705108757204	35.16296959169528
5	14.956666006906843	36.22887654110596	33.05946932384298	15.754988128144207
6	33.72037662975821	35.9462322122222	16.688204451787588	13.645186706231998
7	30.301148747168632	30.072652603327636	20.677105144907824	18.949093504595908
8	28.054910867466987	32.967383155643965	19.158966709130745	19.8187392677583
9	27.761419640107388	13.932751878596042	18.3995041866728	39.90632429462377
10	16.369711063408012	26.57048947810451	30.69594981342043	26.363849645067045
11	37.86770465482206	21.253220150134844	21.859727929314506	19.019347265728584
12	24.71405525322932	23.490952247399235	28.885291202446666	22.90970129692478
13	29.448048499224715	20.046776716642835	25.14258072059528	25.362594063537163
14	23.530423206631426	19.692837224515248	24.782221943713573	31.99451762513975
15	26.152815658775275	26.83688979540058	21.605210930819215	25.405083615004926
16	26.090342262507484	25.91786219476874	23.47436909511775	24.517426447606027
17	24.132001892160602	25.45124132918933	25.08304949650646	25.333707282143614
18	24.770415044969383	25.496176322468184	25.847708582812977	23.885700049749456
19	26.29312288268681	24.576346311242077	24.730485565397608	24.400045240673503
20	26.280933883659635	24.337648264462306	24.578753543024874	24.80266430885319
21	27.10760784589437	23.811343334680874	24.206587867398973	24.874460952025782
22	25.96363780867016	24.525030243237396	24.785202325920842	24.726129622171598
23	24.973501345375094	24.503403367220532	25.12897795052107	25.394117336883298
24	25.153470578660308	24.979997050185816	24.559228218564417	25.307304152589456
25	25.267298252961083	24.56709948439388	24.499582364390697	25.666019898254337
26	24.771905236073017	25.123590336531006	25.115948330871333	24.988556096524643
27	25.69341648854425	24.72559468177542	24.65093228648045	24.93005654319988
28	24.739579552132614	24.968381201583117	25.215332614475333	25.076706631808932
29	24.988976406835928	24.528278095642758	24.743973705386928	25.73877179213439
30	24.757882155687525	25.027186435134276	25.219803187786237	24.99512822139196
31	25.58184320591308	24.472644294440364	24.365083064780517	25.58042943486604
32	25.07995448421429	24.794219892599255	24.46672174005412	25.659103883132335
33	25.026078344313625	24.337953944688692	24.967005640564377	25.66896207043331
34	25.115222340333666	24.403751613418446	25.428162472097128	25.052863574150763
35	25.599572659043513	24.213007152153097	25.097378257118336	25.090041931685054
36	25.142809980765072	24.81997345167234	24.58249812579811	25.454718441764477
37	25.70594937782611	24.76934516417703	24.164251156044404	25.360454301952455
38	25.06520541329113	24.690556085825836	24.942054492085557	25.30218400879748
39	25.82845072855061	23.768509892958427	24.75451967319727	25.648519705293694
40	25.635566505700552	24.47321744486484	24.9133205508052	24.977895498629408
41	25.13226401295473	24.770835355280667	25.440351471124302	24.65654916064031
42	25.760513298236148	25.294236322911424	24.666178087771492	24.27907229108094
43	25.087061549477784	24.228978943981804	25.121526995002892	25.56243251153752
44	24.816343498983993	24.217133835209317	25.255032833877316	25.71148983192937
45	25.342151698397547	24.52930976640681	24.93544415718994	25.193094378005696
46	25.308508004242846	24.165606974405378	24.770701444951534	25.75518357640025
47	24.777254640034787	24.31972776119038	25.302451478995565	25.600566119779266
48	25.04480125817981	24.863074363592872	24.803619559560648	25.288504818666667
49	24.792844331580515	24.604086791786678	25.30184011854279	25.301228758090016
50	24.723473813883395	25.049864125035537	24.91142908671849	25.31523297436258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	13.5
2	18.0
3	51.0
4	84.0
5	119.0
6	154.0
7	160.0
8	166.0
9	237.0
10	308.0
11	463.0
12	618.0
13	1105.0
14	1592.0
15	2261.5
16	2931.0
17	3378.0
18	3825.0
19	4422.5
20	5020.0
21	5937.5
22	6855.0
23	7985.0
24	9115.0
25	11333.5
26	13552.0
27	17680.5
28	21809.0
29	26543.5
30	31278.0
31	35882.5
32	40487.0
33	48484.0
34	56481.0
35	65094.5
36	73708.0
37	87043.0
38	100378.0
39	108993.0
40	117608.0
41	128237.0
42	138866.0
43	142990.5
44	147115.0
45	158772.0
46	170429.0
47	179948.5
48	189468.0
49	196900.5
50	204333.0
51	201029.0
52	197725.0
53	190423.5
54	183122.0
55	182314.5
56	181507.0
57	173261.0
58	165015.0
59	151327.5
60	137640.0
61	123848.5
62	110057.0
63	98299.0
64	86541.0
65	73759.5
66	60978.0
67	54589.0
68	48200.0
69	43008.5
70	37817.0
71	31212.0
72	24607.0
73	22151.5
74	19696.0
75	15038.0
76	10380.0
77	8830.0
78	7280.0
79	5832.0
80	4384.0
81	3502.5
82	2621.0
83	2170.5
84	1720.0
85	1324.5
86	929.0
87	693.5
88	458.0
89	294.0
90	130.0
91	89.5
92	49.0
93	31.5
94	14.0
95	16.0
96	18.0
97	14.5
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013984870357194987
2	0.003935632914729736
3	6.495704810718983E-4
4	0.0
5	0.0
6	3.0568022638677566E-4
7	7.642005659669391E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.642005659669391E-5
47	0.0
48	0.0
49	0.0
50	7.642005659669391E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2617114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.54795917215727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53899301421768	44.73776695720423
2	12.313061509273659	13.679308760027887
3	3.2883561287012695	5.479844159418336
4	1.2293911513297555	2.731606779227067
5	0.6277738593934791	1.7435778355468285
6	0.36642512692206997	1.2212500793951813
7	0.24634465729887178	0.9578760076141761
8	0.18026279761947472	0.8010584417940343
9	0.13390849963551815	0.6694509483502737
>10	0.8660038875266689	9.254660583879652
>50	0.10898034198949061	4.24172191767394
>100	0.09096398343629453	10.25628956777922
>500	0.007738585343960591	2.896276181869455
>1k	0.0017964573119885422	1.3293117802198413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.821002829834696E-5
6	0.0	0.0	0.0	0.0	3.821002829834696E-5
7	0.0	0.0	0.0	3.821002829834696E-5	3.821002829834696E-5
8	0.0	0.0	0.0	3.821002829834696E-5	3.821002829834696E-5
9	0.0	0.0	0.0	3.821002829834696E-5	3.821002829834696E-5
10	0.0	0.0	0.0	3.821002829834696E-5	3.821002829834696E-5
11	0.0	0.0	0.0	3.821002829834696E-5	3.821002829834696E-5
12	0.0	0.0	0.0	3.821002829834696E-5	7.642005659669391E-5
13	0.0	0.0	0.0	3.821002829834696E-5	1.5284011319338783E-4
14	0.0	0.0	0.0	3.821002829834696E-5	1.910501414917348E-4
15	0.0	0.0	0.0	1.1463008489504087E-4	1.910501414917348E-4
16	0.0	0.0	0.0	1.1463008489504087E-4	1.910501414917348E-4
17	0.0	0.0	0.0	1.1463008489504087E-4	1.910501414917348E-4
18	0.0	0.0	0.0	1.910501414917348E-4	1.910501414917348E-4
19	0.0	0.0	0.0	2.2926016979008174E-4	1.910501414917348E-4
20	0.0	0.0	0.0	3.438902546851226E-4	1.910501414917348E-4
21	0.0	0.0	0.0	3.821002829834696E-4	1.910501414917348E-4
22	0.0	0.0	0.0	4.967303678785104E-4	1.910501414917348E-4
23	0.0	0.0	0.0	9.552507074586739E-4	1.910501414917348E-4
24	0.0	0.0	0.0	0.0016048211885305722	1.910501414917348E-4
25	0.0	0.0	0.0	0.001795871330022307	1.910501414917348E-4
26	0.0	0.0	0.0	0.0021779716130057767	1.910501414917348E-4
27	0.0	0.0	0.0	0.0029039621506743688	1.910501414917348E-4
28	0.0	0.0	0.0	0.0055404541032603085	1.910501414917348E-4
29	0.0	0.0	0.0	0.0106605978952388	1.910501414917348E-4
30	0.0	0.0	0.0	0.020748045366002398	1.910501414917348E-4
31	0.0	0.0	0.0	0.04676907463717667	1.910501414917348E-4
32	0.0	0.0	0.0	0.0811198900773906	1.910501414917348E-4
33	0.0	0.0	0.0	0.12039979916809126	1.910501414917348E-4
34	0.0	0.0	0.0	0.16709245374867124	1.910501414917348E-4
35	0.0	0.0	0.0	0.21527529943288676	1.910501414917348E-4
36	0.0	0.0	0.0	0.28290704952096085	1.910501414917348E-4
37	0.0	0.0	0.0	0.3863415961245861	1.910501414917348E-4
38	0.0	0.0	0.0	0.5299730924980723	1.910501414917348E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3965	0.0	24.52915	1
GGACCGT	325	0.0	18.953749	6
AACGCGT	70	8.127064E-4	18.857044	6
TTAGGAC	765	0.0	18.692713	3
ACCGTTA	220	0.0	17.999907	8
CCGTTTA	245	0.0	17.959091	27
CGCAATA	490	0.0	17.510115	36
TAGGACG	705	0.0	17.475088	4
ATACGAA	535	0.0	17.270937	40
GGTATCA	1930	0.0	17.101625	1
TAGGACC	1290	0.0	17.054174	4
ATCAACG	5735	0.0	16.763645	3
CTAGCGG	540	0.0	16.703617	29
AACGCAG	5770	0.0	16.700087	6
GTCCTAC	995	0.0	16.585997	1
TTATTCG	315	0.0	16.063408	22
TATCAAC	6150	0.0	16.062319	2
TCAACGC	6065	0.0	15.887797	4
CAACGCA	6105	0.0	15.747665	5
CCGTTAT	240	3.6379788E-12	15.583253	9
>>END_MODULE