FastQCFastQC Report
Thu 2 Feb 2017
SRR4062378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606493
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG75671.247664853510263No Hit
TCCATGTACTCTGCGTTGATACCAC59400.9794012461809122No Hit
CCATAGGGTCTTCTCGTCTTATTAT31700.5226770960258403No Hit
GTATTAGAGGCACTGCCTGCCCAGT29680.4893708583611022No Hit
GAGTACATGGAAGCAGTGGTATCAA29400.4847541521501485No Hit
GTATCAACGCAGAGTACTTTTTTTT23620.389452145366888No Hit
GTCAGGATACCGCGGCCGTTAAACT22220.366368614312119No Hit
CTCTAATACTTGTAATGCTAGAGGT22210.3662037319474421No Hit
CCATTGGGATGTCCTGATCCAACAT21920.3614221433718114No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21600.3561459077021499No Hit
CATGTACTCTGCGTTGATACCACTG21540.35515661351408834No Hit
GCTTCCATGTACTCTGCGTTGATAC19260.3175634343677503No Hit
ATCTATAACTTTATAGATGCAACAC18790.30981396322793503No Hit
CTTCTACACCATTGGGATGTCCTGA18730.3088246690398735No Hit
CTGTTAGTATGAGTAACAAGAATTC17790.29332572676024293No Hit
GATATATTTTGATCAACGGACCAAG17640.29085249129008905No Hit
CATGGAAGCAGTGGTATCAACGCAG16940.2793107257627046No Hit
GTTATATAATTTAAGCTCCATAGGG16510.272220784081597No Hit
TATCAACGCAGAGTACTTTTTTTTT16200.2671094307766124No Hit
GTATCCTGACCGTGCAAAGGTAGCA16160.26644990131790475No Hit
ATAAATAATCCACCTATAACTTCTC16100.2654606071298432No Hit
GCGTTGATACCACTGCTTCCATGTA15840.26117366564824324No Hit
CCTCTAGCATTACAAGTATTAGAGG15780.26018437146018175No Hit
ACCTATAACTTCTCTGTTAACCCAA15510.2557325476139049No Hit
CACTATAAATAATCCACCTATAACT15430.2544134886964895No Hit
GTACATGGGAGAAATCGTAAATAGA15390.25375395923778177No Hit
GACTATAGGCAATAATCACACTATA14540.2397389582402435No Hit
ATCCTGACCGTGCAAAGGTAGCATA14400.23743060513476663No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14140.23314366365316663No Hit
GTTCATGCTAGTCCCTAATTAAGGA13940.22984601635962823No Hit
GGTCAGGATACCGCGGCCGTTAAAC13850.22836207507753595No Hit
ATCGTAAATAGATAGAAACCGACCT13780.22720789852479747No Hit
CCCATGTACTCTGCGTTGATACCAC13550.2234156041372283No Hit
CCTATAACTTCTCTGTTAACCCAAC13540.22325072177255137No Hit
TGCTAGAGGTGATGTTTTTGGTAAA13400.22094236866707448No Hit
GCCTAAAGGAAAGATCCAAAAAGAT13310.2194584273849822No Hit
GTATCAACGCAGAGTACATGGAAGC12720.20973036786904384No Hit
CTATAGAACTAGTACCGCAAGGGAA12570.20725713239889No Hit
GATAGAAACCGACCTGGATTGCTCC12520.2064327205755054No Hit
CTGTTAACCCAACACCGGAATGCCT12430.20494877929341312No Hit
GTATCAACGCAGAGTACATGGGAGA12390.20428924983470542No Hit
GTTATAGATTAACCCAATTTTAAGT12240.20181601436455163No Hit
GATTAAAGATAAGAGACAGTTGGAC12200.20115648490584395No Hit
GATTAAACCTTGTACCTTTTGCATA10980.18104083641525953No Hit
ACCTTTGCACGGTCAGGATACCGCG10380.17114789453464427No Hit
CCATTAATAGCTTCTACACCATTGG10380.17114789453464427No Hit
GTTAGTATGAGTAACAAGAATTCCA10280.16949907088787503No Hit
AGGGATAACAGCGCAATCCTATTTA9960.16422283521821357No Hit
GACCTGGATTGCTCCGGTCTGAACT9830.16207936447741358No Hit
GTCCTGATCCAACATCGAGGTCGTA9710.16010077610129053No Hit
TCCCAATGGTGTAGAAGCTATTAAT9700.1599358937366136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9670.15944124664258286No Hit
ACGCAGAGTACATGGAAGCAGTGGT9430.15548406989033672No Hit
GGATTGCTCCGGTCTGAACTCAGAT9410.15515430516098289No Hit
CCTATAGTCTGATTAACTAACAATG9210.15185665786744448No Hit
AAATATATCTGGGTCAATAAGATAT9160.15103224604405985No Hit
GGATACCGCGGCCGTTAAACTTTAG9090.1498780694913214No Hit
CAGTTGGACCCTCGTTTAGCCGTTC8980.14806436347987528No Hit
CAGTGGTATCAACGCAGAGTACATG8970.14789948111519838No Hit
ATCCTAGCCCTAGCCCTACACAAAT8940.1474048340211676No Hit
CTTAAATAGGATTGCGCTGTTATCC8820.14542624564504453No Hit
GGTATCAACGCAGAGTACTTTTTTT8790.14493159855101378No Hit
GTACATGGGGTGGTATCAACGCAAA8690.14328277490424457No Hit
ATTATAACCTAGACTTACAAGTCAA8650.1426232454455369No Hit
TACTAACAGTGTTGCATCTATAAAG8580.14146906889279842No Hit
GTTATAGGTGGATTATTTATAGTGT8440.13916071578732153No Hit
CCTTTAGGCATTCCGGTGTTGGGTT8400.13850118632861386No Hit
GTGTAGAAGCTATTAATGGTTCGTT8360.13784165686990618No Hit
CTTTATTGGTGGCTGCTTTTAGGCC8290.1366874803171677No Hit
ACTCTGCGTTGATACCACTGCTTCC8260.13619283322313694No Hit
ATTTAAGAGTTCATATCGACAATTA8170.13470889194104466No Hit
GTACATGGGAAGCAGTGGTATCAAC8130.13404936248233698No Hit
CTATTAAAGGTTTTTTCCGTTCCAG8080.13322495065895237No Hit
AGCATGAACGGCTAAACGAGGGTCC8030.13240053883556777No Hit
ACCCTATGGAGCTTAAATTATATAA7900.13025706809476778No Hit
ATGCAACACTGTTAGTATGAGTAAC7820.12893800917735243No Hit
CTATAGTATAAGTTTGAAATTTCGG7810.1287731268126755No Hit
TATCAACGCAGAGTACATGGGAGAA7730.12745406789526012No Hit
CTCTTAAATAGGATTGCGCTGTTAT7700.12695942080122935No Hit
ATTTCAGCCTCTTCACTGAAAGGTC7610.12547547951913707No Hit
TTATATAACTTATCTATTTAATTTA7520.12399153823704478No Hit
GGGCAGGCAGTGCCTCTAATACTTG7360.12135342040221404No Hit
GTTATACGCGTATGCCTGGAGAATT7360.12135342040221404No Hit
TATCAACGCAGAGTACATGGAAGCA7340.12102365567286019No Hit
GATAAAAGGAACTCGGCAAACAAGA7250.11953971439076791No Hit
CTATTATATAAATCAAAACATTTAT7250.11953971439076791No Hit
CCAATAAAGAAAGCGTTCAAGCTCA7230.11920994966141407No Hit
GCTATCACCAAGCTCGTTAGGCTTT7170.11822065547335253No Hit
GTATAAGTTTGAAATTTCGGTTGGG7130.11756112601464484No Hit
GTCCTACAGTGGACATTTCTAAATT7100.11706647892061409No Hit
ACTAAAAGTATTGGAGAAAGAAATT7020.11574742000319872No Hit
GTTTAAAATTGAACTTAAATTCATT7000.11541765527384489No Hit
GTCCTAAAGTGTGTATTTCTCATTT6870.11327418453304489No Hit
CTATAAATAATCCACCTATAACTTC6820.11244977270966029No Hit
GTTATCCCTAGGGTAACTTGGTCCG6780.1117902432509526No Hit
GGGATGTCCTGATCCAACATCGAGG6680.1101414196041834No Hit
TCTAGGAGCTATAGAACTAGTACCG6620.10915212541612186No Hit
CCCTAATTGTCGATATGAACTCTTA6610.10898724305144494No Hit
CATTTATCCTACTAAAAGTATTGGA6600.10882236068676801No Hit
GGTCTGAACTCAGATCACGTAGGAC6500.10717353703999881No Hit
GTTTAGATTATAGCCAAAAGAGGGA6500.10717353703999881No Hit
TAATCACACTATAAATAATCCACCT6450.10634912521661422No Hit
TGCTAGTCCCTAATTAAGGAACAAG6430.10601936048726036No Hit
ACGTAGGACTTTAATCGTTGAACAA6400.10552471339322961No Hit
ACTTTATAGATGCAACACTGTTAGT6380.10519494866387577No Hit
GTGCAAAGGTAGCATAATCACTTGT6260.1032163602877527No Hit
GGTTATAATCATTCGGAGGATTTTT6230.10272171319372193No Hit
CAATTAGGGTTTACGACCTCGATGT6170.10173241900566041No Hit
GGTTTAATCTTTGCTTGTTCTTACT6080.10024847772356812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT359.912987E-519.0034716
AACCTCG359.912987E-519.003479
TAACCTC359.9192745E-519.00198
CTCTAGA401.2911492E-518.986221
TGAACTT1000.018.05329710
AACTTGC453.5301047E-516.8905777
GGGCCGA402.7555233E-416.62666115
CGCGCTA350.002165499416.28868916
CGATCGC350.002165499416.28868919
GCGCGCT350.002166694716.28734215
GTTTTAT350.002178678316.2739031
TTGAACT1150.015.6985189
ATTGAAC1150.015.6972218
TCTAGAT551.1320397E-515.5367432
TCTTACA508.741649E-515.19148352
GAACTTA1200.015.04441411
TAAATTC1200.015.04441416
CATTCCG1400.014.9312979
CCTCGGG456.744556E-414.78047611
AGTAAAT456.748782E-414.77925513
Produced by FastQC (version 0.11.5)