FastQCFastQC Report
Thu 2 Feb 2017
SRR4062378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606493
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG33080.5454308623512555No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT28870.4760153868222716No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23060.38021873294498043No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21610.3563107900668268No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC21170.34905596602104233No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT20970.34575831872750384No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA20550.3388332594110732No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC18780.3096490808632581No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA18110.2986019624299044No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC17110.28211372596221224No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA16980.2799702552214123No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA16550.2728803135403047No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC16480.2717261369875662No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC16060.26480107767113553No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA15580.2568867241666433No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG15370.25342419450842796No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC15190.2504563119442434No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT14740.24303660553378192No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT14590.2405633700636281No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT14310.2359466638526743No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC13830.2280323103481821No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG13810.22770254561882822No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC13590.22407513359593598No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT13540.22325072177255137No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC12460.20544342638744387No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC12340.20346483801132081No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT12330.2032999556466439No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT12140.2001671907177824No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA11950.19703442578892089No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG11900.19621001396553628No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA11820.19489095504812093No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC11730.19340701376602865No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11130.18351407188541335No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA11130.18351407188541335No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC10940.18038130695655186No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT10200.16818001197045968No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC9990.1647174823122443No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT9800.16158471738338284No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT9700.1599358937366136No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA9480.15630848171372136No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA9420.15531918752565982No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA9350.15416501097292137No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT9210.15185665786744448No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA8920.14707506929181377No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8920.14707506929181377No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8510.14031489234005998No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC8370.1380065392345831No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA8330.1373470097758754No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG8240.1358630684937831No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT8220.13553330376442926No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA8180.1348737743057216No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATC8030.13240053883556777No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA7990.13174100937686006No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7960.13124636228282932No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT7950.1310814799181524No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7920.13058683282412162No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.1295975386360601No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT7840.12926777390670627No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAA7840.12926777390670627No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC7800.1286082444479986No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT7600.12531059715446016No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC7550.12448618533107556No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT7320.12069389094350635No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC7260.11970459675544483No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA7230.11920994966141407No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT7160.11805577310867561No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7140.11772600837932176No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA7050.11624206709722948No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC7010.1155825376385218No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT7000.11541765527384489No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT6920.11409859635642952No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG6870.11327418453304489No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA6770.11162536088627568No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6770.11162536088627568No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC6760.11146047852159877No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT6750.11129559615692185No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC6730.11096583142756801No Hit
TAATCACACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACC6690.11030630196886032No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA6630.1093170077807988No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT6530.10766818413402958No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA6480.10684377231064497No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG6420.10585447812258345No Hit
CGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTA6340.10453541920516807No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTCA6270.10338124265242962No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC6190.10206218373501426No Hit
GATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATT6160.10156753664098349No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG6110.1007431248175989No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6090.10041336008824506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCT357.2847997E-637.71407343
TTATCTG250.002351936435.199835
GAAAGCC250.002351936435.199833
CGAGAAT353.216092E-431.42839621
CATTGTG300.00574294329.333177
AATGGCG300.00574294329.3331716
TCAGAGC300.00574294329.333173
CGCTTTA300.00574294329.3331713
CTGTGCG300.00574294329.3331738
CCCGTTT407.0282945E-427.49984622
GGCTCGG508.335021E-526.3998529
CTGTGCA609.742947E-625.6665239
ACTTGCT1550.025.5482438
CTGGCGA701.1307693E-625.14271529
GTCCTAT1151.2732926E-1124.8735261
GTATTAT801.3055615E-724.7539421
AAGGACC1251.8189894E-1224.639864
GGATAGA450.001396115324.4483381
GGACTGT901.5095793E-824.44430536
TAGGGAG450.001397457324.4443055
Produced by FastQC (version 0.11.5)