FastQCFastQC Report
Thu 2 Feb 2017
SRR4062377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949083
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG180711.904048434120093No Hit
TCCATGTACTCTGCGTTGATACCAC148651.5662486842562768No Hit
GAGTACATGGAAGCAGTGGTATCAA69980.7373433092785352No Hit
CATGTACTCTGCGTTGATACCACTG58040.6115376631970018No Hit
CCCATGTACTCTGCGTTGATACCAC46620.4912109899766406No Hit
GTACATGGGAAGCAGTGGTATCAAC43960.46318393649448997No Hit
GCGTTGATACCACTGCTTCCATGTA42800.4509616124195671No Hit
GCTTCCATGTACTCTGCGTTGATAC42630.44917040975341455No Hit
GTATCAACGCAGAGTACTTTTTTTT40690.4287296263867333No Hit
CATGGAAGCAGTGGTATCAACGCAG37560.39575042435698454No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36270.38215835706676865No Hit
GTATCAACGCAGAGTACATGGAAGC31940.3365353715112377No Hit
TATCAACGCAGAGTACTTTTTTTTT28550.3008166830509028No Hit
CAGTGGTATCAACGCAGAGTACATG26840.2827992915266631No Hit
ACTCTGCGTTGATACCACTGCTTCC24880.2621477784345521No Hit
ACGCAGAGTACATGGAAGCAGTGGT24290.25593125153437585No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21600.2275881034640806No Hit
GTATCAACGCAGAGTACATGGGAAG20330.21420676589929435No Hit
TATCAACGCAGAGTACATGGAAGCA18670.1967161986886289No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16190.1705857127353456No Hit
GTACATGGGGTGGTATCAACGCAAA14670.15457025360268806No Hit
GAGTACATGGGAAGCAGTGGTATCA14100.1485644564279415No Hit
ATACCACTGCTTCCATGTACTCTGC13930.146773253761789No Hit
GCGTTGATACCACTGCTTCCCATGT13830.1457196051346405No Hit
GGTATCAACGCAGAGTACATGGAAG13780.14519278082106624No Hit
GGTATCAACGCAGAGTACTTTTTTT12810.13497238913772558No Hit
GTACTCTGCGTTGATACCACTGCTT12690.13370801078514735No Hit
AAAAAGTACTCTGCGTTGATACCAC12500.13170607839356516No Hit
AAGCAGTGGTATCAACGCAGAGTAC12400.13065242976641664No Hit
GTGGTATCAACGCAGAGTACATGGA11700.12327688937637697No Hit
TATCAACGCAGAGTACATGGGAAGC11300.11906229486778291No Hit
GAAGCAGTGGTATCAACGCAGAGTA11070.1166389030253413No Hit
CCACTGCTTCCATGTACTCTGCGTT10950.11537452467276307No Hit
ACATGGAAGCAGTGGTATCAACGCA10300.1085258085962977No Hit
GGTATCAACGCAGAGTACATGGGAA10030.10568095730299669No Hit
GCTTCCCATGTACTCTGCGTTGATA9660.10178245738254715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA456.752337E-414.77966615
TCGCGTA602.5643581E-514.2510689
TAATACC551.962022E-413.8155724
TTAGCCG655.4415766E-513.15552716
CGCAAGA1301.4551915E-1113.1465062
AGAACTA801.9965555E-613.062795
ATTACAC957.4185664E-812.9941173
CCGTCAA751.4736004E-512.66962318
CGCCAGT1051.9832441E-812.66962318
AAGCTCG604.084999E-412.6682867
AATACAG1457.2759576E-1212.4485535
AAAAAGT2850.012.3238511
TTTGAAC1551.8189894E-1212.2525163
CGTTTAG701.0881975E-412.21649213
CGCGTAA701.0892707E-412.21520110
GAACTAG550.003065202612.0918176
ATAGAGT550.003065202612.0918176
CAGCTTA550.003065202612.0918179
AGTACCG550.003065202612.09181711
TCAGTAT550.003077342212.0854373
Produced by FastQC (version 0.11.5)