FastQCFastQC Report
Thu 2 Feb 2017
SRR4062377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949083
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA66190.6974100263096061No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC58350.6148039739411622No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23270.2451840355374609No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC19790.20851706331269237No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG16410.17290373971507234No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14580.1536219698382544No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA14470.15246295634839105No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14460.15235759148567615No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC13740.14477132137020682No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13210.13918698364631968No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11290.11895693000506805No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10990.11579598412362249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.1078936194200086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA508.3312225E-526.4038891
TCGTATA1200.025.66639144
GTACGAA609.750911E-625.66639119
ACGAATT651.8130097E-523.69205321
TGGTATA4450.022.7413344
GTCGTAT1650.022.66642243
CGGTGAT1850.020.21643
CGATTAT550.004483193619.99978643
TCTATAC901.00122525E-519.5563763
TGTCGTA708.122155E-418.8569442
CTATACT951.5959442E-518.5270924
GTATTAA1203.15531E-718.3360331
ATACCGT2400.018.3341036
ACCGAAC600.007411418518.3331368
TACCGTC2400.018.3331367
TACTCGT1102.7954284E-617.99980736
CTATTCG1002.4823317E-517.5998138
AAGTACT5150.017.5152974
AGTACTC5150.017.5152975
CGAATTT902.2149221E-417.11092822
Produced by FastQC (version 0.11.5)