##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062377_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949083
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064229366662346	32.0	32.0	32.0	32.0	32.0
2	31.403739188248025	32.0	32.0	32.0	32.0	32.0
3	31.50246606461184	32.0	32.0	32.0	32.0	32.0
4	31.61116256428574	32.0	32.0	32.0	32.0	32.0
5	31.561237531385558	32.0	32.0	32.0	32.0	32.0
6	35.14385675436184	36.0	36.0	36.0	36.0	36.0
7	35.158007255424444	36.0	36.0	36.0	36.0	36.0
8	35.112760422428806	36.0	36.0	36.0	36.0	36.0
9	35.206460341192496	36.0	36.0	36.0	36.0	36.0
10	35.11585077385224	36.0	36.0	36.0	36.0	36.0
11	35.21634356531515	36.0	36.0	36.0	36.0	36.0
12	35.12560439919375	36.0	36.0	36.0	36.0	36.0
13	35.14841589197152	36.0	36.0	36.0	36.0	36.0
14	35.098996610412364	36.0	36.0	36.0	36.0	36.0
15	35.07304945932021	36.0	36.0	36.0	36.0	36.0
16	35.05685382627231	36.0	36.0	36.0	36.0	36.0
17	35.04670613634424	36.0	36.0	36.0	36.0	36.0
18	35.025262279484515	36.0	36.0	36.0	36.0	36.0
19	35.00148142996977	36.0	36.0	36.0	36.0	36.0
20	34.97752356748567	36.0	36.0	36.0	36.0	36.0
21	34.92599488137497	36.0	36.0	36.0	32.0	36.0
22	34.89127189086729	36.0	36.0	36.0	32.0	36.0
23	34.79070429035185	36.0	36.0	36.0	32.0	36.0
24	34.76403222900421	36.0	36.0	36.0	32.0	36.0
25	34.72322125672886	36.0	36.0	36.0	32.0	36.0
26	34.641196818402605	36.0	36.0	36.0	32.0	36.0
27	34.58927933594849	36.0	36.0	36.0	32.0	36.0
28	34.52695180505815	36.0	36.0	36.0	32.0	36.0
29	34.46867871408507	36.0	36.0	36.0	32.0	36.0
30	34.433425738317936	36.0	36.0	36.0	32.0	36.0
31	34.40225670462963	36.0	36.0	36.0	32.0	36.0
32	34.337035854609134	36.0	36.0	36.0	32.0	36.0
33	34.2747241284482	36.0	36.0	36.0	32.0	36.0
34	34.219779513488284	36.0	36.0	36.0	32.0	36.0
35	34.09471985063477	36.0	36.0	36.0	32.0	36.0
36	34.0591434047391	36.0	36.0	36.0	32.0	36.0
37	33.94772006241814	36.0	36.0	36.0	32.0	36.0
38	33.85988053731865	36.0	36.0	36.0	32.0	36.0
39	33.64548306101785	36.0	36.0	36.0	27.0	36.0
40	33.4864590346682	36.0	36.0	36.0	21.0	36.0
41	33.517147604582526	36.0	36.0	36.0	21.0	36.0
42	33.262955926931575	36.0	36.0	36.0	14.0	36.0
43	33.332892908207185	36.0	36.0	36.0	14.0	36.0
44	33.33132297175273	36.0	36.0	36.0	14.0	36.0
45	33.045644058528076	36.0	36.0	36.0	14.0	36.0
46	33.23741443056087	36.0	36.0	36.0	14.0	36.0
47	33.07331076417974	36.0	36.0	36.0	14.0	36.0
48	33.11635547154464	36.0	36.0	36.0	14.0	36.0
49	33.17920245120817	36.0	36.0	36.0	14.0	36.0
50	32.56156943070311	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	9.0
21	35.0
22	160.0
23	502.0
24	1362.0
25	3284.0
26	6979.0
27	13286.0
28	21039.0
29	27259.0
30	34596.0
31	44190.0
32	61409.0
33	73857.0
34	144613.0
35	516498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82746628784845	17.369925262088532	12.619241323784191	25.183367126278828
2	14.405805439385652	21.61086823435447	36.36692197702733	27.616404349232543
3	18.934757870780125	26.560880010115056	27.70746407046824	26.796898048636574
4	12.416827611494464	17.04297727385276	34.37296843374078	36.167226680911995
5	13.572680155476391	37.355004778296525	33.556285382837956	15.516029683389124
6	34.00363511840476	33.468271738271476	17.993941469325396	14.534151673998366
7	28.75241549202282	30.956018552664577	22.065848893983873	18.225717061328737
8	29.163729621118488	31.48144050625709	20.834637223509432	18.52019264911499
9	27.405611521858468	15.580302249645184	18.407452246010095	38.60663398248626
10	15.898714864769467	27.557442289030572	31.990879617483404	24.552963228716564
11	36.043844426672905	21.836446338202244	20.824627561551516	21.295081673573332
12	23.60657603181176	24.594266254900784	29.15098047272999	22.648177240557466
13	30.97695354357838	20.127744359555486	24.848195574043576	24.047106522822556
14	25.06440427233445	19.124670866510094	24.976635341692983	30.83428951946247
15	26.477347081340618	25.069040326293905	25.454675723830267	22.998936868535207
16	26.15356085821788	25.162604324384695	25.25932926835693	23.424505549040493
17	24.135507642640317	24.67697767213194	27.64257709810417	23.54493758712357
18	26.116788521130395	22.71329272571524	28.717509427521094	22.45240932563327
19	24.01876337475226	28.35642404299729	25.7954256898501	21.82938689240035
20	24.099894319042697	22.821291709997965	29.793495405565164	23.285318565394174
21	25.17956806728179	25.88066586378641	24.06607219811123	24.87369387082057
22	23.659890652345474	25.88424826911872	25.242576255185266	25.21328482335054
23	22.668828753649574	24.966520314872355	25.289674348818806	27.07497658265926
24	22.634058348953673	27.63520155771413	25.350891334056136	24.37984875927606
25	25.46795169653234	23.773684704077514	24.48826920300964	26.270094396380507
26	22.845525628422383	24.438747717533662	26.462701365423257	26.2530252886207
27	26.078330346239476	25.664773260083678	25.59839339657332	22.65850299710352
28	24.542005282994218	22.994090084850324	26.516121350819688	25.947783281335774
29	22.98292140940255	26.087602454158382	28.026842752425235	22.902633384013832
30	25.95062813262907	24.054482063212596	27.214901120344585	22.779988683813745
31	22.87070783061123	24.893923924461824	26.12922157493075	26.106146669996193
32	22.83836081775777	25.836201891720744	26.288743977081037	25.036693313440445
33	22.81813076411652	25.019729570543358	26.34047812467403	25.821661540666096
34	22.504248838088976	26.275257274653534	27.826544148404302	23.393949738853188
35	25.548871911097343	24.981903584828725	26.42571829861034	23.04350620546359
36	24.146149493774516	25.990034591284427	26.303178963272973	23.560636951668084
37	25.44329631865706	25.76033919056605	26.378198745525943	22.418165745250942
38	23.983044686291926	25.677522408472175	25.041013272811757	25.298419632424142
39	23.44283903515288	25.689007178508096	26.589982119582796	24.278171666756226
40	24.64737014570907	25.66182304392767	25.19589962100259	24.494907189360678
41	22.29151718026769	25.878347836806686	26.849074316998617	24.981060665927004
42	25.911327038836436	25.70702457003234	25.99224725340144	22.389401137729788
43	24.73145130615552	23.700034665039833	25.170612053951025	26.39790197485362
44	24.217586870695186	24.662542685940007	27.69062347550214	23.429246967862664
45	24.99433663862908	25.019097381367068	25.96242899725314	24.02413698275072
46	23.42294310899631	24.82114220327518	27.710999517426387	24.044915170302126
47	24.67481700718906	24.14735939292853	27.819859421903924	23.357964177978484
48	23.653990220033442	28.714137751914215	24.36551913794684	23.2663528901055
49	22.531538337532123	25.461629804769448	28.620995213274288	23.385836644424142
50	22.58195032879175	28.613469240243983	23.830842678338307	24.97373775262596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	50.5
2	57.0
3	219.0
4	381.0
5	408.0
6	435.0
7	402.0
8	369.0
9	483.0
10	597.0
11	924.0
12	1251.0
13	2071.5
14	2892.0
15	3799.5
16	4707.0
17	5387.0
18	6067.0
19	6403.5
20	6740.0
21	7180.5
22	7621.0
23	7570.0
24	7519.0
25	7340.5
26	7162.0
27	7965.0
28	8768.0
29	10064.5
30	11361.0
31	12403.5
32	13446.0
33	15465.0
34	17484.0
35	19607.5
36	21731.0
37	25980.0
38	30229.0
39	33170.0
40	36111.0
41	41699.0
42	47287.0
43	53172.5
44	59058.0
45	70044.0
46	81030.0
47	89502.5
48	97975.0
49	92940.5
50	87906.0
51	76852.5
52	65799.0
53	60971.0
54	56143.0
55	56479.0
56	56815.0
57	54773.0
58	52731.0
59	48434.0
60	44137.0
61	38846.5
62	33556.0
63	29133.5
64	24711.0
65	21025.0
66	17339.0
67	14995.0
68	12651.0
69	11429.5
70	10208.0
71	8026.0
72	5844.0
73	5193.0
74	4542.0
75	3244.0
76	1946.0
77	1786.5
78	1627.0
79	1380.0
80	1133.0
81	896.0
82	659.0
83	565.0
84	471.0
85	396.5
86	322.0
87	232.0
88	142.0
89	90.5
90	39.0
91	26.0
92	13.0
93	15.0
94	17.0
95	12.0
96	7.0
97	4.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017595932073380303
2	0.0044253242340237895
3	3.160945881445564E-4
4	0.0
5	0.0
6	8.429189017188171E-4
7	1.0536486271485214E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.268243135742607E-4
47	2.1072972542970428E-4
48	0.0
49	0.0
50	2.1072972542970428E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	949083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.70739955587931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33819318657743	46.93815613371124
2	12.078643412932252	13.940542030461426
3	3.1219011992376475	5.404703996351571
4	1.1663534200956949	2.6922889134731447
5	0.5843571032593118	1.6860864420550663
6	0.34774769466394956	1.2040569096365075
7	0.2143777279293867	0.8659826841051894
8	0.14695619844698257	0.6784368048794491
9	0.11451663821235819	0.5947611657404969
>10	0.7043809552697945	7.9600255911647055
>50	0.09230067404214078	3.7779970174876896
>100	0.08218226043484306	9.06766443453348
>500	0.005699440814725553	2.1444274023797587
>1k	0.0020223822245800348	1.723541616448604
>5k	3.677058590145518E-4	1.3213288575716167
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6619	0.6974100263096061	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5835	0.6148039739411622	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2327	0.2451840355374609	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1979	0.20851706331269237	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1641	0.17290373971507234	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1458	0.1536219698382544	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1447	0.15246295634839105	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1446	0.15235759148567615	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1374	0.14477132137020682	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1321	0.13918698364631968	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1129	0.11895693000506805	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1099	0.11579598412362249	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.1078936194200086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0536486271485213E-4
7	0.0	0.0	0.0	0.0	1.0536486271485213E-4
8	0.0	0.0	0.0	0.0	1.0536486271485213E-4
9	0.0	0.0	0.0	1.0536486271485213E-4	1.0536486271485213E-4
10	0.0	0.0	0.0	1.0536486271485213E-4	1.0536486271485213E-4
11	0.0	0.0	0.0	1.0536486271485213E-4	1.0536486271485213E-4
12	0.0	0.0	0.0	1.0536486271485213E-4	5.268243135742606E-4
13	0.0	0.0	0.0	1.0536486271485213E-4	5.268243135742606E-4
14	0.0	0.0	0.0	2.1072972542970426E-4	5.268243135742606E-4
15	0.0	0.0	0.0	6.321891762891128E-4	5.268243135742606E-4
16	0.0	0.0	0.0	0.0010536486271485212	5.268243135742606E-4
17	0.0	0.0	0.0	0.0012643783525782255	5.268243135742606E-4
18	0.0	0.0	0.0	0.0018965675288673382	5.268243135742606E-4
19	0.0	0.0	0.0	0.0022126621170118948	5.268243135742606E-4
20	0.0	0.0	0.0	0.0028448512933010074	5.268243135742606E-4
21	0.0	0.0	0.0	0.0036877701950198244	5.268243135742606E-4
22	0.0	0.0	0.0	0.00495214854759805	5.268243135742606E-4
23	0.0	0.0	0.0	0.009798932232481248	5.268243135742606E-4
24	0.0	0.0	0.0	0.014434986191934741	6.321891762891128E-4
25	0.0	0.0	0.0	0.019176405014103087	6.321891762891128E-4
26	0.0	0.0	0.0	0.023917823836271432	6.321891762891128E-4
27	0.0	0.0	0.0	0.03245237771617446	6.321891762891128E-4
28	0.0	0.0	0.0	0.04614980986910523	6.321891762891128E-4
29	1.0536486271485213E-4	0.0	0.0	0.06332428249162612	6.321891762891128E-4
30	1.0536486271485213E-4	0.0	0.0	0.09366936295350355	6.321891762891128E-4
31	1.0536486271485213E-4	0.0	0.0	0.15067175368223854	7.375540390039648E-4
32	1.0536486271485213E-4	0.0	0.0	0.21958037389775184	7.375540390039648E-4
33	1.0536486271485213E-4	0.0	0.0	0.28164027803679975	7.375540390039648E-4
34	1.0536486271485213E-4	0.0	0.0	0.3652999790323923	7.375540390039648E-4
35	1.0536486271485213E-4	0.0	0.0	0.45612449069259486	7.375540390039648E-4
36	1.0536486271485213E-4	0.0	0.0	0.580033569245261	7.375540390039648E-4
37	1.0536486271485213E-4	0.0	0.0	0.7720083491117216	7.375540390039648E-4
38	1.0536486271485213E-4	0.0	0.0	0.978312750307402	8.42918901718817E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	50	8.3312225E-5	26.403889	1
TCGTATA	120	0.0	25.666391	44
GTACGAA	60	9.750911E-6	25.666391	19
ACGAATT	65	1.8130097E-5	23.692053	21
TGGTATA	445	0.0	22.74133	44
GTCGTAT	165	0.0	22.666422	43
CGGTGAT	185	0.0	20.216	43
CGATTAT	55	0.0044831936	19.999786	43
TCTATAC	90	1.00122525E-5	19.556376	3
TGTCGTA	70	8.122155E-4	18.85694	42
CTATACT	95	1.5959442E-5	18.527092	4
GTATTAA	120	3.15531E-7	18.336033	1
ATACCGT	240	0.0	18.334103	6
ACCGAAC	60	0.0074114185	18.333136	8
TACCGTC	240	0.0	18.333136	7
TACTCGT	110	2.7954284E-6	17.999807	36
CTATTCG	100	2.4823317E-5	17.59981	38
AAGTACT	515	0.0	17.515297	4
AGTACTC	515	0.0	17.515297	5
CGAATTT	90	2.2149221E-4	17.110928	22
>>END_MODULE