FastQCFastQC Report
Thu 2 Feb 2017
SRR4062376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751392
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG87580.5000593813378158No Hit
TCCATGTACTCTGCGTTGATACCAC76640.4375947817507445No Hit
GTATCAACGCAGAGTACTTTTTTTT48570.2773222670881219No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35900.2049798103451426No Hit
TATCAACGCAGAGTACTTTTTTTTT34670.19795682519961263No Hit
GAGTACATGGAAGCAGTGGTATCAA32690.18665153203851564No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG28990.16552547916171823No Hit
CATGTACTCTGCGTTGATACCACTG28230.16118607370594362No Hit
CTATTGGAGCTGGAATTACCGCGGC27220.15541923224498No Hit
GATTAAGAGGGACGGCCGGGGGCAT26980.15404889367999855No Hit
GTATCTGATCGTCTTCGAACCTCCG26820.15313533463667756No Hit
GAATAGGACCGCGGTTCTATTTTGT26480.1511940216696205No Hit
GTCTTGCGCCGGTCCAAGAATTTCA26420.15085143702837514No Hit
GAACTACGACGGTATCTGATCGTCT24690.14097357987246714No Hit
GTCCTATTCCATTATTCCTAGCTGC24570.14028841058997643No Hit
GAATAACGCCGCCGCATCGCCAGTC24360.13908936434561767No Hit
CCCATGTACTCTGCGTTGATACCAC24220.13829000018271181No Hit
ATCAGATACCGTCGTAGTTCCGACC24080.13749063601980596No Hit
GCGCAAGACGGACCAGAGCGAAAGC23680.13520673841150355No Hit
GTACATGGGAAGCAGTGGTATCAAC22740.12983957903199284No Hit
GCTTCCATGTACTCTGCGTTGATAC22500.1284692404670114No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21570.12315917852770825No Hit
GGGTAGGCACACGCTGAGCCAGTCA21340.12184593740293435No Hit
GTACATGGGGAATAATTGCAATCCC21250.12133206044106631No Hit
CTTTAATATACGCTATTGGAGCTGG21100.12047559883795289No Hit
TCGTAGTTCCGACCATAAACGATGC21030.12007591675649996No Hit
GCGTTGATACCACTGCTTCCATGTA20400.11647877802342366No Hit
GCTTTGAACACTCTAATTTTTTCAA19950.11390939321408343No Hit
GTCCTGTATTGTTATTTTTCGTCAC19540.11156839816557344No Hit
CTCTTAATCATGGCCTCAGTTCCGA19000.10848513639436516No Hit
ATATTAAAGTTGCTGCAGTTAAAAA18830.10751447991083664No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG18620.10631543366647787No Hit
CATGGAAGCAGTGGTATCAACGCAG18570.10602994646544006No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18570.10602994646544006No Hit
GTGCATGGCCGTTCTTAGTTGGTGG18420.10517348486232667No Hit
GGTCGGGAGTGGGTAATTTGCGCGC18180.10380314629734519No Hit
ATACAGGACTCTTTCGAGGCCCTGT18020.10288958725402422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG350.002168426316.2878714
CGAACGA1300.014.61857416
CGCCAGT4150.013.05104118
ACTCTAA3150.012.96997210
CGCATCG4300.012.59505313
TCGCCAG4400.012.52581917
ATCGCCA4400.012.5254616
CGTCTTA1750.012.48736915
GCATCGC4550.012.32031314
AGCCCGT550.003065667612.092516
AAAGCGT550.003065667612.0925110
GTTTTAA1501.4551915E-1112.021861
AGAATTT5350.011.90013417
CGGACAT2650.011.8304025
CGGTCCA5450.011.68044710
CATCGCC4800.011.6786315
GCGTTAT2700.011.6000411
CCGCATC4600.011.567080512
CGTCGTA4550.011.48503810
GTCCTAA2750.011.3891321
Produced by FastQC (version 0.11.5)