##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062376_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1751392
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13659249328534	32.0	32.0	32.0	32.0	32.0
2	30.827307079169028	32.0	32.0	32.0	32.0	32.0
3	30.822940837916356	32.0	32.0	32.0	32.0	32.0
4	30.86910126345216	32.0	32.0	32.0	32.0	32.0
5	30.688555160695035	32.0	32.0	32.0	32.0	32.0
6	34.33458186402587	36.0	36.0	36.0	32.0	36.0
7	34.18981415925161	36.0	36.0	36.0	32.0	36.0
8	34.18414095759259	36.0	36.0	36.0	32.0	36.0
9	34.32952474374669	36.0	36.0	36.0	32.0	36.0
10	34.0168911357366	36.0	36.0	36.0	32.0	36.0
11	34.33986052237306	36.0	36.0	36.0	32.0	36.0
12	34.10902527817873	36.0	36.0	36.0	32.0	36.0
13	34.24176483619886	36.0	36.0	36.0	32.0	36.0
14	34.146402975461804	36.0	36.0	36.0	32.0	36.0
15	34.09674533171329	36.0	36.0	36.0	32.0	36.0
16	34.08993075222452	36.0	36.0	36.0	32.0	36.0
17	34.00031003910033	36.0	36.0	36.0	32.0	36.0
18	34.02564588624363	36.0	36.0	36.0	32.0	36.0
19	34.00361598088835	36.0	36.0	36.0	32.0	36.0
20	33.9735102135901	36.0	36.0	36.0	32.0	36.0
21	33.92396790667081	36.0	36.0	36.0	32.0	36.0
22	33.883713640350074	36.0	36.0	36.0	32.0	36.0
23	33.85797811112532	36.0	36.0	36.0	32.0	36.0
24	33.8321969039484	36.0	36.0	36.0	32.0	36.0
25	33.36529457711352	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	55.0
5	162.0
6	307.0
7	96.0
8	249.0
9	313.0
10	201.0
11	64.0
12	134.0
13	107.0
14	401.0
15	477.0
16	723.0
17	1022.0
18	1434.0
19	2025.0
20	2775.0
21	4208.0
22	5925.0
23	8251.0
24	10986.0
25	14972.0
26	20191.0
27	26255.0
28	35940.0
29	47992.0
30	61657.0
31	85146.0
32	123866.0
33	179017.0
34	398548.0
35	717884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15126234204664	17.631332053089572	11.919172192785753	25.29823341207803
2	16.709615051900645	20.05170936095886	36.69206264735255	26.546612939787945
3	18.734204681067315	24.574918204087226	27.94148380355277	28.749393311292685
4	12.623855809594838	15.740873459351082	35.04447702025379	36.59079371080029
5	15.081870580111051	36.25375624517707	32.51353276393959	16.15084041077229
6	34.28280587186234	34.54985930425583	17.22800250887417	13.939332315007661
7	30.5755058616761	30.29547784173303	20.798008435694115	18.331007860896754
8	28.994480518367222	31.424869785267134	19.667196631390294	19.91345306497535
9	27.618220266331374	14.743898954106418	18.858890095444934	38.778990684117275
10	17.002971464112054	25.733405133235692	29.957937604970198	27.30568579768205
11	37.429555362710566	20.947997022717217	21.87879806499486	19.74364954957736
12	25.655301587955005	23.627848845253574	27.62963953981665	23.087210026974766
13	29.622644637453817	19.092092453178587	25.269586503496065	26.015676405871535
14	24.407016669277375	20.238325274438804	24.118885940249463	31.23577211603436
15	25.846671647396352	26.762695287379913	22.581511813418132	24.80912125180561
16	26.466200226360737	25.202695750591626	23.89477655451521	24.43632746853243
17	24.223194848434957	25.51182929952619	25.407611181237765	24.85736467080109
18	25.755204834500127	23.400844436694403	26.974063730100422	23.869886998705045
19	25.771040968197852	25.30421985702067	24.968440262906334	23.95629891187515
20	25.898800608271117	23.33857376430638	25.41664284652588	25.34598278089663
21	27.521894091939746	23.848180380560997	23.5662071165661	25.063718410933156
22	25.941908068285723	24.360457502697702	24.283772447248285	25.41386198176829
23	23.888824722084674	24.011129192172973	25.268652432309423	26.83139365343293
24	24.733058544442216	25.5465617368762	24.5963502134674	25.124029505214185
25	25.143875143932316	24.2403746871223	24.613884051441726	26.00186611750366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	370.0
1	370.0
2	880.5
3	1391.0
4	1391.0
5	1391.0
6	3342.5
7	5294.0
8	5294.0
9	5294.0
10	4943.5
11	4593.0
12	4593.0
13	4593.0
14	4389.0
15	4185.0
16	4185.0
17	4185.0
18	6823.0
19	9461.0
20	9461.0
21	9461.0
22	16805.5
23	24150.0
24	24150.0
25	24150.0
26	37650.5
27	51151.0
28	51151.0
29	51151.0
30	61869.0
31	72587.0
32	72587.0
33	72587.0
34	90180.5
35	107774.0
36	107774.0
37	107774.0
38	117979.0
39	128184.0
40	128184.0
41	128184.0
42	149201.0
43	170218.0
44	170218.0
45	170218.0
46	211112.0
47	252006.0
48	252006.0
49	252006.0
50	241796.5
51	231587.0
52	231587.0
53	231587.0
54	208420.5
55	185254.0
56	185254.0
57	185254.0
58	174359.0
59	163464.0
60	163464.0
61	163464.0
62	148550.0
63	133636.0
64	133636.0
65	133636.0
66	112474.0
67	91312.0
68	91312.0
69	91312.0
70	69815.5
71	48319.0
72	48319.0
73	48319.0
74	38908.0
75	29497.0
76	29497.0
77	29497.0
78	25495.5
79	21494.0
80	21494.0
81	21494.0
82	15114.5
83	8735.0
84	8735.0
85	8735.0
86	6460.5
87	4186.0
88	4186.0
89	4186.0
90	2854.5
91	1523.0
92	1523.0
93	1523.0
94	890.0
95	257.0
96	257.0
97	257.0
98	510.5
99	764.0
100	764.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009306882753832381
2	0.0037113336134914398
3	0.004681990097019971
4	0.01501662677458844
5	0.036599459173046356
6	0.04602053680729386
7	0.06908790265114835
8	0.09078492993002138
9	0.09660886883119256
10	0.11910526027297144
11	0.12253110668542508
12	0.13417898448776744
13	0.12213142460397217
14	0.1248150042937275
15	0.1132242239315927
16	0.11259615208930954
17	0.1242440298916519
18	0.13115282015676671
19	0.13292284080320113
20	0.12401564013082166
21	0.1307531380753138
22	0.15307823719647
23	0.1414874568343352
24	0.13777612322084376
25	0.13166669711863477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1751392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.783610545797195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8917212966411	28.657877413275166
2	14.683932170667807	11.389918131761654
3	4.987983820328909	5.8035606568912215
4	2.182146161423976	3.3852602751469507
5	1.1423704151088083	2.2152624639310345
6	0.6672720569295886	1.5527531750430108
7	0.42672563069043556	1.1584972469425274
8	0.2988218726666142	0.9271512905654212
9	0.2252076180955073	0.7860928096946506
>10	1.158795589337093	8.33502147399963
>50	0.13111449478474688	3.6220342342296283
>100	0.15771315661425794	13.260581464639005
>500	0.030453864201456557	8.013865366980957
>1k	0.015447612276101152	9.955109421914571
>5k	2.942402338304981E-4	0.9370145749844164
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8758	0.5000593813378158	No Hit
TCCATGTACTCTGCGTTGATACCAC	7664	0.4375947817507445	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4857	0.2773222670881219	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3590	0.2049798103451426	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3467	0.19795682519961263	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3269	0.18665153203851564	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2899	0.16552547916171823	No Hit
CATGTACTCTGCGTTGATACCACTG	2823	0.16118607370594362	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2722	0.15541923224498	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2698	0.15404889367999855	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2682	0.15313533463667756	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2648	0.1511940216696205	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2642	0.15085143702837514	No Hit
GAACTACGACGGTATCTGATCGTCT	2469	0.14097357987246714	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2457	0.14028841058997643	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2436	0.13908936434561767	No Hit
CCCATGTACTCTGCGTTGATACCAC	2422	0.13829000018271181	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2408	0.13749063601980596	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2368	0.13520673841150355	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2274	0.12983957903199284	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2250	0.1284692404670114	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2157	0.12315917852770825	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2134	0.12184593740293435	No Hit
GTACATGGGGAATAATTGCAATCCC	2125	0.12133206044106631	No Hit
CTTTAATATACGCTATTGGAGCTGG	2110	0.12047559883795289	No Hit
TCGTAGTTCCGACCATAAACGATGC	2103	0.12007591675649996	No Hit
GCGTTGATACCACTGCTTCCATGTA	2040	0.11647877802342366	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1995	0.11390939321408343	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1954	0.11156839816557344	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1900	0.10848513639436516	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1883	0.10751447991083664	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1862	0.10631543366647787	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1857	0.10602994646544006	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1857	0.10602994646544006	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1842	0.10517348486232667	No Hit
GGTCGGGAGTGGGTAATTTGCGCGC	1818	0.10380314629734519	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1802	0.10288958725402422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.7097440207560615E-5	0.0
10	0.0	0.0	0.0	1.1419488041512123E-4	0.0
11	0.0	0.0	0.0	1.7129232062268183E-4	0.0
12	0.0	0.0	0.0	1.7129232062268183E-4	3.996820814529243E-4
13	0.0	0.0	0.0	1.7129232062268183E-4	4.567795216604849E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAG	35	0.0021684263	16.28787	14
CGAACGA	130	0.0	14.618574	16
CGCCAGT	415	0.0	13.051041	18
ACTCTAA	315	0.0	12.969972	10
CGCATCG	430	0.0	12.595053	13
TCGCCAG	440	0.0	12.525819	17
ATCGCCA	440	0.0	12.52546	16
CGTCTTA	175	0.0	12.487369	15
GCATCGC	455	0.0	12.320313	14
AGCCCGT	55	0.0030656676	12.09251	6
AAAGCGT	55	0.0030656676	12.09251	10
GTTTTAA	150	1.4551915E-11	12.02186	1
AGAATTT	535	0.0	11.900134	17
CGGACAT	265	0.0	11.830402	5
CGGTCCA	545	0.0	11.680447	10
CATCGCC	480	0.0	11.67863	15
GCGTTAT	270	0.0	11.600041	1
CCGCATC	460	0.0	11.5670805	12
CGTCGTA	455	0.0	11.485038	10
GTCCTAA	275	0.0	11.389132	1
>>END_MODULE