FastQCFastQC Report
Thu 2 Feb 2017
SRR4062376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751392
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38810.22159516544554275No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33540.1915048144561583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA29390.16780937677002064No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27840.15895927353784875No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27560.15736054521203705No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA27470.15684666825016902No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26010.14851044197986518No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC24800.1416016517147503No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24290.13868968226416473No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22920.13086733295572892No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22680.12949699439074747No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT22650.12932570207012478No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21930.12521468637518043No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20930.11950494235442437No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT20890.11927655259359413No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG19640.11213937256764905No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19520.11145420328515833No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19360.11054064424183736No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA18190.10386024373755275No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC17820.10174763844987302No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT17700.10106246916738229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA502.3618813E-630.800627
TATGCGT654.9336586E-420.30752239
ACCGTAT550.00448462119.999838
TTAACGG2700.019.55539135
TAGGACG6300.018.5083084
TCGTATA600.007413757518.3331844
TAACGGC2800.018.07127636
GCCTTAA1102.793533E-618.0029321
GTCCTAA4900.017.962111
GATATAC2700.017.9288461
CATAATG2150.017.39520322
GTATAAT1154.268468E-617.2201961
TAGGACA2950.017.1528874
CGCACGA902.2162293E-417.11096628
CGAAAAA6900.016.89840924
GGACGTG6400.016.844096
CGTCGTA7600.016.78933110
CGCAATA8200.016.63400736
ACCGTCG7300.016.5752038
CCTATAT800.001988183516.5008032
Produced by FastQC (version 0.11.5)