##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1751392
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25457179203742	32.0	32.0	32.0	32.0	32.0
2	31.433237105114102	32.0	32.0	32.0	32.0	32.0
3	31.51558874312547	32.0	32.0	32.0	32.0	32.0
4	31.620623481208092	32.0	32.0	32.0	32.0	32.0
5	31.550278292923572	32.0	32.0	32.0	32.0	32.0
6	35.15652920648262	36.0	36.0	36.0	36.0	36.0
7	35.16374004220643	36.0	36.0	36.0	36.0	36.0
8	35.11477670333084	36.0	36.0	36.0	36.0	36.0
9	35.22538814839853	36.0	36.0	36.0	36.0	36.0
10	35.11108192797501	36.0	36.0	36.0	36.0	36.0
11	35.242077159196796	36.0	36.0	36.0	36.0	36.0
12	35.13725939138696	36.0	36.0	36.0	36.0	36.0
13	35.18282029380059	36.0	36.0	36.0	36.0	36.0
14	35.142665377025814	36.0	36.0	36.0	36.0	36.0
15	35.11116072244249	36.0	36.0	36.0	36.0	36.0
16	35.113043225046134	36.0	36.0	36.0	36.0	36.0
17	35.08296372257039	36.0	36.0	36.0	36.0	36.0
18	35.09494904624436	36.0	36.0	36.0	36.0	36.0
19	35.06570487931885	36.0	36.0	36.0	36.0	36.0
20	35.054240284299574	36.0	36.0	36.0	36.0	36.0
21	35.03573957172352	36.0	36.0	36.0	36.0	36.0
22	35.01793373499479	36.0	36.0	36.0	36.0	36.0
23	34.969267302808284	36.0	36.0	36.0	32.0	36.0
24	34.94012020153112	36.0	36.0	36.0	32.0	36.0
25	34.91737657817325	36.0	36.0	36.0	32.0	36.0
26	34.86769095667903	36.0	36.0	36.0	32.0	36.0
27	34.84337144397142	36.0	36.0	36.0	32.0	36.0
28	34.81065175586048	36.0	36.0	36.0	32.0	36.0
29	34.783036578904095	36.0	36.0	36.0	32.0	36.0
30	34.75881755769125	36.0	36.0	36.0	32.0	36.0
31	34.75958494728764	36.0	36.0	36.0	32.0	36.0
32	34.711792105936304	36.0	36.0	36.0	32.0	36.0
33	34.673442610220896	36.0	36.0	36.0	32.0	36.0
34	34.648347714275275	36.0	36.0	36.0	32.0	36.0
35	34.59809968299501	36.0	36.0	36.0	32.0	36.0
36	34.57016361842466	36.0	36.0	36.0	32.0	36.0
37	34.53890962160384	36.0	36.0	36.0	32.0	36.0
38	34.48065481628328	36.0	36.0	36.0	32.0	36.0
39	34.42415176042827	36.0	36.0	36.0	32.0	36.0
40	34.38901513767335	36.0	36.0	36.0	32.0	36.0
41	34.36189899234437	36.0	36.0	36.0	32.0	36.0
42	34.27419161444154	36.0	36.0	36.0	32.0	36.0
43	34.29404724927372	36.0	36.0	36.0	32.0	36.0
44	34.242508244870365	36.0	36.0	36.0	32.0	36.0
45	34.1483003233999	36.0	36.0	36.0	32.0	36.0
46	34.18239035007583	36.0	36.0	36.0	32.0	36.0
47	34.119055014525586	36.0	36.0	36.0	32.0	36.0
48	34.08869916043924	36.0	36.0	36.0	32.0	36.0
49	34.09602419104347	36.0	36.0	36.0	32.0	36.0
50	33.553165710474865	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	13.0
21	54.0
22	179.0
23	474.0
24	1421.0
25	3286.0
26	6813.0
27	12724.0
28	21295.0
29	31417.0
30	44082.0
31	61489.0
32	89981.0
33	138980.0
34	302880.0
35	1036300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48498450138311	18.258330078281148	12.415854045744332	25.840831374591406
2	16.08729018539286	20.538249125796263	36.397309896374594	26.977150792436284
3	18.153103179719317	24.787867402923748	28.320964998478342	28.73806441887859
4	12.28786099245912	16.166501178780017	35.269988871677135	36.27564895708373
5	14.733366373718734	36.43878697630228	32.54948064168387	16.278366008295116
6	34.43311118521124	34.77267121316628	16.989706426460447	13.804511175162043
7	30.496102811993435	30.425530335601813	20.807860723276526	18.27050612912822
8	29.032335422338345	31.39811075989841	19.610515521368146	19.959038296395097
9	27.75700699786227	14.348129944638321	18.879725384151577	39.01513767334783
10	17.060201257057244	25.72228261862564	30.010300378213444	27.207215746103667
11	37.52124024775721	20.751379474155414	21.96932497122289	19.758055306864485
12	25.475107799967112	23.626692368127753	27.742104565968102	23.156095265937036
13	29.744169209406003	18.909987027461586	25.353376057444592	25.992467705687815
14	24.454262666496135	20.126676380844494	24.105911183789807	31.313149768869565
15	25.957238585079757	26.777842995742812	22.460819736529572	24.80409868264786
16	26.598100253969413	25.055727101642578	23.83646836345033	24.509704280937676
17	24.324537282344558	25.45221172644388	25.321458588368568	24.901792402842997
18	25.941822276223714	23.14341963421096	27.0057759770514	23.908982112513932
19	25.90459474520838	25.09615208930953	24.956605945442252	24.042647220039832
20	25.962834134220092	23.161291132995927	25.5173028082805	25.35857192450348
21	27.566472839889645	23.750650910818365	23.723244139518737	24.959632109773256
22	26.016334435694578	24.13897060166999	24.38797253841516	25.45672242422028
23	23.907554676508745	23.68715855730756	25.440792238402366	26.96449452778133
24	24.899051725713033	25.284573641994484	24.71045888070746	25.105915751585023
25	25.376386325848237	23.82150883411595	24.81066488827173	25.991439951764082
26	24.39402486707716	25.026207725055272	25.79354022400468	24.786227183862895
27	26.135325501087138	24.447924850633097	24.561720048966766	24.855029599313003
28	24.577364747583637	23.895906798706402	25.686825108256745	25.83990334545322
29	24.372327839798285	24.445412563263965	25.925149823683103	25.257109773254644
30	24.770239900604775	24.32173950777439	26.281837532659736	24.6261830589611
31	25.198584897041897	24.336413549907732	24.355997971898923	26.109003581151452
32	24.630579561857083	24.53231486725987	24.524435420511228	26.31267015037182
33	24.126294969943906	24.135030878295662	25.39528557855694	26.343388573203484
34	25.00165582576602	24.34440719153679	25.892661380205002	24.761275602492187
35	26.352010286674826	24.05509446200508	25.39979627633334	24.193098974986754
36	24.630750854177705	25.00399682081453	24.880780544846615	25.48447178016115
37	26.303306170177777	24.659756353803147	24.569542398275203	24.467395077743877
38	24.814433319325428	24.526605123239115	24.931711461511757	25.727250095923697
39	25.450555900677863	24.234723008898065	24.618360709652663	25.69636038077141
40	26.05795846960589	24.521180866419396	25.027463868739837	24.393396795234874
41	24.227471633991705	24.948098426851328	25.92406497231916	24.900364966837806
42	26.714464837112423	25.39865432752919	24.634233798030365	23.25264703732802
43	25.222965504010524	23.694181542453087	24.9101286291133	26.172724324423086
44	24.727588112769727	24.509019111655185	25.108599331274778	25.654793444300307
45	24.962715371544462	24.86662037967514	24.96534185379401	25.20532239498639
46	24.55097068669496	24.37899290220505	25.500103061056112	25.56993335004388
47	25.294337304269977	23.92868072938554	25.775326140578464	25.00165582576602
48	25.895173667574134	25.804902614605986	23.79524401162047	24.50467970619941
49	24.40053397526082	25.101918930770495	25.252313588277207	25.245233505691473
50	24.336527744788146	26.29536962598893	23.90036039904259	25.467742230180335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	30.5
2	30.0
3	118.0
4	206.0
5	230.0
6	254.0
7	276.0
8	298.0
9	392.0
10	486.0
11	716.0
12	946.0
13	1794.5
14	2643.0
15	3739.0
16	4835.0
17	5683.0
18	6531.0
19	7068.0
20	7605.0
21	8210.5
22	8816.0
23	9094.0
24	9372.0
25	9526.5
26	9681.0
27	12244.0
28	14807.0
29	17478.5
30	20150.0
31	22123.5
32	24097.0
33	28881.0
34	33665.0
35	39106.0
36	44547.0
37	54601.0
38	64655.0
39	68763.5
40	72872.0
41	79598.5
42	86325.0
43	86742.0
44	87159.0
45	101014.0
46	114869.0
47	126699.5
48	138530.0
49	138703.0
50	138876.0
51	126228.5
52	113581.0
53	111528.0
54	109475.0
55	115523.5
56	121572.0
57	120903.5
58	120235.0
59	110308.0
60	100381.0
61	89104.5
62	77828.0
63	68440.5
64	59053.0
65	51330.5
66	43608.0
67	38420.0
68	33232.0
69	31848.5
70	30465.0
71	23426.0
72	16387.0
73	15064.5
74	13742.0
75	9472.5
76	5203.0
77	5052.0
78	4901.0
79	4281.0
80	3661.0
81	2899.5
82	2138.0
83	1957.0
84	1776.0
85	1479.0
86	1182.0
87	833.5
88	485.0
89	303.0
90	121.0
91	83.5
92	46.0
93	32.5
94	19.0
95	11.5
96	4.0
97	5.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015302113975626246
2	0.004796184977435091
3	6.280718422831667E-4
4	2.8548720103780306E-4
5	0.0
6	4.567795216604849E-4
7	5.7097440207560615E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.7129232062268186E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1751392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.85026199712313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82865054618998	32.06427280795411
2	14.265798333871476	12.225863924090307
3	4.778458420388358	6.142745857680008
4	2.0811758918535856	3.567157289120901
5	1.1201222741150758	2.3998766457322187
6	0.6556173823623719	1.6856025962457408
7	0.41887626763212754	1.2564270468689611
8	0.28564032849024296	0.9791810330200977
9	0.2037842730144273	0.7858988540605331
>10	1.0773940792583956	8.396388010703088
>50	0.11334806374139653	3.4070684856653974
>100	0.13567652605299282	12.594918655344111
>500	0.02461962565084533	7.125950627354224
>1k	0.010837987378904738	7.368648166160298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3881	0.22159516544554275	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3354	0.1915048144561583	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2939	0.16780937677002064	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2784	0.15895927353784875	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2756	0.15736054521203705	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2747	0.15684666825016902	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2601	0.14851044197986518	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2480	0.1416016517147503	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2429	0.13868968226416473	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2292	0.13086733295572892	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2268	0.12949699439074747	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2265	0.12932570207012478	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2193	0.12521468637518043	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2093	0.11950494235442437	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	2089	0.11927655259359413	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1964	0.11213937256764905	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1952	0.11145420328515833	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1936	0.11054064424183736	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1819	0.10386024373755275	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1782	0.10174763844987302	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1770	0.10106246916738229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2838976083024246E-4
2	0.0	0.0	0.0	0.0	2.2838976083024246E-4
3	0.0	0.0	0.0	0.0	2.2838976083024246E-4
4	0.0	0.0	0.0	0.0	2.2838976083024246E-4
5	0.0	0.0	0.0	0.0	2.8548720103780306E-4
6	0.0	0.0	0.0	0.0	3.996820814529243E-4
7	0.0	0.0	0.0	0.0	3.996820814529243E-4
8	0.0	0.0	0.0	0.0	3.996820814529243E-4
9	0.0	0.0	0.0	0.0	3.996820814529243E-4
10	0.0	0.0	0.0	0.0	4.567795216604849E-4
11	0.0	0.0	0.0	5.7097440207560615E-5	4.567795216604849E-4
12	0.0	0.0	0.0	5.7097440207560615E-5	8.564616031134092E-4
13	0.0	0.0	0.0	5.7097440207560615E-5	9.135590433209698E-4
14	0.0	0.0	0.0	5.7097440207560615E-5	9.135590433209698E-4
15	0.0	0.0	0.0	5.7097440207560615E-5	9.135590433209698E-4
16	0.0	0.0	0.0	2.2838976083024246E-4	0.001027753923736091
17	0.0	0.0	0.0	4.567795216604849E-4	0.001027753923736091
18	0.0	0.0	0.0	4.567795216604849E-4	0.0010848513639436516
19	0.0	0.0	0.0	4.567795216604849E-4	0.0010848513639436516
20	0.0	0.0	0.0	6.280718422831667E-4	0.0012561436845663335
21	0.0	0.0	0.0	9.135590433209698E-4	0.0012561436845663335
22	0.0	0.0	0.0	0.0016558257660192579	0.0012561436845663335
23	0.0	0.0	0.0	0.0033116515320385158	0.0012561436845663335
24	0.0	0.0	0.0	0.00559554914034094	0.001313241124773894
25	0.0	0.0	0.0	0.006965887705322395	0.001313241124773894
26	0.0	0.0	0.0	0.009934954596115547	0.001484533445396576
27	0.0	0.0	0.0	0.01296111892711626	0.001484533445396576
28	0.0	0.0	0.0	0.019584421991193292	0.001484533445396576
29	0.0	0.0	0.0	0.028320330342950065	0.001484533445396576
30	0.0	0.0	0.0	0.045963439367086294	0.001484533445396576
31	5.7097440207560615E-5	0.0	0.0	0.09049944272898357	0.001484533445396576
32	5.7097440207560615E-5	0.0	0.0	0.13971743618790083	0.0015987283258116973
33	5.7097440207560615E-5	0.0	0.0	0.1833969779466847	0.0016558257660192579
34	5.7097440207560615E-5	0.0	0.0	0.23929537190988653	0.001770020646434379
35	5.7097440207560615E-5	0.0	0.0	0.3037583819042225	0.001770020646434379
36	5.7097440207560615E-5	0.0	0.0	0.39928239937147136	0.001770020646434379
37	5.7097440207560615E-5	0.0	0.0	0.5342036505819371	0.001770020646434379
38	5.7097440207560615E-5	0.0	0.0	0.7011565657488443	0.001770020646434379
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	50	2.3618813E-6	30.80062	7
TATGCGT	65	4.9336586E-4	20.307522	39
ACCGTAT	55	0.004484621	19.99983	8
TTAACGG	270	0.0	19.555391	35
TAGGACG	630	0.0	18.508308	4
TCGTATA	60	0.0074137575	18.33318	44
TAACGGC	280	0.0	18.071276	36
GCCTTAA	110	2.793533E-6	18.002932	1
GTCCTAA	490	0.0	17.96211	1
GATATAC	270	0.0	17.928846	1
CATAATG	215	0.0	17.395203	22
GTATAAT	115	4.268468E-6	17.220196	1
TAGGACA	295	0.0	17.152887	4
CGCACGA	90	2.2162293E-4	17.110966	28
CGAAAAA	690	0.0	16.898409	24
GGACGTG	640	0.0	16.84409	6
CGTCGTA	760	0.0	16.789331	10
CGCAATA	820	0.0	16.634007	36
ACCGTCG	730	0.0	16.575203	8
CCTATAT	80	0.0019881835	16.500803	2
>>END_MODULE