FastQCFastQC Report
Thu 2 Feb 2017
SRR4062375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70015
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1820.2599442976505035No Hit
TATCAACGCAGAGTACTTTTTTTTT1050.14996786402913662No Hit
GGTATCAACGCAGAGTACTTTTTTT790.11283296436477898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1458.731149E-1111.7931041
TATCAAC2002.3102984E-88.5499992
AACGCAG1803.743844E-78.4444456
ATCAACG2053.1513264E-77.87804843
ACGCAGA1951.2607034E-67.7948727
TCAACGC1951.2607034E-67.7948724
CAACGCA1951.2607034E-67.7948725
AGTACAT1855.044547E-67.70270313
GAGTACA1855.044547E-67.70270312
CGCAGAG2052.675566E-67.41463388
AGAGTAC2458.88449E-87.367347211
GCAGAGT2251.3839854E-67.1777789
CAGAGTA2404.9833216E-77.124999510
ACATGGG2200.00266852425.613636516
TACATGG2554.3352653E-45.58823542
GTACATG2605.6344754E-45.480769614
Produced by FastQC (version 0.11.5)