FastQCFastQC Report
Thu 2 Feb 2017
SRR4062375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70015
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC207.8058604E-444.0000043
TCTTTCG254.4000066E-544.010
CTTTAGG254.4000066E-544.01
TTCGAGG254.4000066E-544.013
TTTAGGA301.2886502E-436.6666642
TTTCGAG301.2886502E-436.66666412
TCGAGGC301.2886502E-436.66666414
GACTCTT301.2886502E-436.6666647
ATACAGG301.2886502E-436.6666641
CCTGTAA353.18723E-431.42857221
TACAGGA353.18723E-431.4285722
CCCTGTA353.18723E-431.42857220
AGGACTC454.0156778E-529.3333345
GTAATTG454.0156778E-529.33333424
GGAATGA406.965719E-427.50000230
ACTCTTT406.965719E-427.5000028
TAATTGG406.965719E-427.50000225
CGAGGCC406.965719E-427.50000215
CTTTCGA406.965719E-427.50000211
AATATGG508.231763E-526.40000214
Produced by FastQC (version 0.11.5)