##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70015
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183889166607155	32.0	32.0	32.0	32.0	32.0
2	31.144240519888594	32.0	32.0	32.0	32.0	32.0
3	31.27055630936228	32.0	32.0	32.0	32.0	32.0
4	31.460415625223167	32.0	32.0	32.0	32.0	32.0
5	31.317232021709632	32.0	32.0	32.0	32.0	32.0
6	34.87011354709705	36.0	36.0	36.0	32.0	36.0
7	34.87072770120688	36.0	36.0	36.0	32.0	36.0
8	34.805398843105046	36.0	36.0	36.0	32.0	36.0
9	35.00852674426908	36.0	36.0	36.0	36.0	36.0
10	34.80402770834821	36.0	36.0	36.0	32.0	36.0
11	35.004284796115115	36.0	36.0	36.0	36.0	36.0
12	34.85173177176319	36.0	36.0	36.0	32.0	36.0
13	34.921659644361924	36.0	36.0	36.0	32.0	36.0
14	34.876255088195386	36.0	36.0	36.0	32.0	36.0
15	34.86678568878098	36.0	36.0	36.0	32.0	36.0
16	34.85423123616368	36.0	36.0	36.0	32.0	36.0
17	34.807169892165966	36.0	36.0	36.0	32.0	36.0
18	34.842233807041346	36.0	36.0	36.0	32.0	36.0
19	34.824623295008216	36.0	36.0	36.0	32.0	36.0
20	34.8164107691209	36.0	36.0	36.0	32.0	36.0
21	34.812183103620654	36.0	36.0	36.0	32.0	36.0
22	34.76987788331072	36.0	36.0	36.0	32.0	36.0
23	34.72812968649575	36.0	36.0	36.0	32.0	36.0
24	34.71676069413697	36.0	36.0	36.0	32.0	36.0
25	34.68562450903378	36.0	36.0	36.0	32.0	36.0
26	34.64129115189602	36.0	36.0	36.0	32.0	36.0
27	34.608169677926156	36.0	36.0	36.0	32.0	36.0
28	34.59470113547097	36.0	36.0	36.0	32.0	36.0
29	34.56520745554524	36.0	36.0	36.0	32.0	36.0
30	34.53151467542669	36.0	36.0	36.0	32.0	36.0
31	34.530143540669854	36.0	36.0	36.0	32.0	36.0
32	34.491523245018925	36.0	36.0	36.0	32.0	36.0
33	34.474512604441905	36.0	36.0	36.0	32.0	36.0
34	34.453531386131544	36.0	36.0	36.0	32.0	36.0
35	34.421438263229305	36.0	36.0	36.0	32.0	36.0
36	34.41249732200243	36.0	36.0	36.0	32.0	36.0
37	34.40766978504606	36.0	36.0	36.0	32.0	36.0
38	34.36342212383061	36.0	36.0	36.0	32.0	36.0
39	34.36709276583589	36.0	36.0	36.0	32.0	36.0
40	34.33929872170249	36.0	36.0	36.0	32.0	36.0
41	34.30492037420553	36.0	36.0	36.0	32.0	36.0
42	34.28421052631579	36.0	36.0	36.0	32.0	36.0
43	34.25628793829894	36.0	36.0	36.0	32.0	36.0
44	34.21553952724416	36.0	36.0	36.0	32.0	36.0
45	34.1716203670642	36.0	36.0	36.0	32.0	36.0
46	34.185146040134256	36.0	36.0	36.0	32.0	36.0
47	34.12513032921517	36.0	36.0	36.0	32.0	36.0
48	34.11738913090052	36.0	36.0	36.0	32.0	36.0
49	34.080239948582445	36.0	36.0	36.0	32.0	36.0
50	33.471784617581946	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	9.0
23	24.0
24	67.0
25	138.0
26	279.0
27	500.0
28	849.0
29	1393.0
30	1917.0
31	2801.0
32	4407.0
33	7200.0
34	14999.0
35	35431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57834595057849	18.908727324667904	12.705327810312813	26.807598914440796
2	17.104699328667333	20.615626339094415	35.592058277388944	26.687616054849308
3	19.838606012997214	23.086481468256803	27.67692637292009	29.397986145825893
4	14.465471684639006	16.52503035063915	33.095765193172895	35.91373277154896
5	16.70070699135899	33.928443904877525	31.43469256587874	17.93615653788474
6	34.37263443547811	33.49139470113547	16.480754124116263	15.655216739270156
7	30.299221595372423	29.41369706491466	20.01428265371706	20.27279868599586
8	28.006855673784187	31.15189602228094	19.450117831893166	21.391130472041706
9	27.167035635221026	15.09533671356138	19.378704563307863	38.35892308790974
10	17.791901735342428	25.461686781403987	29.750767692637293	26.9956437906163
11	36.402199528672426	21.426837106334357	21.961008355352423	20.209955009640794
12	25.68878097550525	23.95058201813897	26.81711061915304	23.543526387202743
13	29.6579304434764	19.690066414339785	24.66899950010712	25.983003642076696
14	24.489038063272154	20.517032064557593	24.109119474398344	30.884810397771904
15	25.843033635649505	27.21702492323074	21.93387131329001	25.006070127829748
16	26.382917946154393	25.671641791044774	23.19645790187817	24.748982360922657
17	24.656145111761766	25.535956580732698	24.48332500178533	25.324573305720204
18	25.190316360779835	24.48760979790045	25.904449046632866	24.417624794686855
19	26.1715346711419	24.573305720202814	25.1831750339213	24.071984574733985
20	26.34149825037492	23.81775333857031	24.834678283225024	25.006070127829748
21	26.977076340784116	23.90630579161608	24.333357137756195	24.783260729843605
22	26.43576376490752	24.140541312575877	24.446190102120973	24.97750482039563
23	24.93322859387274	24.48332500178533	25.05320288509605	25.530243519245875
24	25.251731771763193	25.01321145468828	24.686138684567595	25.048918088980933
25	25.313147182746555	24.579018781689637	24.790402056702135	25.31743197886167
26	24.724701849603655	25.156037991858888	25.537384846104406	24.58187531243305
27	25.146040134256946	24.684710419195888	25.003213597086337	25.166035849460826
28	24.6875669499393	24.644718988788117	25.1588945226023	25.508819538670284
29	24.69756480754124	24.569020924087695	25.316003713489966	25.417410554881098
30	24.728986645718777	24.98036135113904	25.368849532243093	24.92180247089909
31	25.600228522459474	24.343354995358137	24.564736127972576	25.491680354209812
32	25.03035063914875	24.526172962936513	24.54902520888381	25.89445118903092
33	24.39191601799614	24.401913875598087	25.521673927015637	25.68449617939013
34	25.341712490180672	24.24480468471042	25.35456687852603	25.05891594658288
35	25.60451331857459	24.17910447761194	25.11461829607941	25.10176390773406
36	24.42619438691709	25.026065843033635	24.968935228165392	25.578804541883883
37	25.374562593729916	24.526172962936513	24.751838891666072	25.347425551667502
38	24.68042562308077	24.100549882168107	25.60879811468971	25.610226380061412
39	25.358851674641148	23.883453545668782	24.95179604370492	25.805898735985146
40	25.20174248375348	23.930586302935087	25.701635363850606	25.166035849460826
41	24.397629079482968	24.409055202456617	25.880168535313864	25.313147182746555
42	25.365993001499675	24.493322859387273	25.351710347782618	24.78897379133043
43	24.649003784903233	23.789188031136185	25.491680354209812	26.070127829750767
44	24.126258658858816	24.087695493822753	25.690209240876953	26.095836606441473
45	24.286224380489895	24.607584089123762	25.74162679425837	25.36456473612797
46	24.289080911233306	23.81775333857031	25.893022923659213	26.00014282653717
47	24.75612368778119	23.74348353924159	25.87017067771192	25.6302220952653
48	24.39191601799614	24.430479183032208	25.693065771620365	25.484539027351282
49	23.88059701492537	24.61472541598229	25.540241376847817	25.964436192244516
50	23.73348568163965	24.650432050274944	25.788759551524677	25.827322716560737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	7.0
4	10.0
5	10.0
6	10.0
7	9.0
8	8.0
9	15.0
10	22.0
11	37.5
12	53.0
13	72.0
14	91.0
15	125.0
16	159.0
17	199.0
18	239.0
19	253.5
20	268.0
21	284.0
22	300.0
23	308.5
24	317.0
25	356.0
26	395.0
27	470.5
28	546.0
29	662.0
30	778.0
31	906.5
32	1035.0
33	1275.5
34	1516.0
35	1724.0
36	1932.0
37	2301.0
38	2670.0
39	2833.5
40	2997.0
41	3166.5
42	3336.0
43	3467.5
44	3599.0
45	3924.5
46	4250.0
47	4515.0
48	4780.0
49	4963.5
50	5147.0
51	5012.5
52	4878.0
53	4815.0
54	4752.0
55	4890.5
56	5029.0
57	4931.5
58	4834.0
59	4361.5
60	3889.0
61	3533.0
62	3177.0
63	2842.0
64	2507.0
65	2162.0
66	1817.0
67	1613.0
68	1409.0
69	1277.0
70	1145.0
71	928.0
72	711.0
73	596.5
74	482.0
75	381.0
76	280.0
77	258.0
78	236.0
79	198.5
80	161.0
81	136.0
82	111.0
83	86.5
84	62.0
85	54.5
86	47.0
87	31.0
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0071413268585303145
2	0.0071413268585303145
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	70015.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.97714775405271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89392099435673	71.89459401556809
2	6.773698121294378	10.834821109762194
3	1.3179512822344455	3.1621795329572233
4	0.5571826559039932	1.7824751838891668
5	0.30895063933138084	1.2354495465257445
6	0.20358597042645904	0.9769335142469471
7	0.16429744981784414	0.9198028993787046
8	0.11607971997999857	0.7426979932871528
9	0.11072219444246018	0.7969720774119832
>10	0.5464676048289163	7.33557094908234
>50	0.007143367383384528	0.318503177890452
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.001428265371706063	0.0
10	0.0	0.0	0.0	0.001428265371706063	0.0
11	0.0	0.0	0.0	0.001428265371706063	0.0
12	0.0	0.0	0.0	0.001428265371706063	0.0
13	0.0	0.0	0.0	0.001428265371706063	0.0
14	0.0	0.0	0.0	0.001428265371706063	0.0
15	0.0	0.0	0.0	0.001428265371706063	0.0
16	0.0	0.0	0.0	0.001428265371706063	0.001428265371706063
17	0.0	0.0	0.0	0.001428265371706063	0.001428265371706063
18	0.0	0.0	0.0	0.001428265371706063	0.001428265371706063
19	0.0	0.0	0.0	0.001428265371706063	0.001428265371706063
20	0.0	0.0	0.0	0.001428265371706063	0.001428265371706063
21	0.0	0.0	0.0	0.002856530743412126	0.001428265371706063
22	0.0	0.0	0.0	0.004284796115118189	0.001428265371706063
23	0.0	0.0	0.0	0.007141326858530315	0.001428265371706063
24	0.0	0.0	0.0	0.009997857601942441	0.001428265371706063
25	0.0	0.0	0.0	0.011426122973648504	0.001428265371706063
26	0.0	0.0	0.0	0.01428265371706063	0.001428265371706063
27	0.0	0.0	0.0	0.015710919088766692	0.001428265371706063
28	0.0	0.0	0.0	0.021423980575590945	0.001428265371706063
29	0.0	0.0	0.0	0.04284796115118189	0.001428265371706063
30	0.0	0.0	0.0	0.06998500321359709	0.001428265371706063
31	0.0	0.0	0.0	0.1342569449403699	0.001428265371706063
32	0.0	0.0	0.0	0.23852031707491253	0.001428265371706063
33	0.0	0.0	0.0	0.3756337927586946	0.001428265371706063
34	0.0	0.0	0.0	0.5613082910804827	0.001428265371706063
35	0.0	0.0	0.0	0.7569806470042134	0.001428265371706063
36	0.0	0.0	0.0	0.9883596372205956	0.001428265371706063
37	0.0	0.0	0.0	1.2768692423052204	0.001428265371706063
38	0.0	0.0	0.0	1.6210811968863814	0.001428265371706063
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGAC	20	7.8058604E-4	44.000004	3
TCTTTCG	25	4.4000066E-5	44.0	10
CTTTAGG	25	4.4000066E-5	44.0	1
TTCGAGG	25	4.4000066E-5	44.0	13
TTTAGGA	30	1.2886502E-4	36.666664	2
TTTCGAG	30	1.2886502E-4	36.666664	12
TCGAGGC	30	1.2886502E-4	36.666664	14
GACTCTT	30	1.2886502E-4	36.666664	7
ATACAGG	30	1.2886502E-4	36.666664	1
CCTGTAA	35	3.18723E-4	31.428572	21
TACAGGA	35	3.18723E-4	31.428572	2
CCCTGTA	35	3.18723E-4	31.428572	20
AGGACTC	45	4.0156778E-5	29.333334	5
GTAATTG	45	4.0156778E-5	29.333334	24
GGAATGA	40	6.965719E-4	27.500002	30
ACTCTTT	40	6.965719E-4	27.500002	8
TAATTGG	40	6.965719E-4	27.500002	25
CGAGGCC	40	6.965719E-4	27.500002	15
CTTTCGA	40	6.965719E-4	27.500002	11
AATATGG	50	8.231763E-5	26.400002	14
>>END_MODULE