FastQCFastQC Report
Thu 2 Feb 2017
SRR4062374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793359
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG63300.7978733461144324No Hit
TCCATGTACTCTGCGTTGATACCAC51510.649264708662787No Hit
GAGTACATGGAAGCAGTGGTATCAA24350.3069228432525502No Hit
GTATCAACGCAGAGTACTTTTTTTT23930.301628896880227No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19310.24339548678467124No Hit
CATGTACTCTGCGTTGATACCACTG18420.2321773623290339No Hit
GCTTCCATGTACTCTGCGTTGATAC16660.20999320610215552No Hit
TATCAACGCAGAGTACTTTTTTTTT16210.20432112070323774No Hit
CATGGAAGCAGTGGTATCAACGCAG15040.18957369866605156No Hit
GCGTTGATACCACTGCTTCCATGTA13570.17104488636292017No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13190.16625512535938963No Hit
CCCATGTACTCTGCGTTGATACCAC11920.15024723990022174No Hit
GTATCAACGCAGAGTACATGGAAGC10870.1370123739694136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10100.1273068056201543No Hit
GGTATCAACGCAGAGTACTTTTTTT8710.10978636405460832No Hit
ACGCAGAGTACATGGAAGCAGTGGT8250.10398823231349237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC704.4497028E-714.9221263
ATTAGAG1600.014.2438483
ACGGTAT1152.5465852E-1114.0445049
GACTCGC759.654395E-713.9334727
GGTCTAG551.9728078E-413.8061171
GCATCGC903.63234E-813.72149314
TTAGAGT852.7013266E-713.4085134
CGCGTAA500.001497590913.30097110
CGCCCCT655.4550816E-513.1514875
GGTCGGC604.08557E-412.66759211
AACTGTC905.3931035E-712.6667927
AGAGCGA1750.012.48820415
AAGACGG1750.012.4834755
AGGCCCG1457.2759576E-1212.44918510
AATTAGA1001.4530269E-712.3407752
CGACCAT853.9332062E-612.29501610
AGTGACG701.084371E-412.22057919
TTCGATT550.003064484612.09179310
TCGCGTA550.003064484612.0917939
GCGTAAC550.003064484612.09179311
Produced by FastQC (version 0.11.5)