##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062374_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793359
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.048813966943086	32.0	32.0	32.0	32.0	32.0
2	30.71479746243504	32.0	32.0	32.0	32.0	32.0
3	30.72038383632126	32.0	32.0	32.0	32.0	32.0
4	30.769588546925164	32.0	32.0	32.0	32.0	32.0
5	30.56993996412721	32.0	32.0	32.0	32.0	32.0
6	34.197185637271396	36.0	36.0	36.0	32.0	36.0
7	34.05425412707236	36.0	36.0	36.0	32.0	36.0
8	34.0330228811925	36.0	36.0	36.0	32.0	36.0
9	34.1888539740521	36.0	36.0	36.0	32.0	36.0
10	33.85075104712999	36.0	36.0	36.0	32.0	36.0
11	34.23175258615583	36.0	36.0	36.0	32.0	36.0
12	33.992873339812114	36.0	36.0	36.0	32.0	36.0
13	34.12296324866801	36.0	36.0	36.0	32.0	36.0
14	34.020331274996565	36.0	36.0	36.0	32.0	36.0
15	33.97085430429352	36.0	36.0	36.0	32.0	36.0
16	33.94760253554822	36.0	36.0	36.0	32.0	36.0
17	33.872665464184564	36.0	36.0	36.0	32.0	36.0
18	33.88216557699604	36.0	36.0	36.0	32.0	36.0
19	33.86937817558003	36.0	36.0	36.0	32.0	36.0
20	33.85050777768954	36.0	36.0	36.0	32.0	36.0
21	33.808683080421346	36.0	36.0	36.0	32.0	36.0
22	33.75910779357138	36.0	36.0	36.0	32.0	36.0
23	33.69232087869426	36.0	36.0	36.0	27.0	36.0
24	33.68001119291519	36.0	36.0	36.0	27.0	36.0
25	33.15226650230224	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	46.0
5	80.0
6	126.0
7	32.0
8	105.0
9	151.0
10	79.0
11	33.0
12	54.0
13	50.0
14	170.0
15	235.0
16	343.0
17	504.0
18	631.0
19	831.0
20	1356.0
21	2002.0
22	3019.0
23	4221.0
24	5748.0
25	7731.0
26	10467.0
27	13183.0
28	17797.0
29	23649.0
30	30403.0
31	41412.0
32	58918.0
33	83539.0
34	178175.0
35	308264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86920157267706	17.291758320023902	11.593514156740792	25.24552595055824
2	15.755632278663706	20.575574572466152	37.401884968279234	26.266908180590903
3	18.920107900973125	25.49450410931276	27.990722533151818	27.594665456562296
4	12.358488187388113	15.96782733667835	35.68556012203424	35.988124353899295
5	13.990278224357397	37.222295776512645	33.91890954713674	14.868516451993216
6	33.55688109260123	34.889718400443904	17.727404567638118	13.825995939316746
7	29.216572656730875	30.74906915027799	21.848859267630647	18.185498925360484
8	28.372529007237773	32.474985712429365	20.291251969666195	18.861233310666663
9	27.30610452792862	14.86742233078305	18.53349516523003	39.2929779760583
10	15.513353280491451	27.505498929238907	32.16359361808722	24.817554172182422
11	36.62839194426251	21.627931335173244	22.00399026285005	19.739686457714193
12	23.86424017841479	24.118938144238815	29.41660534815243	22.600216329193966
13	29.915753023603287	20.14633432269267	25.30589375492156	24.632018898782484
14	23.91869773006344	19.60673951698698	25.504889569801147	30.969673183148434
15	25.39564968315218	26.540264027235537	24.010528786337815	24.053557503274472
16	25.73357022261367	25.781898664217017	24.452803428164756	24.031727685004554
17	23.500564729340056	25.66306796312538	26.49684824239824	24.339519065136322
18	24.720235548605878	24.56904221928586	27.43919138071818	23.271530851390082
19	24.582345227622955	26.778116997605828	25.52486871182988	23.114669062941335
20	24.539197548780827	24.352302182423003	27.148539540800233	23.95996072799594
21	25.816352931525177	25.211179614683182	24.385140123811265	24.587327329980376
22	24.402069702887307	25.500415935045968	25.35221935114133	24.745295010925396
23	23.761583920470752	24.868793404202144	25.450040516899712	25.91958215842739
24	23.560081887966962	26.484093186688913	25.337813106618434	24.618011818725687
25	24.91900521875217	24.922160449809738	25.1086977099333	25.05013662150479
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	234.0
1	234.0
2	478.0
3	722.0
4	722.0
5	722.0
6	1868.5
7	3015.0
8	3015.0
9	3015.0
10	2985.0
11	2955.0
12	2955.0
13	2955.0
14	2930.5
15	2906.0
16	2906.0
17	2906.0
18	4667.5
19	6429.0
20	6429.0
21	6429.0
22	9834.5
23	13240.0
24	13240.0
25	13240.0
26	19249.0
27	25258.0
28	25258.0
29	25258.0
30	30653.5
31	36049.0
32	36049.0
33	36049.0
34	42490.0
35	48931.0
36	48931.0
37	48931.0
38	55422.0
39	61913.0
40	61913.0
41	61913.0
42	72682.5
43	83452.0
44	83452.0
45	83452.0
46	102891.0
47	122330.0
48	122330.0
49	122330.0
50	114235.0
51	106140.0
52	106140.0
53	106140.0
54	95979.5
55	85819.0
56	85819.0
57	85819.0
58	78214.5
59	70610.0
60	70610.0
61	70610.0
62	62522.5
63	54435.0
64	54435.0
65	54435.0
66	44143.5
67	33852.0
68	33852.0
69	33852.0
70	25385.0
71	16918.0
72	16918.0
73	16918.0
74	12947.5
75	8977.0
76	8977.0
77	8977.0
78	7172.0
79	5367.0
80	5367.0
81	5367.0
82	3715.5
83	2064.0
84	2064.0
85	2064.0
86	1520.0
87	976.0
88	976.0
89	976.0
90	628.5
91	281.0
92	281.0
93	281.0
94	175.5
95	70.0
96	70.0
97	70.0
98	243.0
99	416.0
100	416.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007814873216286699
2	0.0037813902659451773
3	0.004915807345728731
4	0.017520441565545986
5	0.03705762460626274
6	0.04903202711508914
7	0.06743479307602233
8	0.08974499564509887
9	0.09667754446599837
10	0.11747519092869683
11	0.1172230982443005
12	0.13209656662368485
13	0.11684495921770598
14	0.12075239582584932
15	0.1090300860014193
16	0.1090300860014193
17	0.11898774703507492
18	0.1255421568293799
19	0.12831517635773967
20	0.11810542263968771
21	0.1290714544109287
22	0.14722212768746556
23	0.13827283739139531
24	0.13323098370346842
25	0.1290714544109287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	793359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.223517379095455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53942309375232	45.28209654013905
2	12.217893280307482	13.738658703626024
3	3.330874714393217	5.6182047717683
4	1.3423087049577673	3.0187726720521657
5	0.6481955211474754	1.8221916074143456
6	0.38094057894757555	1.2850691556517018
7	0.2647690368513318	1.0420372581400053
8	0.18280229493764255	0.8222230405092062
9	0.13249608342440317	0.6704456264166612
>10	0.7743104892205664	8.403223663074957
>50	0.09456579274265797	3.8034785059240237
>100	0.0837833235283169	8.972732773002825
>500	0.004492403405425018	1.626354384125069
>1k	0.002695442043255011	2.44458315060638
>5k	4.492403405425018E-4	1.4499281475493024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6330	0.7978733461144324	No Hit
TCCATGTACTCTGCGTTGATACCAC	5151	0.649264708662787	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2435	0.3069228432525502	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2393	0.301628896880227	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1931	0.24339548678467124	No Hit
CATGTACTCTGCGTTGATACCACTG	1842	0.2321773623290339	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1666	0.20999320610215552	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1621	0.20432112070323774	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1504	0.18957369866605156	No Hit
GCGTTGATACCACTGCTTCCATGTA	1357	0.17104488636292017	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1319	0.16625512535938963	No Hit
CCCATGTACTCTGCGTTGATACCAC	1192	0.15024723990022174	No Hit
GTATCAACGCAGAGTACATGGAAGC	1087	0.1370123739694136	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1010	0.1273068056201543	No Hit
GGTATCAACGCAGAGTACTTTTTTT	871	0.10978636405460832	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	825	0.10398823231349237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2604634219817257E-4
2	0.0	0.0	0.0	0.0	1.2604634219817257E-4
3	0.0	0.0	0.0	0.0	1.2604634219817257E-4
4	0.0	0.0	0.0	0.0	1.2604634219817257E-4
5	0.0	0.0	0.0	0.0	1.2604634219817257E-4
6	0.0	0.0	0.0	0.0	1.2604634219817257E-4
7	0.0	0.0	0.0	0.0	1.2604634219817257E-4
8	0.0	0.0	0.0	0.0	1.2604634219817257E-4
9	0.0	0.0	0.0	2.5209268439634514E-4	1.2604634219817257E-4
10	1.2604634219817257E-4	0.0	0.0	2.5209268439634514E-4	2.5209268439634514E-4
11	1.2604634219817257E-4	0.0	0.0	2.5209268439634514E-4	2.5209268439634514E-4
12	1.2604634219817257E-4	0.0	0.0	2.5209268439634514E-4	5.041853687926903E-4
13	1.2604634219817257E-4	0.0	0.0	3.7813902659451776E-4	5.041853687926903E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	70	4.4497028E-7	14.922126	3
ATTAGAG	160	0.0	14.243848	3
ACGGTAT	115	2.5465852E-11	14.044504	9
GACTCGC	75	9.654395E-7	13.933472	7
GGTCTAG	55	1.9728078E-4	13.806117	1
GCATCGC	90	3.63234E-8	13.721493	14
TTAGAGT	85	2.7013266E-7	13.408513	4
CGCGTAA	50	0.0014975909	13.300971	10
CGCCCCT	65	5.4550816E-5	13.151487	5
GGTCGGC	60	4.08557E-4	12.667592	11
AACTGTC	90	5.3931035E-7	12.666792	7
AGAGCGA	175	0.0	12.488204	15
AAGACGG	175	0.0	12.483475	5
AGGCCCG	145	7.2759576E-12	12.449185	10
AATTAGA	100	1.4530269E-7	12.340775	2
CGACCAT	85	3.9332062E-6	12.295016	10
AGTGACG	70	1.084371E-4	12.220579	19
TTCGATT	55	0.0030644846	12.091793	10
TCGCGTA	55	0.0030644846	12.091793	9
GCGTAAC	55	0.0030644846	12.091793	11
>>END_MODULE