FastQCFastQC Report
Thu 2 Feb 2017
SRR4062374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793359
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25660.32343491408051084No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22300.28108334310192484No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8630.10877799331702294No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7970.10045893473194356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAC300.00574395829.3332083
GATATAT1254.0017767E-1122.8827881
TATGGCG500.00257972821.99990716
ATACCGT1205.984475E-1021.9999076
ACCGTCG1259.949872E-1021.119918
TACCGTC1259.949872E-1021.119917
TTAGGAC856.109296E-620.7057933
GTCGTAT550.00448238819.99991443
CGTCGTA1352.593879E-919.55547110
CAATAGG808.975213E-519.2523441
TAACGGC1152.0192965E-719.13035236
TCGTTAA1504.638423E-1019.06658612
CAACGGA1051.7911789E-618.85706114
GTATTAG2000.018.7022761
GTATAAT951.5943093E-518.5285721
CGCAATC951.5960417E-518.52623644
CCGTCGT1557.203198E-1018.4515349
GTGCACG851.4300946E-418.1175699
AACGGAC1102.7940823E-617.99992415
GTATTAT1002.4786747E-517.6021421
Produced by FastQC (version 0.11.5)