##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793359
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161871233577738	32.0	32.0	32.0	32.0	32.0
2	31.330168309680737	32.0	32.0	32.0	32.0	32.0
3	31.455473499386784	32.0	32.0	32.0	32.0	32.0
4	31.586976387738716	32.0	32.0	32.0	32.0	32.0
5	31.508483549061648	32.0	32.0	32.0	32.0	32.0
6	35.09963963350766	36.0	36.0	36.0	36.0	36.0
7	35.122169156712154	36.0	36.0	36.0	36.0	36.0
8	35.05973966388483	36.0	36.0	36.0	36.0	36.0
9	35.19679489361059	36.0	36.0	36.0	36.0	36.0
10	35.05912707866174	36.0	36.0	36.0	36.0	36.0
11	35.19546132331013	36.0	36.0	36.0	36.0	36.0
12	35.106004973788664	36.0	36.0	36.0	36.0	36.0
13	35.15236733937599	36.0	36.0	36.0	36.0	36.0
14	35.104072683362766	36.0	36.0	36.0	36.0	36.0
15	35.064163890495976	36.0	36.0	36.0	36.0	36.0
16	35.082117931478685	36.0	36.0	36.0	36.0	36.0
17	35.049988718852376	36.0	36.0	36.0	36.0	36.0
18	35.06058417437755	36.0	36.0	36.0	36.0	36.0
19	35.02695501027908	36.0	36.0	36.0	36.0	36.0
20	35.01768808320067	36.0	36.0	36.0	36.0	36.0
21	34.998484922966774	36.0	36.0	36.0	36.0	36.0
22	34.970734560268426	36.0	36.0	36.0	36.0	36.0
23	34.90311699999622	36.0	36.0	36.0	32.0	36.0
24	34.88797883429822	36.0	36.0	36.0	32.0	36.0
25	34.8618645531216	36.0	36.0	36.0	32.0	36.0
26	34.8073041334377	36.0	36.0	36.0	32.0	36.0
27	34.77358925782653	36.0	36.0	36.0	32.0	36.0
28	34.74228816966846	36.0	36.0	36.0	32.0	36.0
29	34.713732370843466	36.0	36.0	36.0	32.0	36.0
30	34.6937968813614	36.0	36.0	36.0	32.0	36.0
31	34.67733522907032	36.0	36.0	36.0	32.0	36.0
32	34.62603310733224	36.0	36.0	36.0	32.0	36.0
33	34.586110449367816	36.0	36.0	36.0	32.0	36.0
34	34.55847730976771	36.0	36.0	36.0	32.0	36.0
35	34.48656787154365	36.0	36.0	36.0	32.0	36.0
36	34.46117457544441	36.0	36.0	36.0	32.0	36.0
37	34.409614058704825	36.0	36.0	36.0	32.0	36.0
38	34.357567003084355	36.0	36.0	36.0	32.0	36.0
39	34.25607700927323	36.0	36.0	36.0	32.0	36.0
40	34.196929763196735	36.0	36.0	36.0	32.0	36.0
41	34.19155892855567	36.0	36.0	36.0	32.0	36.0
42	34.07786512789292	36.0	36.0	36.0	32.0	36.0
43	34.10110555246742	36.0	36.0	36.0	32.0	36.0
44	34.06568653030973	36.0	36.0	36.0	32.0	36.0
45	33.92243864379178	36.0	36.0	36.0	32.0	36.0
46	34.0118181050445	36.0	36.0	36.0	32.0	36.0
47	33.918475746793064	36.0	36.0	36.0	32.0	36.0
48	33.91388513901021	36.0	36.0	36.0	32.0	36.0
49	33.93463488786287	36.0	36.0	36.0	32.0	36.0
50	33.36370672041283	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	6.0
21	21.0
22	74.0
23	265.0
24	680.0
25	1668.0
26	3489.0
27	6625.0
28	10989.0
29	15901.0
30	21870.0
31	30424.0
32	44453.0
33	66116.0
34	139780.0
35	450993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36048424611684	17.86309949283502	12.1556970020158	25.620719259032338
2	15.337277927499803	21.031813059514295	36.933300727825525	26.697608285160374
3	18.37883010463186	25.738227497721695	28.548704044731664	27.33423835291478
4	12.185591569978131	16.4307277322258	35.94355616338209	35.440124534413975
5	13.79715866335417	37.3775302227617	33.80096526288855	15.024345850995577
6	33.75596831687162	35.14928985222271	17.39219215585437	13.702549675051301
7	29.100949255003094	30.83559901633435	21.903703115487442	18.15974861317512
8	28.413114365627667	32.55726600441918	20.125441319755623	18.90417831019753
9	27.264706141860117	14.565159026367635	18.384237148630064	39.78589768314219
10	15.524749829522321	27.419112911052878	32.31008912736857	24.74604813205623
11	37.03770928419542	21.40014797840574	21.844839473680892	19.717303263717938
12	23.853009797582178	23.996828674030294	29.556732828391684	22.59342869999584
13	30.105790695006927	20.002924275138998	25.31590364513417	24.575381384719904
14	23.967207783613723	19.484999855046706	25.404892362726077	31.142899998613487
15	25.507368038933194	26.439228646804285	24.0171221351242	24.03628117913832
16	25.88273404599935	25.545686126961435	24.441646215647644	24.129933611391564
17	23.677931428268916	25.576315388115596	26.537040608350065	24.208712575265423
18	24.92692463311061	24.32631381253632	27.300377256702202	23.446384297650873
19	24.639412926556577	26.681237623824778	25.501821999876473	23.177527449742172
20	24.77327414197104	24.112035030799422	27.18025509258734	23.934435734642197
21	25.985461814890865	24.993477101791246	24.34610308826143	24.674957995056463
22	24.53706329669166	25.228931669017427	25.431866279956488	24.80213875433442
23	23.908092049122782	24.551432579702254	25.467537394798573	26.07293797637639
24	23.69368722104369	26.328559958354287	25.262207903357748	24.715544917244276
25	25.163891756443174	24.4861405744436	25.19452101759733	25.155446651515895
26	23.75091226040166	24.989443618840905	26.188396425829918	25.071247694927518
27	25.319685035400113	25.508880595039575	25.428084889690545	23.743349479869767
28	24.385681639711656	24.18879725319811	26.056425905548437	25.369095201541796
29	23.71208998700462	25.566483773424135	26.683254365299945	24.038171874271296
30	25.123178787913165	24.759661137013634	26.408347292965733	23.70881278210747
31	23.71410672847979	25.125069483046136	25.768914198994402	25.391909589479667
32	23.81154055099898	25.555013556284102	25.73110029633495	24.902345596381966
33	23.812044736367774	24.913311628153206	25.792610911327657	25.48203272415136
34	23.883008827025346	25.619927422516163	26.656028355385143	23.841035395073355
35	25.505855482826817	24.90272373540856	25.862314538563247	23.729106243201375
36	24.30589430510021	25.611356271246688	25.668077125235868	24.414672298417237
37	25.37035566496378	25.362540791747495	25.663161317890136	23.60394222539859
38	24.217661865561492	25.38157378941942	25.389892848004497	25.01087149701459
39	24.02947467665962	25.4999313047435	25.811391816315187	24.65920220228169
40	25.093053712127798	25.331281298882345	25.44812625810005	24.1275387308898
41	23.322228650585675	25.524636387814343	26.420069602790164	24.73306535880982
42	25.51556105117608	25.621566024964736	25.50358664866725	23.359286275191938
43	24.592019501890064	24.4239997277399	25.48203272415136	25.501948046218676
44	24.34736355168341	25.086121163306903	26.357550617059864	24.208964667949818
45	24.594288336049633	25.362918930774086	25.72139472798569	24.321398005190588
46	24.071196015927214	25.3137608573168	26.041678483511248	24.573364643244737
47	24.51702192828215	24.74957742963778	26.67859065063861	24.054809991441452
48	24.43509180585334	26.9349689106697	24.645967336350882	23.983971947126083
49	23.437687100039202	25.49438526568678	26.67468321403047	24.393244420243544
50	23.564363673948364	26.87106341517522	24.571347901769563	24.993225009106848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	36.5
2	41.0
3	143.0
4	245.0
5	262.0
6	279.0
7	247.0
8	215.0
9	280.0
10	345.0
11	495.5
12	646.0
13	1100.5
14	1555.0
15	2089.0
16	2623.0
17	3032.0
18	3441.0
19	3690.5
20	3940.0
21	4544.5
22	5149.0
23	5465.0
24	5781.0
25	6435.0
26	7089.0
27	8609.5
28	10130.0
29	11089.0
30	12048.0
31	12618.5
32	13189.0
33	14908.0
34	16627.0
35	18741.0
36	20855.0
37	25020.0
38	29185.0
39	31373.0
40	33561.0
41	37499.5
42	41438.0
43	42942.5
44	44447.0
45	50824.5
46	57202.0
47	61921.5
48	66641.0
49	64661.0
50	62681.0
51	58547.0
52	54413.0
53	53003.0
54	51593.0
55	51994.5
56	52396.0
57	50747.5
58	49099.0
59	45254.5
60	41410.0
61	36643.5
62	31877.0
63	27270.0
64	22663.0
65	19337.5
66	16012.0
67	13635.5
68	11259.0
69	10024.0
70	8789.0
71	6819.5
72	4850.0
73	4384.0
74	3918.0
75	2839.0
76	1760.0
77	1577.5
78	1395.0
79	1217.5
80	1040.0
81	836.5
82	633.0
83	524.5
84	416.0
85	336.0
86	256.0
87	188.0
88	120.0
89	80.5
90	41.0
91	29.0
92	17.0
93	11.0
94	5.0
95	5.5
96	6.0
97	5.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015881839116969745
2	0.004663714661332385
3	7.562780531890355E-4
4	5.041853687926903E-4
5	0.0
6	3.7813902659451776E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	793359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.35986732707488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92639018909136	48.847061750726496
2	12.060364036822852	14.559239463577054
3	3.2729218129855546	5.926593792110923
4	1.266386287097935	3.057556330962333
5	0.6663511001406163	2.0110431998868994
6	0.3938298835938675	1.426291171389791
7	0.2528298774739475	1.0682544502453646
8	0.16778110908249305	0.8101796387367008
9	0.11948323194697642	0.649079282531665
>10	0.7146988712196737	8.215667263441386
>50	0.08388647884629748	3.6223258057618364
>100	0.07088286909350383	7.808480209272083
>500	0.003774827344420795	1.3911390965741504
>1k	4.194252604911994E-4	0.6070885447834053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2566	0.32343491408051084	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2230	0.28108334310192484	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	863	0.10877799331702294	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	797	0.10045893473194356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.2604634219817257E-4
6	0.0	0.0	0.0	0.0	2.5209268439634514E-4
7	0.0	0.0	0.0	0.0	2.5209268439634514E-4
8	0.0	0.0	0.0	0.0	2.5209268439634514E-4
9	0.0	0.0	0.0	1.2604634219817257E-4	2.5209268439634514E-4
10	0.0	0.0	0.0	1.2604634219817257E-4	3.7813902659451776E-4
11	0.0	0.0	0.0	1.2604634219817257E-4	3.7813902659451776E-4
12	0.0	0.0	0.0	2.5209268439634514E-4	5.041853687926903E-4
13	0.0	0.0	0.0	3.7813902659451776E-4	6.30231710990863E-4
14	0.0	0.0	0.0	3.7813902659451776E-4	6.30231710990863E-4
15	0.0	0.0	0.0	3.7813902659451776E-4	0.0010083707375853805
16	0.0	0.0	0.0	3.7813902659451776E-4	0.0010083707375853805
17	0.0	0.0	0.0	3.7813902659451776E-4	0.0010083707375853805
18	0.0	0.0	0.0	6.30231710990863E-4	0.0010083707375853805
19	0.0	0.0	0.0	7.562780531890355E-4	0.0010083707375853805
20	0.0	0.0	0.0	7.562780531890355E-4	0.0010083707375853805
21	0.0	0.0	0.0	0.0017646487907744162	0.0010083707375853805
22	0.0	0.0	0.0	0.003277204897152487	0.0010083707375853805
23	0.0	0.0	0.0	0.006932548820899492	0.0010083707375853805
24	0.0	0.0	0.0	0.012100448851024568	0.0010083707375853805
25	0.0	0.0	0.0	0.015125561063780709	0.0010083707375853805
26	0.0	0.0	0.0	0.01953718304071675	0.0011344170797835532
27	0.0	0.0	0.0	0.024200897702049137	0.001260463421981726
28	0.0	0.0	0.0	0.03214181726053401	0.001260463421981726
29	0.0	0.0	0.0	0.0418473856097933	0.001260463421981726
30	0.0	0.0	0.0	0.06403154183667167	0.001260463421981726
31	0.0	0.0	0.0	0.10146730546952892	0.001260463421981726
32	0.0	0.0	0.0	0.14873468379384364	0.001260463421981726
33	0.0	0.0	0.0	0.19385927430078942	0.001260463421981726
34	0.0	0.0	0.0	0.24793315510380545	0.001512556106378071
35	0.0	0.0	0.0	0.31209074328267533	0.001512556106378071
36	0.0	0.0	0.0	0.40297015600755776	0.001512556106378071
37	0.0	0.0	0.0	0.5307811469965047	0.001512556106378071
38	0.0	0.0	0.0	0.6787595527371594	0.001512556106378071
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGAC	30	0.005743958	29.333208	3
GATATAT	125	4.0017767E-11	22.882788	1
TATGGCG	50	0.002579728	21.999907	16
ATACCGT	120	5.984475E-10	21.999907	6
ACCGTCG	125	9.949872E-10	21.11991	8
TACCGTC	125	9.949872E-10	21.11991	7
TTAGGAC	85	6.109296E-6	20.705793	3
GTCGTAT	55	0.004482388	19.999914	43
CGTCGTA	135	2.593879E-9	19.555471	10
CAATAGG	80	8.975213E-5	19.252344	1
TAACGGC	115	2.0192965E-7	19.130352	36
TCGTTAA	150	4.638423E-10	19.066586	12
CAACGGA	105	1.7911789E-6	18.857061	14
GTATTAG	200	0.0	18.702276	1
GTATAAT	95	1.5943093E-5	18.528572	1
CGCAATC	95	1.5960417E-5	18.526236	44
CCGTCGT	155	7.203198E-10	18.451534	9
GTGCACG	85	1.4300946E-4	18.117569	9
AACGGAC	110	2.7940823E-6	17.999924	15
GTATTAT	100	2.4786747E-5	17.602142	1
>>END_MODULE