FastQCFastQC Report
Thu 2 Feb 2017
SRR4062373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994270
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG137660.6902776454542263No Hit
TCCATGTACTCTGCGTTGATACCAC112910.566172083017846No Hit
GAGTACATGGAAGCAGTGGTATCAA49900.2502168713363787No Hit
CATGTACTCTGCGTTGATACCACTG42350.21235840683558396No Hit
GCTTCCATGTACTCTGCGTTGATAC34080.17088959870027629No Hit
CCCATGTACTCTGCGTTGATACCAC33750.16923485786779122No Hit
GTATCAACGCAGAGTACTTTTTTTT33370.16732939872735386No Hit
GTACATGGGAAGCAGTGGTATCAAC33210.16652710014190658No Hit
GCGTTGATACCACTGCTTCCATGTA30560.15323902982043555No Hit
CATGGAAGCAGTGGTATCAACGCAG28330.1420569932857637No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25290.1268133201622649No Hit
TATCAACGCAGAGTACTTTTTTTTT24220.12144794837208603No Hit
GTATCAACGCAGAGTACATGGAAGC23370.11718573713689721No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21220.10640484989494904No Hit
GAATAGGACCGCGGTTCTATTTTGT20750.10404809780019757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG551.9618406E-413.81714254
AATCGTA1051.369699E-913.57245113
GTCCTAA2100.012.6580851
CGCCAGT3250.012.57112518
CGACCAT3250.012.57080910
ACGGTAT3200.012.4703169
ACAGTCG853.9393562E-612.2956618
TCGGCAA701.09043096E-412.21520613
TCGCGTA1501.4551915E-1112.0348449
CGAACGA1353.7107384E-1011.96386316
TCGTTCA1459.640644E-1111.79399216
CCGACCA3500.011.6728949
CGTCGTA3550.011.50848710
CGAGCCG4400.011.44375715
ATCGCCA3250.011.40085916
TTTTCGG2850.011.334187516
TTTGCGC2450.011.2463117
AACGGAC1104.9670416E-711.22783715
GTAATAC1104.9896516E-711.2238913
GACGGTA3600.011.0847258
Produced by FastQC (version 0.11.5)