##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062373_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994270
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1042657212915	32.0	32.0	32.0	32.0	32.0
2	30.697867891509173	32.0	32.0	32.0	32.0	32.0
3	30.694289138381464	32.0	32.0	32.0	32.0	32.0
4	30.764848791788474	32.0	32.0	32.0	32.0	32.0
5	30.537326941687937	32.0	32.0	32.0	32.0	32.0
6	34.20512518365116	36.0	36.0	36.0	32.0	36.0
7	34.07580317609953	36.0	36.0	36.0	32.0	36.0
8	34.054692694569944	36.0	36.0	36.0	32.0	36.0
9	34.23952925130499	36.0	36.0	36.0	32.0	36.0
10	33.85644872559884	36.0	36.0	36.0	32.0	36.0
11	34.233089802283544	36.0	36.0	36.0	32.0	36.0
12	33.9711267782196	36.0	36.0	36.0	32.0	36.0
13	34.11117752360513	36.0	36.0	36.0	32.0	36.0
14	33.99056246145206	36.0	36.0	36.0	32.0	36.0
15	33.92307711593716	36.0	36.0	36.0	32.0	36.0
16	33.919412115711516	36.0	36.0	36.0	32.0	36.0
17	33.8361465598941	36.0	36.0	36.0	32.0	36.0
18	33.84212920015845	36.0	36.0	36.0	32.0	36.0
19	33.840644947775374	36.0	36.0	36.0	32.0	36.0
20	33.82724605996179	36.0	36.0	36.0	32.0	36.0
21	33.78602345720489	36.0	36.0	36.0	32.0	36.0
22	33.725814458423386	36.0	36.0	36.0	27.0	36.0
23	33.66325472478651	36.0	36.0	36.0	27.0	36.0
24	33.63098025844043	36.0	36.0	36.0	27.0	36.0
25	33.12616095112497	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	13.0
4	73.0
5	200.0
6	339.0
7	101.0
8	294.0
9	351.0
10	237.0
11	81.0
12	146.0
13	106.0
14	432.0
15	503.0
16	748.0
17	997.0
18	1402.0
19	2100.0
20	3207.0
21	4765.0
22	7211.0
23	10612.0
24	14835.0
25	20522.0
26	27706.0
27	35303.0
28	46739.0
29	60977.0
30	77329.0
31	104330.0
32	147100.0
33	204568.0
34	435291.0
35	785650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.5032345418986	17.04423047991575	11.46025776039316	25.992277217792488
2	16.31298318175231	20.067465548893466	36.992623118107836	26.62692815124639
3	18.967516179728626	24.4616034203841	27.928222481423276	28.642657918464
4	12.652096069649996	15.837851121130209	35.229817900971426	36.280234908248374
5	14.62507317352119	36.53760203536507	33.52789422281569	15.309430568298046
6	34.42906270615473	34.43182188733009	17.359614115970892	13.779501290544285
7	30.08312350911067	30.314339934308045	21.407740732526552	18.194795824054736
8	28.4142005003852	32.456730733036714	19.95733927572128	19.171729490856805
9	27.285708692915634	15.105919719247243	18.5539446224022	39.05442696543492
10	15.99690946944386	26.880235916523542	31.478128070260876	25.644726543771725
11	36.96584125186322	21.27924484159677	21.834910010658447	19.92000389588156
12	24.600060353090687	23.739049704937656	28.68976051070163	22.971129431270032
13	29.779743821847088	19.64211571645387	24.954601753072698	25.62353870862634
14	23.986588765832078	19.734827888659446	24.73035195452471	31.548231390983766
15	25.750156627764078	26.62526405834585	22.851438565771613	24.773140748118458
16	26.305810277810828	25.311904034007366	24.024630039873717	24.35765564830809
17	24.37638945510265	25.500454867686724	25.78793526218654	24.33522041502409
18	25.573845229989978	24.063792268930516	26.694121262148208	23.668241238931298
19	25.207495443384996	26.201766410090325	24.894231300304778	23.696506846219894
20	25.19018932831149	23.982807778370756	26.149557869116407	24.67744502420135
21	26.24994728079354	24.85811002146927	23.850871925466087	25.0410707722711
22	25.13210480800232	25.096900295396495	24.48883148137174	25.28216341522944
23	23.783244998029087	24.7147702821792	25.02489373400989	26.477090985781825
24	24.214508329287145	25.98967557028641	24.757492391745878	25.038323708680565
25	25.1436260751244	24.527798838137606	24.478793751851498	25.8497813348865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	333.0
2	714.0
3	1095.0
4	1095.0
5	1095.0
6	2806.0
7	4517.0
8	4517.0
9	4517.0
10	4458.5
11	4400.0
12	4400.0
13	4400.0
14	5015.0
15	5630.0
16	5630.0
17	5630.0
18	9048.5
19	12467.0
20	12467.0
21	12467.0
22	20003.0
23	27539.0
24	27539.0
25	27539.0
26	41025.0
27	54511.0
28	54511.0
29	54511.0
30	67423.0
31	80335.0
32	80335.0
33	80335.0
34	100760.0
35	121185.0
36	121185.0
37	121185.0
38	137869.5
39	154554.0
40	154554.0
41	154554.0
42	181566.0
43	208578.0
44	208578.0
45	208578.0
46	256851.5
47	305125.0
48	305125.0
49	305125.0
50	287865.0
51	270605.0
52	270605.0
53	270605.0
54	245942.5
55	221280.0
56	221280.0
57	221280.0
58	203684.0
59	186088.0
60	186088.0
61	186088.0
62	164389.0
63	142690.0
64	142690.0
65	142690.0
66	117386.0
67	92082.0
68	92082.0
69	92082.0
70	69344.0
71	46606.0
72	46606.0
73	46606.0
74	36106.5
75	25607.0
76	25607.0
77	25607.0
78	21253.5
79	16900.0
80	16900.0
81	16900.0
82	11832.0
83	6764.0
84	6764.0
85	6764.0
86	4942.5
87	3121.0
88	3121.0
89	3121.0
90	2175.5
91	1230.0
92	1230.0
93	1230.0
94	723.5
95	217.0
96	217.0
97	217.0
98	514.0
99	811.0
100	811.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008524422470377633
2	0.0033596253265605956
3	0.005415515451769319
4	0.015043098477136998
5	0.036153580006719255
6	0.04638288697117241
7	0.06679135723848827
8	0.09050930917077427
9	0.09587468096095314
10	0.11929177092369637
11	0.12149809203367648
12	0.13323170884584334
13	0.12300240188139018
14	0.12571015960727486
15	0.11462841039578393
16	0.11187050900830882
17	0.12445656806751343
18	0.13127610604381554
19	0.13338213983061473
20	0.1223003906191238
21	0.12967150887292092
22	0.1528378805277119
23	0.14065297076123093
24	0.1345354440471952
25	0.13237926659880558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1994270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.15809534130044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12368848260411	40.47817199148416
2	12.733033879148445	13.027955223469693
3	3.7210762286441073	5.710895174316658
4	1.4981418691776387	3.065683383127347
5	0.7739580300016711	1.9797109344495274
6	0.4898860977423903	1.5036983816809695
7	0.3117016932928084	1.1162245460462794
8	0.21360510999763682	0.8742104466118457
9	0.1538736588258127	0.7084694977853093
>10	0.7940327741919125	7.4335118122881125
>50	0.08115280364270856	2.94033777761933
>100	0.08542699704730981	9.458251946950151
>500	0.014236752279376815	5.022931934198865
>1k	0.005989254407186109	5.421350895219518
>5k	0.0	0.0
>10k+	1.9636899695692157E-4	1.2585960547522095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13766	0.6902776454542263	No Hit
TCCATGTACTCTGCGTTGATACCAC	11291	0.566172083017846	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4990	0.2502168713363787	No Hit
CATGTACTCTGCGTTGATACCACTG	4235	0.21235840683558396	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3408	0.17088959870027629	No Hit
CCCATGTACTCTGCGTTGATACCAC	3375	0.16923485786779122	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3337	0.16732939872735386	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3321	0.16652710014190658	No Hit
GCGTTGATACCACTGCTTCCATGTA	3056	0.15323902982043555	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2833	0.1420569932857637	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2529	0.1268133201622649	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2422	0.12144794837208603	No Hit
GTATCAACGCAGAGTACATGGAAGC	2337	0.11718573713689721	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2122	0.10640484989494904	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2075	0.10404809780019757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.014366159045666E-5	0.0
5	0.0	0.0	0.0	1.0028732318091332E-4	0.0
6	0.0	0.0	0.0	1.0028732318091332E-4	0.0
7	0.0	0.0	0.0	2.0057464636182663E-4	0.0
8	0.0	0.0	0.0	2.0057464636182663E-4	0.0
9	0.0	0.0	0.0	2.507183079522833E-4	0.0
10	0.0	0.0	0.0	3.510056311331966E-4	0.0
11	0.0	0.0	0.0	3.510056311331966E-4	0.0
12	0.0	0.0	0.0	4.0114929272365326E-4	1.5043098477136998E-4
13	0.0	0.0	0.0	4.0114929272365326E-4	1.5043098477136998E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCG	55	1.9618406E-4	13.8171425	4
AATCGTA	105	1.369699E-9	13.572451	13
GTCCTAA	210	0.0	12.658085	1
CGCCAGT	325	0.0	12.571125	18
CGACCAT	325	0.0	12.570809	10
ACGGTAT	320	0.0	12.470316	9
ACAGTCG	85	3.9393562E-6	12.295661	8
TCGGCAA	70	1.09043096E-4	12.215206	13
TCGCGTA	150	1.4551915E-11	12.034844	9
CGAACGA	135	3.7107384E-10	11.963863	16
TCGTTCA	145	9.640644E-11	11.793992	16
CCGACCA	350	0.0	11.672894	9
CGTCGTA	355	0.0	11.508487	10
CGAGCCG	440	0.0	11.443757	15
ATCGCCA	325	0.0	11.400859	16
TTTTCGG	285	0.0	11.3341875	16
TTTGCGC	245	0.0	11.24631	17
AACGGAC	110	4.9670416E-7	11.227837	15
GTAATAC	110	4.9896516E-7	11.223891	3
GACGGTA	360	0.0	11.084725	8
>>END_MODULE