FastQCFastQC Report
Thu 2 Feb 2017
SRR4062373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994270
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52570.26360522898103067No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45440.22785279826703508No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20240.10149077105908427No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20150.10103947810477018No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20140.10098933444317972No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20140.10098933444317972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA1158.780262E-921.04332444
TACCGTC4950.018.2220887
ATACCGT5300.017.8489236
ACCGTCG5000.017.1598728
TGGTATA6250.016.89587644
CGTCGTA5250.016.7617810
GTCGTAG5000.016.71987511
GTATAGG2251.8189894E-1216.627521
CGAGCCG7700.016.57130615
GTCGTAT1602.1316737E-816.49987643
CCGTCGT5400.015.888779
TCTATAC2500.015.8398823
GTATAGT1401.5821297E-615.7192921
CCGATAA4500.015.6443289
GTGTAAT2700.015.4864141
CGATAAC4700.015.44669410
AGGCCCG8750.015.33702910
GTCCTAA3450.015.3092251
GTAATAC1756.416121E-815.0856033
GACTATA2651.8189894E-1214.9481571
Produced by FastQC (version 0.11.5)