##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994270
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256481318978874	32.0	32.0	32.0	32.0	32.0
2	31.403523093663345	32.0	32.0	32.0	32.0	32.0
3	31.502783474654887	32.0	32.0	32.0	32.0	32.0
4	31.607322478902056	32.0	32.0	32.0	32.0	32.0
5	31.548010550226397	32.0	32.0	32.0	32.0	32.0
6	35.132155625868116	36.0	36.0	36.0	36.0	36.0
7	35.15063908096697	36.0	36.0	36.0	36.0	36.0
8	35.09157787059927	36.0	36.0	36.0	36.0	36.0
9	35.214807423267665	36.0	36.0	36.0	36.0	36.0
10	35.09319751086864	36.0	36.0	36.0	36.0	36.0
11	35.224545322348526	36.0	36.0	36.0	36.0	36.0
12	35.13347139554825	36.0	36.0	36.0	36.0	36.0
13	35.17220687269026	36.0	36.0	36.0	36.0	36.0
14	35.13404253185376	36.0	36.0	36.0	36.0	36.0
15	35.094744442828706	36.0	36.0	36.0	36.0	36.0
16	35.09551715665381	36.0	36.0	36.0	36.0	36.0
17	35.066473446424006	36.0	36.0	36.0	36.0	36.0
18	35.07498182292267	36.0	36.0	36.0	36.0	36.0
19	35.05837474364053	36.0	36.0	36.0	36.0	36.0
20	35.05164044988893	36.0	36.0	36.0	36.0	36.0
21	35.02713323672321	36.0	36.0	36.0	36.0	36.0
22	34.996933715093746	36.0	36.0	36.0	36.0	36.0
23	34.94010088904712	36.0	36.0	36.0	32.0	36.0
24	34.920008323847824	36.0	36.0	36.0	32.0	36.0
25	34.886164862330574	36.0	36.0	36.0	32.0	36.0
26	34.82274817351713	36.0	36.0	36.0	32.0	36.0
27	34.79477803908197	36.0	36.0	36.0	32.0	36.0
28	34.76572630586631	36.0	36.0	36.0	32.0	36.0
29	34.734273192697074	36.0	36.0	36.0	32.0	36.0
30	34.71846941487361	36.0	36.0	36.0	32.0	36.0
31	34.705464656240125	36.0	36.0	36.0	32.0	36.0
32	34.66256775662272	36.0	36.0	36.0	32.0	36.0
33	34.63351652484368	36.0	36.0	36.0	32.0	36.0
34	34.60868939511701	36.0	36.0	36.0	32.0	36.0
35	34.548655899151065	36.0	36.0	36.0	32.0	36.0
36	34.524594463136886	36.0	36.0	36.0	32.0	36.0
37	34.48516750490154	36.0	36.0	36.0	32.0	36.0
38	34.42780967471807	36.0	36.0	36.0	32.0	36.0
39	34.345579585512496	36.0	36.0	36.0	32.0	36.0
40	34.293642285146944	36.0	36.0	36.0	32.0	36.0
41	34.281332016226486	36.0	36.0	36.0	32.0	36.0
42	34.183005310213765	36.0	36.0	36.0	32.0	36.0
43	34.20102694218937	36.0	36.0	36.0	32.0	36.0
44	34.17800247709688	36.0	36.0	36.0	32.0	36.0
45	34.059816373911254	36.0	36.0	36.0	32.0	36.0
46	34.14085755690052	36.0	36.0	36.0	32.0	36.0
47	34.06441153905941	36.0	36.0	36.0	32.0	36.0
48	34.07099389751639	36.0	36.0	36.0	32.0	36.0
49	34.088890671774635	36.0	36.0	36.0	32.0	36.0
50	33.531927472207876	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	66.0
22	187.0
23	623.0
24	1756.0
25	4228.0
26	8826.0
27	16354.0
28	26468.0
29	37917.0
30	52226.0
31	72429.0
32	105543.0
33	155231.0
34	334393.0
35	1178012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.887136644782146	17.692410696150507	11.978980426299342	26.441472232768003
2	15.735793953021435	20.52822555167873	36.63254079609425	27.103439699205587
3	18.440656242760483	24.62494897861966	28.239067885764257	28.695326892855604
4	12.448333146965778	16.325797899679433	35.34220844049468	35.88366051286012
5	14.402914349611637	36.629593786197454	33.4731004327398	15.494391431451108
6	34.60810796580789	34.64145365125227	17.037489074900428	13.712949308039418
7	30.01569496607781	30.31284630466286	21.379953566969366	18.291505162289962
8	28.444694048448802	32.61258505618597	19.7540954835604	19.18862541180482
9	27.284871155861545	14.79072542835223	18.451964879379425	39.472438536406806
10	15.964337827876868	26.89360016447121	31.530234120755967	25.611827886895956
11	37.206346181810886	21.093883977595812	21.808030005967098	19.891739834626204
12	24.52265741348965	23.692679526844408	28.734724987088008	23.049938072577937
13	29.99277931273097	19.399880658085415	25.040390719411114	25.566949309772497
14	24.0258340144514	19.485626319405096	24.76324670180066	31.725292964342845
15	25.84509620061476	26.552021541717018	22.838783113620522	24.764099144047698
16	26.314741735070978	25.198192822436276	24.028291053869335	24.458774388623404
17	24.48003530113776	25.50888294965075	25.656706464019415	24.354375285192077
18	25.72495198744403	23.814027187893313	26.733341022028114	23.727679802634547
19	25.2984300019556	26.035240965365773	24.949680835593977	23.71664819708465
20	25.274862481008086	23.757765999588823	26.23335857230967	24.734012947093422
21	26.38098151203197	24.663761677205194	23.92900660392023	25.026250206842604
22	25.12222517512674	24.879279134720974	24.555601799154577	25.44289389099771
23	23.805352334438165	24.41540012134766	25.19403089852427	26.5852166456899
24	24.323587076975535	25.68995171165389	24.86664293200018	25.1198182793704
25	25.30585126387099	24.161974055669493	24.659950758924317	25.8722239215352
26	24.36365186258631	24.84808977721171	25.48717074418208	25.301087616019895
27	25.88771831296665	24.942861297617675	24.54722780766897	24.622192581746706
28	24.746448575167857	23.944300420705318	25.470172042902917	25.839078961223905
29	24.28888766315494	25.045204510923796	25.98248983337261	24.68341799254865
30	25.30885988356642	24.457671228068417	25.978628771430145	24.25484011693502
31	24.767258194727894	24.540759275323804	24.789521980474056	25.90246054947424
32	24.389225129997445	24.97881430297803	24.84558259413219	25.78637797289234
33	24.231222452325916	24.498789030572592	25.323802694720378	25.946185822381125
34	24.567235128643564	24.901843782436682	25.996580202279528	24.534340886640223
35	26.0435146695282	24.404518946782535	25.37133888590813	24.180627497781142
36	24.801105166301454	25.173371709949006	25.05302692213191	24.972496201617634
37	25.939867721020725	24.921700672426503	24.926765182247138	24.211666424305637
38	24.75131251034213	24.80606938879891	24.938298224412943	25.50431987644602
39	24.972646632602405	24.65468567445732	25.046207384155604	25.32646030878467
40	25.62516610087902	24.82056090699855	24.87200830379036	24.68226468833207
41	24.041579124190807	25.03642937014547	25.759801832249394	25.162189673414332
42	26.217763893555034	25.169961940960857	24.979616601563478	23.632657563920635
43	25.110140552683436	23.84747301017415	25.02218857025378	26.020197866888633
44	24.747652023046026	24.664212970159507	25.55767273237826	25.030462274416205
45	25.06215306854137	24.793533473401293	25.207619830815286	24.93669362724205
46	24.455890582409186	24.549809754757135	25.921440849474592	25.07285881335909
47	25.08266182613187	24.15851414301975	26.166717646055947	24.59210638479243
48	25.257964067052104	26.134274697006926	24.236086387500187	24.371674848440783
49	24.261208361957006	24.997718463397632	25.893384546726374	24.847688627918988
50	24.118962888156013	26.364497467493102	24.367274424864448	25.149265219486438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	34.0
2	41.0
3	180.5
4	320.0
5	335.5
6	351.0
7	324.0
8	297.0
9	407.0
10	517.0
11	766.0
12	1015.0
13	1772.5
14	2530.0
15	3298.5
16	4067.0
17	4843.0
18	5619.0
19	5942.5
20	6266.0
21	7101.0
22	7936.0
23	8478.0
24	9020.0
25	10318.5
26	11617.0
27	14421.0
28	17225.0
29	20118.0
30	23011.0
31	25720.5
32	28430.0
33	33433.0
34	38436.0
35	44603.0
36	50770.0
37	61143.0
38	71516.0
39	77886.5
40	84257.0
41	93583.5
42	102910.0
43	107645.0
44	112380.0
45	128150.5
46	143921.0
47	156433.5
48	168946.0
49	167228.0
50	165510.0
51	153801.5
52	142093.0
53	138312.5
54	134532.0
55	135534.0
56	136536.0
57	132946.5
58	129357.0
59	118633.5
60	107910.0
61	94836.5
62	81763.0
63	71072.5
64	60382.0
65	51691.5
66	43001.0
67	37155.0
68	31309.0
69	28976.5
70	26644.0
71	20755.0
72	14866.0
73	13310.0
74	11754.0
75	8427.5
76	5101.0
77	4763.5
78	4426.0
79	3769.0
80	3112.0
81	2476.0
82	1840.0
83	1585.0
84	1330.0
85	1094.0
86	858.0
87	612.0
88	366.0
89	227.5
90	89.0
91	65.5
92	42.0
93	26.0
94	10.0
95	7.5
96	5.0
97	5.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015995828047355675
2	0.004212067573598359
3	4.011492927236533E-4
4	1.5043098477136998E-4
5	0.0
6	4.5129295431410994E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0028732318091333E-4
47	0.0
48	0.0
49	0.0
50	5.0143661590456665E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1994270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.353047374832386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16002667000905	43.02588679787997
2	12.926665959011654	14.052073745375871
3	3.649575291620045	5.950966161703261
4	1.4663645212041292	3.1880552115912577
5	0.7434272843634189	2.0203769203373945
6	0.46110774515775305	1.5037566670476894
7	0.29798868666325	1.1337615242360197
8	0.19998535634428266	0.8695850838122824
9	0.15079398045109063	0.737650112696791
>10	0.7755956853174043	7.668452651005257
>50	0.07557100255158619	2.9178233769211284
>100	0.0778798961485146	9.234137333335068
>500	0.010846276391902816	4.1375304320328485
>1k	0.0040789415490916575	3.2950593996564352
>5k	9.27032170248104E-5	0.264884582368589
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5257	0.26360522898103067	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4544	0.22785279826703508	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2024	0.10149077105908427	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2015	0.10103947810477018	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2014	0.10098933444317972	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2014	0.10098933444317972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.014366159045666E-5	0.0
6	0.0	0.0	0.0	5.014366159045666E-5	5.014366159045666E-5
7	0.0	0.0	0.0	1.5043098477136998E-4	5.014366159045666E-5
8	0.0	0.0	0.0	3.0086196954273996E-4	5.014366159045666E-5
9	0.0	0.0	0.0	4.0114929272365326E-4	5.014366159045666E-5
10	0.0	0.0	0.0	4.5129295431410994E-4	5.014366159045666E-5
11	0.0	0.0	0.0	4.5129295431410994E-4	5.014366159045666E-5
12	5.014366159045666E-5	0.0	0.0	4.5129295431410994E-4	1.5043098477136998E-4
13	5.014366159045666E-5	0.0	0.0	4.5129295431410994E-4	1.5043098477136998E-4
14	5.014366159045666E-5	0.0	0.0	4.5129295431410994E-4	1.5043098477136998E-4
15	5.014366159045666E-5	0.0	0.0	5.014366159045666E-4	2.0057464636182663E-4
16	5.014366159045666E-5	0.0	0.0	5.515802774950232E-4	2.0057464636182663E-4
17	5.014366159045666E-5	0.0	0.0	6.518676006759366E-4	2.0057464636182663E-4
18	5.014366159045666E-5	0.0	0.0	7.521549238568498E-4	2.507183079522833E-4
19	5.014366159045666E-5	0.0	0.0	0.0010028732318091331	2.507183079522833E-4
20	5.014366159045666E-5	0.0	0.0	0.0012535915397614164	2.507183079522833E-4
21	5.014366159045666E-5	0.0	0.0	0.0018553154788468963	2.507183079522833E-4
22	5.014366159045666E-5	0.0	0.0	0.0026074704027037464	2.507183079522833E-4
23	5.014366159045666E-5	0.0	0.0	0.004161923912007903	2.507183079522833E-4
24	5.014366159045666E-5	0.0	0.0	0.005766521082902516	2.507183079522833E-4
25	5.014366159045666E-5	0.0	0.0	0.007070256284254389	2.507183079522833E-4
26	5.014366159045666E-5	0.0	0.0	0.009076002747872656	2.507183079522833E-4
27	5.014366159045666E-5	0.0	0.0	0.011432754842624118	2.507183079522833E-4
28	5.014366159045666E-5	0.0	0.0	0.01664769564803161	2.507183079522833E-4
29	5.014366159045666E-5	0.0	0.0	0.024269532209781022	2.507183079522833E-4
30	5.014366159045666E-5	0.0	0.0	0.037708033516023406	2.507183079522833E-4
31	5.014366159045666E-5	0.0	0.0	0.067092219208031	2.507183079522833E-4
32	5.014366159045666E-5	0.0	0.0	0.10234321330612203	2.507183079522833E-4
33	5.014366159045666E-5	0.0	0.0	0.13608989755649936	2.507183079522833E-4
34	5.014366159045666E-5	0.0	0.0	0.1784111479388448	3.0086196954273996E-4
35	5.014366159045666E-5	0.0	0.0	0.2239917363245699	3.0086196954273996E-4
36	5.014366159045666E-5	0.0	0.0	0.2908332372246486	3.0086196954273996E-4
37	5.014366159045666E-5	0.0	0.0	0.38851309000285816	3.0086196954273996E-4
38	5.014366159045666E-5	0.0	0.0	0.5165800017048845	3.510056311331966E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	115	8.780262E-9	21.043324	44
TACCGTC	495	0.0	18.222088	7
ATACCGT	530	0.0	17.848923	6
ACCGTCG	500	0.0	17.159872	8
TGGTATA	625	0.0	16.895876	44
CGTCGTA	525	0.0	16.76178	10
GTCGTAG	500	0.0	16.719875	11
GTATAGG	225	1.8189894E-12	16.62752	1
CGAGCCG	770	0.0	16.571306	15
GTCGTAT	160	2.1316737E-8	16.499876	43
CCGTCGT	540	0.0	15.88877	9
TCTATAC	250	0.0	15.839882	3
GTATAGT	140	1.5821297E-6	15.719292	1
CCGATAA	450	0.0	15.644328	9
GTGTAAT	270	0.0	15.486414	1
CGATAAC	470	0.0	15.446694	10
AGGCCCG	875	0.0	15.337029	10
GTCCTAA	345	0.0	15.309225	1
GTAATAC	175	6.416121E-8	15.085603	3
GACTATA	265	1.8189894E-12	14.948157	1
>>END_MODULE