FastQCFastQC Report
Thu 2 Feb 2017
SRR4062372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612838
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57970.1604555753676196No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG56810.15724480311599912No Hit
GAATAGGACCGCGGTTCTATTTTGT53250.14739105379206044No Hit
GTCTTGCGCCGGTCCAAGAATTTCA52660.14575798859511555No Hit
CTATTGGAGCTGGAATTACCGCGGC52100.14420796061157462No Hit
GTATCTGATCGTCTTCGAACCTCCG51130.14152309071151267No Hit
GATTAAGAGGGACGGCCGGGGGCAT50910.14091415114655018No Hit
GCGCAAGACGGACCAGAGCGAAAGC49120.13595959741344615No Hit
GTACATGGAAGCAGTGGTATCAACG49120.13595959741344615No Hit
GAACTACGACGGTATCTGATCGTCT47470.1313925506762274No Hit
GAATAACGCCGCCGCATCGCCAGTC44890.1242513503234853No Hit
GTCCTATTCCATTATTCCTAGCTGC44760.12389152239873473No Hit
ATCAGATACCGTCGTAGTTCCGACC44640.12355937354511884No Hit
GGGTAGGCACACGCTGAGCCAGTCA44410.12292275490902167No Hit
TCGTAGTTCCGACCATAAACGATGC43390.12009948965328641No Hit
TCCATGTACTCTGCGTTGATACCAC42750.11832802910066824No Hit
GTACATGGGGAATAATTGCAATCCC39630.10969215890665454No Hit
CTTTAATATACGCTATTGGAGCTGG37400.10351972604362553No Hit
TATCAACGCAGAGTACTTTTTTTTT37270.10315989811887497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG5300.012.5477097
GAACCGC6050.012.0915986
CGTCGTA8150.012.00648510
CGACCAT8900.011.95540610
CGAACGA4400.011.87715616
CCGACCA8650.011.8616189
GTATTAC1459.822543E-1111.7869141
GAGGTTA3400.011.7340115
CCGTCGT8350.011.7189059
GACCATA9150.011.52492611
AGAACCG6050.011.4615565
CGGTCCA10050.011.34361610
TAGAACC6750.011.2577164
GGTTCCG4300.011.0469878
GCGCGCT2250.010.97885915
GTCGTAG9000.010.872539511
ACCGTCG9100.010.857618
GCGTTAT5250.010.8514441
ACGGTAT9050.010.812479
GTATTAG6950.010.7928931
Produced by FastQC (version 0.11.5)