##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062372_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3612838
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21983714741707	32.0	32.0	32.0	32.0	32.0
2	30.839424020672944	32.0	32.0	32.0	32.0	32.0
3	30.82699805526846	32.0	32.0	32.0	32.0	32.0
4	30.908481919200362	32.0	32.0	32.0	32.0	32.0
5	30.676383496852058	32.0	32.0	32.0	32.0	32.0
6	34.35290981771117	36.0	36.0	36.0	32.0	36.0
7	34.24032187438241	36.0	36.0	36.0	32.0	36.0
8	34.238777935794516	36.0	36.0	36.0	32.0	36.0
9	34.41567155792759	36.0	36.0	36.0	32.0	36.0
10	34.05271894283663	36.0	36.0	36.0	32.0	36.0
11	34.38483984059069	36.0	36.0	36.0	32.0	36.0
12	34.14415813828353	36.0	36.0	36.0	32.0	36.0
13	34.28053873436894	36.0	36.0	36.0	32.0	36.0
14	34.17983452344113	36.0	36.0	36.0	32.0	36.0
15	34.10715177375791	36.0	36.0	36.0	32.0	36.0
16	34.108261981301126	36.0	36.0	36.0	32.0	36.0
17	34.02464599851972	36.0	36.0	36.0	32.0	36.0
18	34.03946869469375	36.0	36.0	36.0	32.0	36.0
19	34.02186978768491	36.0	36.0	36.0	32.0	36.0
20	34.02713794529398	36.0	36.0	36.0	32.0	36.0
21	34.00372864767255	36.0	36.0	36.0	32.0	36.0
22	33.95081539775656	36.0	36.0	36.0	32.0	36.0
23	33.91311677966186	36.0	36.0	36.0	32.0	36.0
24	33.887717910407275	36.0	36.0	36.0	32.0	36.0
25	33.42885537629974	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	14.0
4	138.0
5	334.0
6	631.0
7	145.0
8	546.0
9	651.0
10	422.0
11	115.0
12	268.0
13	180.0
14	505.0
15	603.0
16	974.0
17	1436.0
18	1942.0
19	2754.0
20	4419.0
21	6534.0
22	9934.0
23	14758.0
24	21296.0
25	30366.0
26	42747.0
27	55425.0
28	75282.0
29	100041.0
30	129156.0
31	177880.0
32	256148.0
33	372452.0
34	822668.0
35	1482073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38893559349809	17.064424771608003	11.499585194789592	26.04705444010432
2	17.26234451115628	19.628401766093624	36.50657579512826	26.602677927621826
3	19.282276983121925	23.44207547039244	27.528188945864212	29.747458600621425
4	12.938697692419062	15.234181110098216	35.00476564725463	36.822355550228096
5	15.591327734089349	35.6125706377772	32.516899211670086	16.279202416463363
6	34.93312265328372	34.34790952388469	16.77271283517249	13.9462549876591
7	31.297949899858992	29.675197463777646	20.359770873604624	18.667081762758734
8	28.763870939144482	31.586775874989435	19.428581718548934	20.220771467317153
9	27.584011472304997	14.732940875295203	18.51823633258602	39.16481131981377
10	16.821564892371182	25.48224993252242	29.930809847348254	27.76537532775814
11	37.857492223384575	20.86097697844717	21.930401889942704	19.351128908225547
12	25.96497860253089	23.07318153384974	27.409435949909884	23.552403913709487
13	29.435285530239565	18.863249594645186	25.125153558185286	26.576311316929964
14	24.38874201182776	19.681027363473618	23.9636299141462	31.966600710552424
15	25.73803335199153	26.753944546356383	21.661863566080502	25.84615853557158
16	26.853110849838878	25.073451641068857	23.053909057128376	25.019528451963886
17	24.705928323163754	25.254248748917128	24.689799621668328	25.350023306250787
18	25.949460756952348	23.760117909010738	25.713720336366602	24.57670099767031
19	26.15715790649392	24.481247760265777	24.58770182551677	24.773892507723534
20	26.407097960827198	23.466602077942664	24.213483099360865	25.91281686186927
21	27.665009438137727	23.588665144407248	23.55269185775974	25.193633559695282
22	26.51545727613533	23.726844023493328	24.092555160205485	25.665143540165854
23	24.553688483014803	23.54881197015665	24.98529618011112	26.912203366717424
24	25.221082821489915	24.575884338214934	24.470841069534487	25.732191770760664
25	25.369735753571394	23.89497766882796	24.520111912221797	26.215174665378854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	362.0
2	914.5
3	1467.0
4	1467.0
5	1467.0
6	3433.0
7	5399.0
8	5399.0
9	5399.0
10	5496.5
11	5594.0
12	5594.0
13	5594.0
14	6396.5
15	7199.0
16	7199.0
17	7199.0
18	11980.0
19	16761.0
20	16761.0
21	16761.0
22	29217.5
23	41674.0
24	41674.0
25	41674.0
26	65397.5
27	89121.0
28	89121.0
29	89121.0
30	111321.5
31	133522.0
32	133522.0
33	133522.0
34	172056.0
35	210590.0
36	210590.0
37	210590.0
38	240969.5
39	271349.0
40	271349.0
41	271349.0
42	315239.0
43	359129.0
44	359129.0
45	359129.0
46	416338.5
47	473548.0
48	473548.0
49	473548.0
50	479002.0
51	484456.0
52	484456.0
53	484456.0
54	449603.5
55	414751.0
56	414751.0
57	414751.0
58	389974.0
59	365197.0
60	365197.0
61	365197.0
62	328750.0
63	292303.0
64	292303.0
65	292303.0
66	245029.5
67	197756.0
68	197756.0
69	197756.0
70	151078.0
71	104400.0
72	104400.0
73	104400.0
74	83706.5
75	63013.0
76	63013.0
77	63013.0
78	53349.5
79	43686.0
80	43686.0
81	43686.0
82	30869.5
83	18053.0
84	18053.0
85	18053.0
86	13362.0
87	8671.0
88	8671.0
89	8671.0
90	5800.0
91	2929.0
92	2929.0
93	2929.0
94	1735.5
95	542.0
96	542.0
97	542.0
98	954.0
99	1366.0
100	1366.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008276042269263111
2	0.003293809465024449
3	0.004511688594949455
4	0.014586870487965416
5	0.03540153198122916
6	0.04636244415055422
7	0.06723246378608728
8	0.08920964626700671
9	0.09549279541457435
10	0.11713782904187789
11	0.11799588024705232
12	0.13011931340403307
13	0.1182173128161296
14	0.12117897342753814
15	0.11232167066444716
16	0.1107439636097716
17	0.1207914664316529
18	0.12820945749574159
19	0.13034074597311035
20	0.12081914550278755
21	0.12746212257510578
22	0.14797231428588828
23	0.13651317883613934
24	0.132887220517499
25	0.12879071798956943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3612838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.06832129901943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59742082145911	30.63590846373845
2	14.290681532627103	11.737886015277866
3	4.704569589913716	5.796263264765178
4	2.057965109912315	3.380686894242032
5	1.1032134062112846	2.265356131383534
6	0.6405838511132307	1.578462204988883
7	0.43533955204890107	1.2515065218400825
8	0.31349774811490394	1.0299860996881551
9	0.2419516636815397	0.8942893796615188
>10	1.3706487708437718	10.437977844145285
>50	0.10907378246609993	3.127406522542428
>100	0.09813522535532723	8.680679189731535
>500	0.020254614549892806	5.889863371615597
>1k	0.016190143402221958	12.2509447456348
>5k	4.741883004834883E-4	1.042783350744681
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5797	0.1604555753676196	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5681	0.15724480311599912	No Hit
GAATAGGACCGCGGTTCTATTTTGT	5325	0.14739105379206044	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	5266	0.14575798859511555	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5210	0.14420796061157462	No Hit
GTATCTGATCGTCTTCGAACCTCCG	5113	0.14152309071151267	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	5091	0.14091415114655018	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4912	0.13595959741344615	No Hit
GTACATGGAAGCAGTGGTATCAACG	4912	0.13595959741344615	No Hit
GAACTACGACGGTATCTGATCGTCT	4747	0.1313925506762274	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4489	0.1242513503234853	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4476	0.12389152239873473	No Hit
ATCAGATACCGTCGTAGTTCCGACC	4464	0.12355937354511884	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4441	0.12292275490902167	No Hit
TCGTAGTTCCGACCATAAACGATGC	4339	0.12009948965328641	No Hit
TCCATGTACTCTGCGTTGATACCAC	4275	0.11832802910066824	No Hit
GTACATGGGGAATAATTGCAATCCC	3963	0.10969215890665454	No Hit
CTTTAATATACGCTATTGGAGCTGG	3740	0.10351972604362553	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3727	0.10315989811887497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.535814226931847E-5
2	0.0	0.0	0.0	0.0	5.535814226931847E-5
3	0.0	0.0	0.0	0.0	5.535814226931847E-5
4	0.0	0.0	0.0	5.535814226931847E-5	5.535814226931847E-5
5	0.0	0.0	0.0	5.535814226931847E-5	5.535814226931847E-5
6	0.0	0.0	0.0	5.535814226931847E-5	5.535814226931847E-5
7	0.0	2.7679071134659236E-5	0.0	8.30372134039777E-5	5.535814226931847E-5
8	0.0	2.7679071134659236E-5	0.0	8.30372134039777E-5	5.535814226931847E-5
9	0.0	2.7679071134659236E-5	0.0	1.1071628453863694E-4	5.535814226931847E-5
10	0.0	2.7679071134659236E-5	0.0	1.1071628453863694E-4	5.535814226931847E-5
11	0.0	2.7679071134659236E-5	0.0	1.1071628453863694E-4	5.535814226931847E-5
12	0.0	2.7679071134659236E-5	0.0	1.1071628453863694E-4	1.1071628453863694E-4
13	0.0	2.7679071134659236E-5	0.0	1.1071628453863694E-4	2.214325690772739E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	530	0.0	12.547709	7
GAACCGC	605	0.0	12.091598	6
CGTCGTA	815	0.0	12.006485	10
CGACCAT	890	0.0	11.955406	10
CGAACGA	440	0.0	11.877156	16
CCGACCA	865	0.0	11.861618	9
GTATTAC	145	9.822543E-11	11.786914	1
GAGGTTA	340	0.0	11.734011	5
CCGTCGT	835	0.0	11.718905	9
GACCATA	915	0.0	11.524926	11
AGAACCG	605	0.0	11.461556	5
CGGTCCA	1005	0.0	11.343616	10
TAGAACC	675	0.0	11.257716	4
GGTTCCG	430	0.0	11.046987	8
GCGCGCT	225	0.0	10.978859	15
GTCGTAG	900	0.0	10.8725395	11
ACCGTCG	910	0.0	10.85761	8
GCGTTAT	525	0.0	10.851444	1
ACGGTAT	905	0.0	10.81247	9
GTATTAG	695	0.0	10.792893	1
>>END_MODULE