FastQCFastQC Report
Thu 2 Feb 2017
SRR4062372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612838
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC54010.14949466319829452No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT53520.14813838871269625No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA53330.1476124863611377No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA53330.1476124863611377No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA49670.13748194632585242No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG48260.13357919729586545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT47660.1319184530277859No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC46880.12975948547928248No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA45990.1272960481482978No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT45700.12649335508539272No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC43100.1192967965903813No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG42600.11791284303364835No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT37480.1037411586127028No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36810.10188666084668065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG7850.021.2992694
AGGACGT9350.017.4116885
GTATCAA81900.017.3825421
TAACGGC4100.017.17065636
TTAACGG4150.016.43366435
TGTAGGA11950.016.2012142
CGCAATA18000.016.13326336
GGACGTG9550.016.1255846
GTCCTAC11700.015.7974261
ATACGAA18550.015.77351640
GTATTAC3100.015.6154281
TCTAGCG19250.015.314218528
TACGACG14800.015.3107435
TATGCGT1158.22506E-515.3042839
TAGCGGC18900.015.2486130
ATACCGT14600.015.21911056
ACCGTCG14050.015.1885458
AACGAAT3200.015.12493431
CGACGGT15350.015.0487947
TACGAAT19600.015.04074941
Produced by FastQC (version 0.11.5)