##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3612838
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32503837703213	32.0	32.0	32.0	32.0	32.0
2	31.439409406123385	32.0	32.0	32.0	32.0	32.0
3	31.512671478765448	32.0	32.0	32.0	32.0	32.0
4	31.61238173424881	32.0	32.0	32.0	32.0	32.0
5	31.54151057977136	32.0	32.0	32.0	32.0	32.0
6	35.150264141375835	36.0	36.0	36.0	36.0	36.0
7	35.159496218762094	36.0	36.0	36.0	36.0	36.0
8	35.101386500031275	36.0	36.0	36.0	36.0	36.0
9	35.2277439508774	36.0	36.0	36.0	36.0	36.0
10	35.101956965687364	36.0	36.0	36.0	36.0	36.0
11	35.237586628572885	36.0	36.0	36.0	36.0	36.0
12	35.138645851267064	36.0	36.0	36.0	36.0	36.0
13	35.18505922490851	36.0	36.0	36.0	36.0	36.0
14	35.14883645488671	36.0	36.0	36.0	36.0	36.0
15	35.10672247136462	36.0	36.0	36.0	36.0	36.0
16	35.11667282064681	36.0	36.0	36.0	36.0	36.0
17	35.08243187211826	36.0	36.0	36.0	36.0	36.0
18	35.10263980837225	36.0	36.0	36.0	36.0	36.0
19	35.085973686060655	36.0	36.0	36.0	36.0	36.0
20	35.078950398550944	36.0	36.0	36.0	36.0	36.0
21	35.07437006585958	36.0	36.0	36.0	36.0	36.0
22	35.056305320083545	36.0	36.0	36.0	36.0	36.0
23	35.010399857397424	36.0	36.0	36.0	36.0	36.0
24	34.988868861543196	36.0	36.0	36.0	36.0	36.0
25	34.951936953718935	36.0	36.0	36.0	32.0	36.0
26	34.912025670677735	36.0	36.0	36.0	32.0	36.0
27	34.89569031326619	36.0	36.0	36.0	32.0	36.0
28	34.87819963142549	36.0	36.0	36.0	32.0	36.0
29	34.85120174223145	36.0	36.0	36.0	32.0	36.0
30	34.84336579719323	36.0	36.0	36.0	32.0	36.0
31	34.84492468247954	36.0	36.0	36.0	32.0	36.0
32	34.80517864349301	36.0	36.0	36.0	32.0	36.0
33	34.78815684511733	36.0	36.0	36.0	32.0	36.0
34	34.77738110593389	36.0	36.0	36.0	32.0	36.0
35	34.74915205165579	36.0	36.0	36.0	32.0	36.0
36	34.73109118094971	36.0	36.0	36.0	32.0	36.0
37	34.71303252456933	36.0	36.0	36.0	32.0	36.0
38	34.6830807249038	36.0	36.0	36.0	32.0	36.0
39	34.66611649899608	36.0	36.0	36.0	32.0	36.0
40	34.661157516611595	36.0	36.0	36.0	32.0	36.0
41	34.6290500708861	36.0	36.0	36.0	32.0	36.0
42	34.59247549987018	36.0	36.0	36.0	32.0	36.0
43	34.59543383899306	36.0	36.0	36.0	32.0	36.0
44	34.55891075104945	36.0	36.0	36.0	32.0	36.0
45	34.51465745211936	36.0	36.0	36.0	32.0	36.0
46	34.52958173048446	36.0	36.0	36.0	32.0	36.0
47	34.49823961107584	36.0	36.0	36.0	32.0	36.0
48	34.46270355880889	36.0	36.0	36.0	32.0	36.0
49	34.46574770305228	36.0	36.0	36.0	32.0	36.0
50	33.94886540719512	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	27.0
21	103.0
22	308.0
23	923.0
24	2394.0
25	5581.0
26	11345.0
27	21122.0
28	35376.0
29	53437.0
30	77953.0
31	112726.0
32	167252.0
33	277434.0
34	625695.0
35	2221155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79546848159944	17.68919968274665	12.012108779695787	26.50322305595812
2	16.5729049487586	20.132537844229695	36.201498932655426	27.093058274356274
3	18.650221503176887	23.72460256060717	27.938989403000154	29.686186533215793
4	12.643467863143298	15.716294428467434	35.16437252790748	36.47586518048178
5	15.281421419947423	35.8329656630051	32.48828206523514	16.39733085181234
6	35.16512899093559	34.53287909805437	16.50786146635917	13.79413044465087
7	31.25098952734085	29.711506417672982	20.36425124196061	18.673252813025556
8	28.812446060410124	31.63834082790316	19.246033173920335	20.303179937766377
9	27.660000254647453	14.492817004249844	18.369021805018658	39.478160936084045
10	16.789930796786347	25.57014734676728	29.877481359529547	27.76244049691683
11	38.078651741373406	20.72237947010079	21.85727674476409	19.341692043761718
12	25.977306483158113	23.03582391460674	27.343379359938087	23.64349024229705
13	29.64132352460863	18.695330374625158	25.097305774573897	26.56604032619232
14	24.44059213283297	19.582195492850772	23.903645831891716	32.073566542424544
15	25.91984473148256	26.62723875247105	21.574950219190566	25.877966296855824
16	26.97394679750379	25.100654942181187	22.883063121014562	25.04233513930046
17	24.863002437419006	25.26886065746651	24.47895532542561	25.389181579688874
18	26.09258981443397	23.70211451496026	25.61833660961272	24.586959060993046
19	26.226694914081396	24.46602919920572	24.5651202738678	24.74215561284508
20	26.54746766946096	23.26428696775222	24.18198657122185	26.006258791564967
21	27.804900192037397	23.59303129561857	23.449404595500823	25.152663916843217
22	26.59800965335285	23.71119325029243	24.078882031245243	25.611915065109482
23	24.638469812374648	23.368249558934	24.977372359347417	27.015908269343935
24	25.383119863110387	24.473474869340944	24.37463290631908	25.76877236122959
25	25.56718568615587	23.680497160404094	24.522909690387447	26.22940746305259
26	25.18546361613778	24.786386768518266	24.947368246237446	25.080781369106504
27	26.273555581512376	23.88676159849957	24.114255884155337	25.72542693583272
28	25.10751935182258	23.845796573220277	25.04665307439747	26.00003100055967
29	24.983544792210445	24.126406996383455	25.01484982166374	25.87519838974236
30	24.92406246834206	24.30147712131017	25.40515240373357	25.36930800661419
31	25.947717556115162	24.1099933072006	23.728520348822727	26.213768787861508
32	25.47097323489179	24.11176476775322	23.868521090621833	26.548740906733155
33	24.84105293400922	23.6966340588756	24.800143266872194	26.66216974024299
34	25.878049334069225	23.904586920310294	24.899538811316756	25.317824934303722
35	26.705764277280075	23.644680442355842	24.76703909779514	24.88251618256894
36	25.129662608730314	24.452494133420874	24.31661757322083	26.101225684627988
37	26.504841899913583	24.224335550057877	24.070716705260516	25.200105844768018
38	25.31458648298097	23.849311815254378	24.659533585508125	26.176568116256526
39	26.01791168051266	23.70388597551288	24.094022483155904	26.184179860818556
40	26.362017892858745	24.006611976512648	24.611067531951335	25.020302598677276
41	24.92583392889468	24.48139108368546	25.260086391916825	25.33268859550304
42	26.850525819314345	24.64566637086966	24.32320519215088	24.180602617665116
43	25.620108070165337	23.282527475629962	24.756299618194895	26.34106483600981
44	25.148761167813227	24.169973854349404	24.406519196266206	26.274745781571163
45	25.19996744941235	24.454708459111647	24.562407724896605	25.782916366579407
46	24.982451439756957	23.915975057793997	24.99274807131912	26.10882543112993
47	25.422569576208392	23.667190078046698	25.329374117902358	25.580866227842552
48	26.383995075339662	24.6277303327744	23.89210365922856	25.096170932657376
49	25.01067720464555	24.608804659606843	24.47566829059822	25.904849845149393
50	25.018371988550825	25.04981542206305	24.12013052346397	25.811682065922152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	33.0
2	50.0
3	139.0
4	228.0
5	270.0
6	312.0
7	315.0
8	318.0
9	456.0
10	594.0
11	846.0
12	1098.0
13	2084.5
14	3071.0
15	4167.0
16	5263.0
17	5864.0
18	6465.0
19	7081.0
20	7697.0
21	8678.0
22	9659.0
23	10505.0
24	11351.0
25	13252.0
26	15153.0
27	19423.0
28	23693.0
29	28534.5
30	33376.0
31	38157.0
32	42938.0
33	51455.5
34	59973.0
35	71568.0
36	83163.0
37	103048.5
38	122934.0
39	134957.0
40	146980.0
41	161211.0
42	175442.0
43	179354.5
44	183267.0
45	207466.0
46	231665.0
47	252323.0
48	272981.0
49	281308.5
50	289636.0
51	272301.5
52	254967.0
53	251965.0
54	248963.0
55	258394.5
56	267826.0
57	265064.5
58	262303.0
59	240156.5
60	218010.0
61	193198.5
62	168387.0
63	148597.5
64	128808.0
65	110814.5
66	92821.0
67	83040.0
68	73259.0
69	68810.0
70	64361.0
71	49896.5
72	35432.0
73	31656.0
74	27880.0
75	20005.0
76	12130.0
77	11312.0
78	10494.0
79	9159.0
80	7824.0
81	6165.0
82	4506.0
83	4047.5
84	3589.0
85	3014.5
86	2440.0
87	1759.0
88	1078.0
89	683.0
90	288.0
91	176.0
92	64.0
93	50.0
94	36.0
95	29.5
96	23.0
97	20.5
98	18.0
99	13.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016136898471506334
2	0.0037089955320443375
3	6.366186360971624E-4
4	2.214325690772739E-4
5	0.0
6	7.1965584950114E-4
7	5.535814226931847E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.660744268079554E-4
47	2.7679071134659236E-5
48	0.0
49	2.7679071134659236E-5
50	2.7679071134659236E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3612838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.163883616938385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2705011100091	33.24237650811073
2	14.189021518156833	12.532845899322256
3	4.595817730937224	6.089074781813202
4	1.9390913211623173	3.425512137217115
5	1.0065957565674384	2.2227588921174193
6	0.5942054908432639	1.574545328528859
7	0.42687304506045043	1.3196660036880479
8	0.28899899689830777	1.021065445154304
9	0.2282139860851498	0.9070934329099938
>10	1.2482254588689483	10.168787541036673
>50	0.09830903833177077	3.02243629498773
>100	0.08353189870236373	7.903163851491538
>500	0.016916960341056105	5.236186757610242
>1k	0.013445196090337673	10.737377620766738
>5k	2.5249194535845393E-4	0.597109505245073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	5401	0.14949466319829452	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	5352	0.14813838871269625	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	5333	0.1476124863611377	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	5333	0.1476124863611377	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	4967	0.13748194632585242	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	4826	0.13357919729586545	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4766	0.1319184530277859	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	4688	0.12975948547928248	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	4599	0.1272960481482978	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	4570	0.12649335508539272	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4310	0.1192967965903813	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	4260	0.11791284303364835	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	3748	0.1037411586127028	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	3681	0.10188666084668065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7679071134659236E-5	0.0
3	0.0	0.0	0.0	2.7679071134659236E-5	0.0
4	0.0	0.0	0.0	2.7679071134659236E-5	0.0
5	0.0	0.0	0.0	2.7679071134659236E-5	0.0
6	0.0	0.0	0.0	2.7679071134659236E-5	0.0
7	0.0	0.0	0.0	5.535814226931847E-5	0.0
8	0.0	0.0	0.0	5.535814226931847E-5	0.0
9	0.0	0.0	0.0	5.535814226931847E-5	0.0
10	0.0	0.0	0.0	5.535814226931847E-5	0.0
11	0.0	0.0	0.0	5.535814226931847E-5	0.0
12	0.0	0.0	0.0	5.535814226931847E-5	2.214325690772739E-4
13	0.0	0.0	0.0	5.535814226931847E-5	2.214325690772739E-4
14	0.0	0.0	0.0	1.1071628453863694E-4	2.214325690772739E-4
15	0.0	0.0	0.0	1.383953556732962E-4	3.321488536159108E-4
16	0.0	0.0	0.0	1.9375349794261464E-4	3.875069958852293E-4
17	0.0	0.0	0.0	2.767907113465924E-4	3.875069958852293E-4
18	0.0	0.0	0.0	4.151860670198885E-4	4.151860670198885E-4
19	0.0	0.0	0.0	5.535814226931847E-4	4.151860670198885E-4
20	0.0	0.0	0.0	8.30372134039777E-4	4.151860670198885E-4
21	0.0	0.0	0.0	0.0010518047031170508	4.70544209289207E-4
22	0.0	0.0	0.0	0.0013839535567329616	4.982232804238662E-4
23	0.0	0.0	0.0	0.0020482512639647833	4.982232804238662E-4
24	0.0	0.0	0.0	0.0033491676072937675	4.982232804238662E-4
25	0.0	5.535814226931847E-5	0.0	0.003930428101121612	4.982232804238662E-4
26	0.0	5.535814226931847E-5	0.0	0.005092949088777299	5.259023515585254E-4
27	0.0	5.535814226931847E-5	0.0	0.006393865432106283	5.259023515585254E-4
28	0.0	5.535814226931847E-5	0.0	0.010268935390958576	5.259023515585254E-4
29	0.0	5.535814226931847E-5	0.0	0.016856554321007473	5.259023515585254E-4
30	0.0	5.535814226931847E-5	0.0	0.028786233980045605	5.259023515585254E-4
31	0.0	5.535814226931847E-5	0.0	0.06568243580254636	5.535814226931847E-4
32	0.0	5.535814226931847E-5	0.0	0.10637067037049544	5.535814226931847E-4
33	0.0	5.535814226931847E-5	0.0	0.1484151794240428	5.535814226931847E-4
34	0.0	5.535814226931847E-5	0.0	0.19580174920657942	5.81260493827844E-4
35	0.0	5.535814226931847E-5	0.0	0.2506893472666087	5.81260493827844E-4
36	0.0	5.535814226931847E-5	0.0	0.324011206702321	5.81260493827844E-4
37	0.0	5.535814226931847E-5	0.0	0.44449820335149265	5.81260493827844E-4
38	0.0	5.535814226931847E-5	0.0	0.6128976721347594	6.089395649625032E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	785	0.0	21.299269	4
AGGACGT	935	0.0	17.411688	5
GTATCAA	8190	0.0	17.382542	1
TAACGGC	410	0.0	17.170656	36
TTAACGG	415	0.0	16.433664	35
TGTAGGA	1195	0.0	16.201214	2
CGCAATA	1800	0.0	16.133263	36
GGACGTG	955	0.0	16.125584	6
GTCCTAC	1170	0.0	15.797426	1
ATACGAA	1855	0.0	15.773516	40
GTATTAC	310	0.0	15.615428	1
TCTAGCG	1925	0.0	15.3142185	28
TACGACG	1480	0.0	15.310743	5
TATGCGT	115	8.22506E-5	15.30428	39
TAGCGGC	1890	0.0	15.24861	30
ATACCGT	1460	0.0	15.2191105	6
ACCGTCG	1405	0.0	15.188545	8
AACGAAT	320	0.0	15.124934	31
CGACGGT	1535	0.0	15.048794	7
TACGAAT	1960	0.0	15.040749	41
>>END_MODULE