FastQCFastQC Report
Thu 2 Feb 2017
SRR4062371_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062371_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070625
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT24920.23276123759486284No Hit
GTATCAACGCAGAGTACTTTTTTTT23420.21875072971395212No Hit
GTCCTACAGTGGACATTTCTAAATT21610.20184471687098657No Hit
CTTTAGGACGTGAAATATGGCGAGG20020.18699357851722126No Hit
CTGTAGGACGTGGAATATGGCAAGA19960.18643315820198483No Hit
GTACATGGAAGCAGTGGTATCAACG18780.17541155866900174No Hit
GTCCTACAGTGTGCATTTCTCATTT17310.16168126094570928No Hit
TCCATGTACTCTGCGTTGATACCAC16810.1570110916520724No Hit
TATCAACGCAGAGTACTTTTTTTTT15580.14552247518972564No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13250.12375948628137771No Hit
CTGTAGGACCTGGAATATGGCGAGA11930.1114302393461763No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10790.10078225335668417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT456.770493E-414.7747224
CTAGACA602.5717913E-514.2470534
GCGTTAT759.748637E-713.9226371
ATTCGAA551.9720566E-413.8075741
CGCCAGT1051.3606041E-913.57687818
GGCGGGT707.241426E-613.57433615
CGTCTTA801.9929357E-613.06529915
AATGTCC2400.013.0628558
GTCTTAC957.4525815E-812.9900221
TTAGGCA1102.750312E-912.9518674
TCGAACG604.1102635E-412.659313
GTTATTC1052.006891E-812.659313
GTATTAA604.1167534E-412.6569431
CGTTATT853.97196E-612.28525452
CGGGTCA701.08620734E-412.2191917
TTAGTAC550.00308074412.0838863
GTTTAGA550.003085062412.0816281
TATACAG951.039587E-611.9992045
ATGTCCA2700.011.9632869
CGGTTCT1209.986252E-911.87587712