##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062371_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070625
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80430683012259	32.0	32.0	32.0	32.0	32.0
2	30.291467133683597	32.0	32.0	32.0	21.0	32.0
3	30.381318388791595	32.0	32.0	32.0	21.0	32.0
4	30.15016649153532	32.0	32.0	32.0	21.0	32.0
5	30.21269632224168	32.0	32.0	32.0	21.0	32.0
6	33.476431056625806	36.0	36.0	36.0	21.0	36.0
7	33.469486514886164	36.0	36.0	36.0	21.0	36.0
8	33.296567892586104	36.0	36.0	36.0	21.0	36.0
9	33.63118458844133	36.0	36.0	36.0	27.0	36.0
10	33.10559626386456	36.0	32.0	36.0	21.0	36.0
11	33.75984401634559	36.0	36.0	36.0	32.0	36.0
12	33.36686514886165	36.0	36.0	36.0	21.0	36.0
13	33.56586293053123	36.0	36.0	36.0	27.0	36.0
14	33.328099007589024	36.0	36.0	36.0	21.0	36.0
15	33.20521938120257	36.0	36.0	36.0	21.0	36.0
16	33.34243549328663	36.0	36.0	36.0	21.0	36.0
17	33.20052305896089	36.0	36.0	36.0	21.0	36.0
18	33.29015388207822	36.0	36.0	36.0	21.0	36.0
19	33.230846468184474	36.0	36.0	36.0	21.0	36.0
20	33.12384635143024	36.0	32.0	36.0	21.0	36.0
21	33.09839953298307	36.0	32.0	36.0	21.0	36.0
22	33.024289550496206	36.0	32.0	36.0	21.0	36.0
23	32.999762755399885	36.0	32.0	36.0	21.0	36.0
24	33.01059007589025	36.0	32.0	36.0	21.0	36.0
25	32.13904401634559	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	41.0
5	100.0
6	193.0
7	64.0
8	147.0
9	170.0
10	119.0
11	47.0
12	66.0
13	62.0
14	719.0
15	1341.0
16	1769.0
17	2101.0
18	2359.0
19	2622.0
20	3218.0
21	3745.0
22	4818.0
23	6285.0
24	8389.0
25	11505.0
26	15909.0
27	21362.0
28	29465.0
29	40299.0
30	55375.0
31	78423.0
32	114199.0
33	159385.0
34	273628.0
35	232691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83335154842271	18.759300204289232	11.630568095944014	25.77678015134404
2	15.332703153576427	20.807047302712327	39.93203772858806	23.928211815123195
3	19.113066009581658	25.10333116318431	29.055462096961758	26.72814073027227
4	12.178566791128084	16.358191052790385	37.534318748896524	33.92892340718501
5	13.432119021212543	37.62757378326636	34.68745532640108	14.25285186912002
6	31.883682834910847	36.84161836315333	17.984045122941513	13.290653678994302
7	28.238214605496083	31.22458936412425	21.973014956174538	18.564181074205134
8	25.864947584316617	35.687536341353514	20.48511850786843	17.96239756646144
9	26.98780718106738	14.774794651688502	19.902378917884985	38.33501924935913
10	15.624111671329613	27.65039871931058	33.047696704020105	23.677792905339697
11	35.7923793636129	21.452817062086453	24.307002352694752	18.447801221605893
12	24.47386461091279	24.171318642162472	30.61886783277017	20.73594891415457
13	29.412265272209893	20.88963948167952	25.95436408301282	23.743731163097767
14	21.958709766710275	21.895307179922142	26.09465314202834	30.05132991133924
15	23.591609877245084	30.580448979286512	22.953523686986184	22.874417456482224
16	23.252097595094252	26.652198021579537	27.26364009541451	22.8320642879117
17	21.93367752964181	27.517718691056537	27.512481869844418	23.036121909457233
18	22.561322885366955	25.81251829469587	30.009754053824047	21.616404766113124
19	24.636949251453323	25.717216947605475	26.303223127511053	23.342610673430148
20	24.401850788586305	26.244129787110687	27.084895762225287	22.26912366207772
21	24.63015627937077	25.445640758512788	25.322756973467765	24.601445988648678
22	24.233628941059603	27.365430826201926	25.931978108628122	22.468962124110348
23	22.531443835062962	26.878119131169985	27.02907198599337	23.56136504777368
24	23.731254646883606	26.449851065919113	27.23253823888372	22.58635604831356
25	23.15313503883436	26.745067965977263	27.27972430175304	22.822072693435334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	153.0
2	432.5
3	712.0
4	712.0
5	712.0
6	1701.5
7	2691.0
8	2691.0
9	2691.0
10	2938.5
11	3186.0
12	3186.0
13	3186.0
14	3748.5
15	4311.0
16	4311.0
17	4311.0
18	7291.5
19	10272.0
20	10272.0
21	10272.0
22	16973.0
23	23674.0
24	23674.0
25	23674.0
26	36361.0
27	49048.0
28	49048.0
29	49048.0
30	63246.5
31	77445.0
32	77445.0
33	77445.0
34	85863.5
35	94282.0
36	94282.0
37	94282.0
38	98951.5
39	103621.0
40	103621.0
41	103621.0
42	110990.5
43	118360.0
44	118360.0
45	118360.0
46	129480.0
47	140600.0
48	140600.0
49	140600.0
50	133095.0
51	125590.0
52	125590.0
53	125590.0
54	111261.5
55	96933.0
56	96933.0
57	96933.0
58	87077.5
59	77222.0
60	77222.0
61	77222.0
62	67999.5
63	58777.0
64	58777.0
65	58777.0
66	48652.5
67	38528.0
68	38528.0
69	38528.0
70	29682.0
71	20836.0
72	20836.0
73	20836.0
74	16312.5
75	11789.0
76	11789.0
77	11789.0
78	9549.0
79	7309.0
80	7309.0
81	7309.0
82	5144.5
83	2980.0
84	2980.0
85	2980.0
86	2194.0
87	1408.0
88	1408.0
89	1408.0
90	917.5
91	427.0
92	427.0
93	427.0
94	247.5
95	68.0
96	68.0
97	68.0
98	235.5
99	403.0
100	403.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007845884413309983
2	0.0028955049620548747
3	0.00354932866316404
4	0.01363689433741973
5	0.03381202568593111
6	0.04502043199065966
7	0.06528896672504379
8	0.08471687098657327
9	0.09144191476941038
10	0.11217746643315821
11	0.11329830706363106
12	0.12758902510215997
13	0.11656742556917687
14	0.11843549328663164
15	0.10993578517221249
16	0.11077641564506714
17	0.11899591360186806
18	0.12385288966725043
19	0.12637478108581435
20	0.11610040863981319
21	0.12347927612375949
22	0.14272037361354348
23	0.13235259778166958
24	0.12702860478692352
25	0.12516053706946875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1070625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.8379146416553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21459566739944	44.53639064865399
2	12.076288569484563	13.244769635227843
3	3.1608310894891	5.200001564462809
4	1.1657310051233218	2.5570502941633504
5	0.574944929485653	1.5764390483393387
6	0.31368277700415803	1.032102560994685
7	0.2205731121077322	0.846703864580566
8	0.14311651854062815	0.6278569142033478
9	0.11482300050504617	0.566698851053502
>10	0.7779246935462366	8.475938194271974
>50	0.11492353529612605	4.453384648506565
>100	0.11303226119640238	12.130578573661012
>500	0.007319859916683684	2.652265838129968
>1k	0.002212980905043904	2.0998193637510734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	2492	0.23276123759486284	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2342	0.21875072971395212	No Hit
GTCCTACAGTGGACATTTCTAAATT	2161	0.20184471687098657	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2002	0.18699357851722126	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1996	0.18643315820198483	No Hit
GTACATGGAAGCAGTGGTATCAACG	1878	0.17541155866900174	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1731	0.16168126094570928	No Hit
TCCATGTACTCTGCGTTGATACCAC	1681	0.1570110916520724	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1558	0.14552247518972564	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1325	0.12375948628137771	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1193	0.1114302393461763	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1079	0.10078225335668417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	9.340338587273788E-5
13	0.0	0.0	0.0	0.0	9.340338587273788E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	45	6.770493E-4	14.774722	4
CTAGACA	60	2.5717913E-5	14.247053	4
GCGTTAT	75	9.748637E-7	13.922637	1
ATTCGAA	55	1.9720566E-4	13.807574	1
CGCCAGT	105	1.3606041E-9	13.576878	18
GGCGGGT	70	7.241426E-6	13.574336	15
CGTCTTA	80	1.9929357E-6	13.065299	15
AATGTCC	240	0.0	13.062855	8
GTCTTAC	95	7.4525815E-8	12.990022	1
TTAGGCA	110	2.750312E-9	12.951867	4
TCGAACG	60	4.1102635E-4	12.65931	3
GTTATTC	105	2.006891E-8	12.65931	3
GTATTAA	60	4.1167534E-4	12.656943	1
CGTTATT	85	3.97196E-6	12.2852545	2
CGGGTCA	70	1.08620734E-4	12.21919	17
TTAGTAC	55	0.003080744	12.083886	3
GTTTAGA	55	0.0030850624	12.081628	1
TATACAG	95	1.039587E-6	11.999204	5
ATGTCCA	270	0.0	11.963286	9
CGGTTCT	120	9.986252E-9	11.875877	12
>>END_MODULE