FastQCFastQC Report
Thu 2 Feb 2017
SRR4062371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070625
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA11620.10853473438412141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA250.002352672935.24
TACCGTC2150.021.488377
CGTCGTA2050.020.39024410
TGTAGGA18150.020.3636362
CTGTAGG17700.020.2598881
TAGGACC10500.020.1142864
ATACCGT2100.019.904766
ATTTCGT7850.019.61783442
GTCCTAA9200.019.6086961
TTAGGAC10500.019.2761923
GATTTCG7550.019.23178941
GACGTGA9750.018.9538447
TAGGACG17200.018.8023244
GTAGGAC19600.018.6326523
CCGTCGT2250.018.5777789
TAGGACA4500.018.5777784
AGGACGT17800.018.4157315
CCGGTCC3600.018.3333349
GTCCTAC15600.018.1923081
GTCGTAG2300.018.17391411
Produced by FastQC (version 0.11.5)