##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070625
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119205137186224	32.0	32.0	32.0	32.0	32.0
2	31.052378750729712	32.0	32.0	32.0	32.0	32.0
3	31.28627577349679	32.0	32.0	32.0	32.0	32.0
4	31.468478692352598	32.0	32.0	32.0	32.0	32.0
5	31.32339801517805	32.0	32.0	32.0	32.0	32.0
6	34.91949842381786	36.0	36.0	36.0	36.0	36.0
7	34.93434862813777	36.0	36.0	36.0	36.0	36.0
8	34.848356800934035	36.0	36.0	36.0	32.0	36.0
9	35.039476007005256	36.0	36.0	36.0	36.0	36.0
10	34.85660058377116	36.0	36.0	36.0	32.0	36.0
11	35.05515002918856	36.0	36.0	36.0	36.0	36.0
12	34.934062813777	36.0	36.0	36.0	32.0	36.0
13	34.99667483946293	36.0	36.0	36.0	36.0	36.0
14	34.95090437828371	36.0	36.0	36.0	32.0	36.0
15	34.89803058960887	36.0	36.0	36.0	32.0	36.0
16	34.922616228838294	36.0	36.0	36.0	32.0	36.0
17	34.90740268534734	36.0	36.0	36.0	32.0	36.0
18	34.923338237011095	36.0	36.0	36.0	32.0	36.0
19	34.90214500875657	36.0	36.0	36.0	32.0	36.0
20	34.87516263864565	36.0	36.0	36.0	32.0	36.0
21	34.868609457092816	36.0	36.0	36.0	32.0	36.0
22	34.85625685931115	36.0	36.0	36.0	32.0	36.0
23	34.808341856392296	36.0	36.0	36.0	32.0	36.0
24	34.78572422650321	36.0	36.0	36.0	32.0	36.0
25	34.768822883829536	36.0	36.0	36.0	32.0	36.0
26	34.72258914185639	36.0	36.0	36.0	32.0	36.0
27	34.69314279042615	36.0	36.0	36.0	32.0	36.0
28	34.66501436077058	36.0	36.0	36.0	32.0	36.0
29	34.64157104495038	36.0	36.0	36.0	32.0	36.0
30	34.616114886164624	36.0	36.0	36.0	32.0	36.0
31	34.620707530647984	36.0	36.0	36.0	32.0	36.0
32	34.57594302393462	36.0	36.0	36.0	32.0	36.0
33	34.56031850554582	36.0	36.0	36.0	32.0	36.0
34	34.5380389959136	36.0	36.0	36.0	32.0	36.0
35	34.500187273788676	36.0	36.0	36.0	32.0	36.0
36	34.45809223584355	36.0	36.0	36.0	32.0	36.0
37	34.442315469935785	36.0	36.0	36.0	32.0	36.0
38	34.3887887915937	36.0	36.0	36.0	32.0	36.0
39	34.34501903093987	36.0	36.0	36.0	32.0	36.0
40	34.346843199065965	36.0	36.0	36.0	32.0	36.0
41	34.30078038528897	36.0	36.0	36.0	32.0	36.0
42	34.27387180385289	36.0	36.0	36.0	32.0	36.0
43	34.261054991243434	36.0	36.0	36.0	32.0	36.0
44	34.202403269118506	36.0	36.0	36.0	32.0	36.0
45	34.160033625218915	36.0	36.0	36.0	32.0	36.0
46	34.12741903093987	36.0	36.0	36.0	32.0	36.0
47	34.10465755983655	36.0	36.0	36.0	32.0	36.0
48	34.05320537069469	36.0	36.0	36.0	32.0	36.0
49	34.023373263280796	36.0	36.0	36.0	32.0	36.0
50	33.43888849970811	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	19.0
22	66.0
23	240.0
24	681.0
25	1596.0
26	3490.0
27	6790.0
28	12161.0
29	19404.0
30	29112.0
31	43579.0
32	66584.0
33	111982.0
34	232558.0
35	542362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0153089344466	19.66602085407936	12.213517972210752	26.10515223926329
2	14.885894054086368	21.406540729172114	39.3984965215247	24.309068695216816
3	18.83805645327007	25.402383665539595	29.431731146438512	26.327828734751822
4	12.10013973159578	17.06771004573034	37.783386137430135	33.04876408524375
5	13.53555166374781	37.57664915353182	34.40569760653824	14.482101576182135
6	32.33976852729673	37.211511428392626	17.426302255789388	13.022417788521262
7	28.25826036193812	31.381202568593114	21.89748978400467	18.4630472854641
8	25.905896088733215	35.8800700525394	20.221179217746645	17.992854640980735
9	26.912691185055458	14.573543490951547	19.729503794512553	38.784261529480446
10	15.662346760070053	27.745382370110917	32.75161704611792	23.840653823701107
11	35.95283129013427	21.321004086398133	24.336252189141856	18.389912434325744
12	24.608709865732635	24.150005837711618	30.412702860478692	20.828581436077055
13	29.510799766491537	20.913485113835375	25.873858727378867	23.70185639229422
14	21.958388791593695	21.813333333333336	25.999112667834208	30.22916520723876
15	23.666176298890836	30.57597197898424	22.851231757151197	22.90661996497373
16	23.579217746643316	26.66134267367192	26.891955633391714	22.867483946293053
17	22.133053123175713	27.522148277875075	27.272107413893753	23.072691185055458
18	22.846374781085814	25.703304144775245	29.771395213076474	21.678925861062464
19	24.76114419147694	25.67406888499708	26.375901926444833	23.188884997081143
20	24.587974314068887	26.207495621716287	26.94267367192061	22.26185639229422
21	24.79187390542907	25.40516053706947	25.400116754232343	24.40284880326912
22	24.447122008172798	27.219988324576764	25.881891418563924	22.450998248686517
23	22.72177466433158	26.75689433741973	27.002638645650904	23.518692352597782
24	24.038108581436077	26.522078225335665	26.886258026853472	22.55355516637478
25	23.525697606538237	26.431757151196734	27.141436077057797	22.901109165207238
26	21.81529480443666	27.42528896672504	28.12086398131932	22.638552247518973
27	23.271079976649155	26.99843549328663	26.95687098657326	22.77361354349095
28	22.264845300642147	27.0122591943958	26.908114419147694	23.81478108581436
29	22.939871570344426	26.209643899591363	26.66246351430239	24.18802101576182
30	22.719813193228255	26.508628137769996	27.308067717454758	23.463490951546994
31	23.708768242848805	25.956614127262114	26.598015178050204	23.736602451838877
32	22.639299474605952	27.222043199065965	26.753064798598945	23.38559252772913
33	22.345732632807938	26.75754816112084	27.88165791009924	23.01506129597198
34	23.087542323409224	26.791546993578518	28.032784588441327	22.088126094570928
35	23.376999416228838	27.048593111500292	27.193274956217163	22.381132516053707
36	22.14342089900759	26.993485113835376	27.540922358435495	23.32217162872154
37	23.244273204903678	26.690858143607706	26.122778750729715	23.942089900758905
38	22.962661996497374	27.3020899007589	26.482755399883246	23.25249270286048
39	23.771255107997664	25.805861062463514	26.527215411558668	23.89566841798015
40	24.685113835376534	25.999673088149443	27.0780151780502	22.23719789842382
41	23.390262697022767	25.595890251021597	28.213706946876826	22.80014010507881
42	23.739591360186807	27.406795096322238	27.03887915936953	21.814734384121422
43	22.19638061879743	26.718785755983653	27.668791593695268	23.41604203152364
44	21.879929947460596	26.9235259778167	27.288546409807356	23.907997664915353
45	22.149025102159953	27.12210157618214	27.253426736719206	23.4754465849387
46	22.5901903936396	25.63701168664256	27.26624846818304	24.506549451534806
47	23.73529480443666	25.740478692352596	27.18290718038529	23.341319322825452
48	22.688709865732633	26.775481611208406	26.51058960887332	24.02521891418564
49	22.797991827203738	26.064401634559253	27.283876240513717	23.853730297723292
50	22.095318155283127	27.665055458260362	26.159953298307066	24.079673088149445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	25.5
2	34.0
3	91.0
4	148.0
5	150.5
6	153.0
7	165.5
8	178.0
9	250.0
10	322.0
11	463.0
12	604.0
13	984.0
14	1364.0
15	1954.5
16	2545.0
17	3242.0
18	3939.0
19	4480.0
20	5021.0
21	5804.5
22	6588.0
23	6990.5
24	7393.0
25	9344.5
26	11296.0
27	15365.0
28	19434.0
29	23233.0
30	27032.0
31	30298.0
32	33564.0
33	40404.5
34	47245.0
35	54551.0
36	61857.0
37	65595.0
38	69333.0
39	66843.0
40	64353.0
41	63445.5
42	62538.0
43	60154.0
44	57770.0
45	60289.0
46	62808.0
47	65203.0
48	67598.0
49	67168.0
50	66738.0
51	63181.0
52	59624.0
53	57588.0
54	55552.0
55	55347.0
56	55142.0
57	54048.5
58	52955.0
59	48053.5
60	43152.0
61	38363.5
62	33575.0
63	29397.5
64	25220.0
65	21807.5
66	18395.0
67	16381.0
68	14367.0
69	13120.5
70	11874.0
71	9370.5
72	6867.0
73	6137.5
74	5408.0
75	4121.0
76	2834.0
77	2451.5
78	2069.0
79	1784.5
80	1500.0
81	1212.5
82	925.0
83	773.5
84	622.0
85	507.0
86	392.0
87	281.5
88	171.0
89	120.0
90	69.0
91	46.0
92	23.0
93	17.5
94	12.0
95	7.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012983070636310568
2	0.0038295388207822533
3	4.670169293636894E-4
4	9.340338587273788E-5
5	0.0
6	1.8680677174547577E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.340338587273788E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1070625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.90944078079177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73536151077504	51.22099963928726
2	11.431570998026341	14.154443354674568
3	2.8320682780073643	5.2599529003336745
4	1.0268212030483024	2.542797058503209
5	0.4959169098614897	1.5350969281631572
6	0.2778525067786994	1.0321015988526259
7	0.1931890075136503	0.8372156394116389
8	0.1310205705726807	0.6489128203947939
9	0.10344353335121376	0.5763718171946544
>10	0.6041702253456881	7.450624974202122
>50	0.08704049300104284	3.8406267744017617
>100	0.07774845327955815	9.229153203338756
>500	0.003492605603898366	1.4643150637938738
>1k	3.0370483512159706E-4	0.20738822744795107
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1162	0.10853473438412141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.604203152364273E-4
13	0.0	0.0	0.0	0.0	6.538237011091652E-4
14	0.0	0.0	0.0	0.0	6.538237011091652E-4
15	0.0	0.0	0.0	0.0	7.472270869819031E-4
16	0.0	0.0	0.0	9.340338587273788E-5	7.472270869819031E-4
17	0.0	0.0	0.0	9.340338587273788E-5	7.472270869819031E-4
18	0.0	0.0	0.0	1.8680677174547577E-4	8.40630472854641E-4
19	0.0	0.0	0.0	2.8021015761821365E-4	8.40630472854641E-4
20	0.0	0.0	0.0	3.7361354349095153E-4	9.340338587273788E-4
21	0.0	0.0	0.0	3.7361354349095153E-4	9.340338587273788E-4
22	0.0	0.0	0.0	7.472270869819031E-4	9.340338587273788E-4
23	0.0	0.0	0.0	0.0015878575598365441	9.340338587273788E-4
24	0.0	0.0	0.0	0.0032691185055458262	9.340338587273788E-4
25	0.0	0.0	0.0	0.004016345592527729	9.340338587273788E-4
26	0.0	0.0	0.0	0.0053239929947460594	9.340338587273788E-4
27	0.0	0.0	0.0	0.007472270869819031	9.340338587273788E-4
28	0.0	0.0	0.0	0.011768826619964973	9.340338587273788E-4
29	0.0	0.0	0.0	0.023350846468184472	9.340338587273788E-4
30	0.0	0.0	0.0	0.04595446584938704	9.340338587273788E-4
31	0.0	0.0	0.0	0.09956800934033859	9.340338587273788E-4
32	0.0	0.0	0.0	0.16607122008172795	0.0010274372446001168
33	0.0	0.0	0.0	0.24079392877991826	0.0010274372446001168
34	0.0	0.0	0.0	0.321774664331582	0.0012142440163455926
35	0.0	0.0	0.0	0.4210624635143024	0.0012142440163455926
36	0.0	0.0	0.0	0.5513601868067718	0.0012142440163455926
37	0.0	0.0	0.0	0.757594862813777	0.0012142440163455926
38	0.0	0.0	0.0	1.0181903093987157	0.0013076474022183304
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCA	25	0.0023526729	35.2	4
TACCGTC	215	0.0	21.48837	7
CGTCGTA	205	0.0	20.390244	10
TGTAGGA	1815	0.0	20.363636	2
CTGTAGG	1770	0.0	20.259888	1
TAGGACC	1050	0.0	20.114286	4
ATACCGT	210	0.0	19.90476	6
ATTTCGT	785	0.0	19.617834	42
GTCCTAA	920	0.0	19.608696	1
TTAGGAC	1050	0.0	19.276192	3
GATTTCG	755	0.0	19.231789	41
GACGTGA	975	0.0	18.953844	7
TAGGACG	1720	0.0	18.802324	4
GTAGGAC	1960	0.0	18.632652	3
CCGTCGT	225	0.0	18.577778	9
TAGGACA	450	0.0	18.577778	4
AGGACGT	1780	0.0	18.415731	5
CCGGTCC	360	0.0	18.333334	9
GTCCTAC	1560	0.0	18.192308	1
GTCGTAG	230	0.0	18.173914	11
>>END_MODULE