FastQCFastQC Report
Thu 2 Feb 2017
SRR4062370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702202
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG36890.216719284785237No Hit
GTATCAACGCAGAGTACTTTTTTTT35990.211432015706714No Hit
TCCATGTACTCTGCGTTGATACCAC31310.18393821649839442No Hit
TATCAACGCAGAGTACTTTTTTTTT23580.13852644985730247No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22530.13235796926569232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC551.1348566E-515.5361451
TACACCG551.9609916E-413.8176265
AACCGCG1750.012.4866817
CGTCTTA1405.2750693E-1112.2159515
CGCAATC1103.8118742E-812.09148912
CGCATCG2300.011.97883613
CAACGGA1052.7175156E-711.762814514
CGATAAC1052.7185524E-711.76246810
AGCTCGC658.02087E-411.69286910
CGCAAGA2450.011.6267132
CGGTCCA3200.011.57868110
ATTTGCG2300.011.56747316
GTATTAC1157.133167E-811.5582921
TCTCGTC1657.2759576E-1211.51570412
TTGCGCG2400.011.48208218
AAGACGG2400.011.4787045
GAACCGC2000.011.4008836
TTTGCGC2450.011.24775317
CAAGACG2450.011.2431224
ACGGAAT2200.011.230123518
Produced by FastQC (version 0.11.5)