##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062370_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1702202
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146736991261907	32.0	32.0	32.0	32.0	32.0
2	30.80777369548385	32.0	32.0	32.0	32.0	32.0
3	30.799505581593724	32.0	32.0	32.0	32.0	32.0
4	30.873550260192385	32.0	32.0	32.0	32.0	32.0
5	30.66180335823833	32.0	32.0	32.0	32.0	32.0
6	34.29050782457076	36.0	36.0	36.0	32.0	36.0
7	34.17650137880228	36.0	36.0	36.0	32.0	36.0
8	34.17136215325796	36.0	36.0	36.0	32.0	36.0
9	34.33711627644663	36.0	36.0	36.0	32.0	36.0
10	34.01509045342445	36.0	36.0	36.0	32.0	36.0
11	34.321520007613664	36.0	36.0	36.0	32.0	36.0
12	34.109396534606354	36.0	36.0	36.0	32.0	36.0
13	34.23023589444731	36.0	36.0	36.0	32.0	36.0
14	34.136856847777175	36.0	36.0	36.0	32.0	36.0
15	34.07600273058074	36.0	36.0	36.0	32.0	36.0
16	34.07811235094307	36.0	36.0	36.0	32.0	36.0
17	33.9994524739132	36.0	36.0	36.0	32.0	36.0
18	34.013298069206826	36.0	36.0	36.0	32.0	36.0
19	34.004694507467384	36.0	36.0	36.0	32.0	36.0
20	33.983345689877	36.0	36.0	36.0	32.0	36.0
21	33.974409030185605	36.0	36.0	36.0	32.0	36.0
22	33.915719168465316	36.0	36.0	36.0	32.0	36.0
23	33.872510430606944	36.0	36.0	36.0	32.0	36.0
24	33.854371572821556	36.0	36.0	36.0	32.0	36.0
25	33.37327884704636	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	67.0
5	145.0
6	314.0
7	80.0
8	290.0
9	304.0
10	223.0
11	61.0
12	119.0
13	86.0
14	270.0
15	370.0
16	592.0
17	735.0
18	1110.0
19	1587.0
20	2273.0
21	3417.0
22	5193.0
23	7658.0
24	10465.0
25	14618.0
26	20392.0
27	26349.0
28	35679.0
29	47856.0
30	62229.0
31	85198.0
32	122683.0
33	175711.0
34	386360.0
35	689761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40874509635728	17.23179857712842	11.301012719360347	26.058443607153958
2	16.746066576074984	19.891160730913914	37.162330074500204	26.2004426185109
3	19.15197368923523	24.132007376696198	28.01223417661653	28.703784757452045
4	12.77741479843287	15.393275063397347	35.64382090337986	36.18548923478993
5	14.68084543710203	36.44847070853694	33.5973977509469	15.27328610341413
6	34.24661881987983	34.92023935540257	17.03987826517292	13.793263559544682
7	30.085924511530678	30.28721455496323	21.116818770766855	18.51004216273924
8	28.130529145672206	32.64792752405408	19.69509251478996	19.526450815483752
9	27.394386033828695	14.61938748927527	18.56597054176132	39.42025593513472
10	15.771899125064333	26.778501580766118	31.87747959708845	25.572119697081096
11	37.00598950322356	21.313408711395113	22.3150315179741	19.365570267407232
12	24.67654093814599	23.55824380877531	28.9371294648057	22.828085788272997
13	29.330341387666753	19.805719596263792	25.375973460696894	25.48796555537256
14	23.548822752972235	19.642037847897846	25.247284226278005	31.56185517285191
15	25.096250777828622	27.098490453734343	22.66630828715374	25.138950481283295
16	25.691663804339132	25.77347539888299	24.015614192753056	24.519246604024826
17	23.96474729246124	25.809555853059997	25.546978120875163	24.678718733603596
18	24.67276689991088	24.88924576527926	26.5654863274184	23.87250100739146
19	25.358881791223535	25.388588844383868	25.422178403501782	23.83035096089081
20	25.356626919891585	24.525438563469358	25.558321412437884	24.559613104201176
21	26.135081491974336	24.497359290602745	24.580186620327286	24.78737259709563
22	25.355419981889955	24.755340787204585	25.02487336793345	24.86436586297201
23	24.176275438314715	24.56921580774573	25.564127409399855	25.69038134453969
24	24.458891139362628	25.384186849847257	25.21694063553561	24.9399813752545
25	24.76665284260869	24.754828861103512	25.278201813598756	25.200316482689043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	255.0
1	255.0
2	623.0
3	991.0
4	991.0
5	991.0
6	2548.0
7	4105.0
8	4105.0
9	4105.0
10	4222.0
11	4339.0
12	4339.0
13	4339.0
14	4766.0
15	5193.0
16	5193.0
17	5193.0
18	8512.5
19	11832.0
20	11832.0
21	11832.0
22	18729.5
23	25627.0
24	25627.0
25	25627.0
26	37745.0
27	49863.0
28	49863.0
29	49863.0
30	62366.5
31	74870.0
32	74870.0
33	74870.0
34	90724.0
35	106578.0
36	106578.0
37	106578.0
38	121757.5
39	136937.0
40	136937.0
41	136937.0
42	156895.0
43	176853.0
44	176853.0
45	176853.0
46	203180.0
47	229507.0
48	229507.0
49	229507.0
50	228509.5
51	227512.0
52	227512.0
53	227512.0
54	212291.5
55	197071.0
56	197071.0
57	197071.0
58	179729.0
59	162387.0
60	162387.0
61	162387.0
62	142259.0
63	122131.0
64	122131.0
65	122131.0
66	100916.5
67	79702.0
68	79702.0
69	79702.0
70	60633.0
71	41564.0
72	41564.0
73	41564.0
74	31904.0
75	22244.0
76	22244.0
77	22244.0
78	17688.5
79	13133.0
80	13133.0
81	13133.0
82	9240.5
83	5348.0
84	5348.0
85	5348.0
86	3883.0
87	2418.0
88	2418.0
89	2418.0
90	1609.5
91	801.0
92	801.0
93	801.0
94	489.5
95	178.0
96	178.0
97	178.0
98	470.5
99	763.0
100	763.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00887086256507747
2	0.003466098618142853
3	0.00499353190749394
4	0.013981889340983033
5	0.0350722182208692
6	0.046939199930443036
7	0.06896948775762218
8	0.0913522601900362
9	0.09945940611043812
10	0.12201842084546959
11	0.12107846189817659
12	0.13488410893654218
13	0.12231215801649864
14	0.1253670245952008
15	0.11490998130656643
16	0.11479248643815482
17	0.1253670245952008
18	0.13318043334457366
19	0.13329792821298528
20	0.12372209643743809
21	0.13394414998924922
22	0.1523320968956681
23	0.14410745610685452
24	0.13588281531804097
25	0.13318043334457366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1702202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.27070802094255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25589207902344	43.80529270039039
2	12.642394049627697	13.97508140405348
3	3.616575603417165	5.996720826364028
4	1.4755422546520924	3.2621706051775625
5	0.7761264806077869	2.144853004849736
6	0.4643304105561138	1.5398322328254808
7	0.3003764197802704	1.162141217583598
8	0.22305486222720675	0.986272013024921
9	0.1703678856593235	0.8474718297979615
>10	0.9044375401256028	9.192584641465043
>50	0.08293887177917904	3.2180283624232837
>100	0.07826120934950638	9.014382476152
>500	0.007356714180509745	2.718870892459168
>1k	0.0023456190140755707	2.13629779343339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3689	0.216719284785237	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3599	0.211432015706714	No Hit
TCCATGTACTCTGCGTTGATACCAC	3131	0.18393821649839442	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2358	0.13852644985730247	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2253	0.13235796926569232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1749486841162212E-4
2	0.0	0.0	0.0	0.0	1.1749486841162212E-4
3	0.0	0.0	0.0	0.0	1.1749486841162212E-4
4	0.0	0.0	0.0	0.0	1.1749486841162212E-4
5	0.0	0.0	0.0	0.0	1.1749486841162212E-4
6	0.0	0.0	0.0	0.0	1.7624230261743318E-4
7	0.0	0.0	0.0	0.0	1.7624230261743318E-4
8	0.0	0.0	0.0	0.0	1.7624230261743318E-4
9	0.0	0.0	0.0	0.0	1.7624230261743318E-4
10	0.0	0.0	0.0	0.0	1.7624230261743318E-4
11	0.0	0.0	0.0	0.0	1.7624230261743318E-4
12	0.0	0.0	0.0	0.0	4.1123203944067743E-4
13	0.0	0.0	0.0	0.0	4.699794736464885E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATAC	55	1.1348566E-5	15.536145	1
TACACCG	55	1.9609916E-4	13.817626	5
AACCGCG	175	0.0	12.486681	7
CGTCTTA	140	5.2750693E-11	12.21595	15
CGCAATC	110	3.8118742E-8	12.091489	12
CGCATCG	230	0.0	11.978836	13
CAACGGA	105	2.7175156E-7	11.7628145	14
CGATAAC	105	2.7185524E-7	11.762468	10
AGCTCGC	65	8.02087E-4	11.692869	10
CGCAAGA	245	0.0	11.626713	2
CGGTCCA	320	0.0	11.578681	10
ATTTGCG	230	0.0	11.567473	16
GTATTAC	115	7.133167E-8	11.558292	1
TCTCGTC	165	7.2759576E-12	11.515704	12
TTGCGCG	240	0.0	11.482082	18
AAGACGG	240	0.0	11.478704	5
GAACCGC	200	0.0	11.400883	6
TTTGCGC	245	0.0	11.247753	17
CAAGACG	245	0.0	11.243122	4
ACGGAAT	220	0.0	11.2301235	18
>>END_MODULE