FastQCFastQC Report
Thu 2 Feb 2017
SRR4062370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702202
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA1057.748713E-820.9528982
ACCGTAT550.004484471419.9999078
TACCGTA550.004484471419.9999077
GTATTAT1504.656613E-1019.0699391
GTCCTAA3750.018.1897891
ATAGGGG1102.7979113E-617.9999163
GTATTAG4900.017.9622631
CGAATGT1206.4099077E-616.49992433
GTATAGC2301.8189894E-1216.2636591
ACGGGAT953.3474376E-416.2104543
CGTCTTA2750.015.99992615
GATATAC2353.6379788E-1215.9176241
CGCAATA3500.015.71421236
TTAACGG2252.5465852E-1115.64437235
GTATAGG1552.6638918E-715.6155821
CATGGGT14050.015.5017074
GAGCGAA4450.015.32577216
CGTCCAA1158.221007E-515.304275510
ACCGTCG3750.015.25326258
ACACGCT5050.015.2474529