##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1702202
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23154654970444	32.0	32.0	32.0	32.0	32.0
2	31.328079158642748	32.0	32.0	32.0	32.0	32.0
3	31.443267602787447	32.0	32.0	32.0	32.0	32.0
4	31.56392719547974	32.0	32.0	32.0	32.0	32.0
5	31.47617145321178	32.0	32.0	32.0	32.0	32.0
6	35.056165484472466	36.0	36.0	36.0	36.0	36.0
7	35.077278724851695	36.0	36.0	36.0	36.0	36.0
8	35.01103276814385	36.0	36.0	36.0	36.0	36.0
9	35.16673696776293	36.0	36.0	36.0	36.0	36.0
10	35.01284218911739	36.0	36.0	36.0	36.0	36.0
11	35.16393530262566	36.0	36.0	36.0	36.0	36.0
12	35.067371557547226	36.0	36.0	36.0	36.0	36.0
13	35.119945223892344	36.0	36.0	36.0	36.0	36.0
14	35.07983130086794	36.0	36.0	36.0	36.0	36.0
15	35.03242388388687	36.0	36.0	36.0	36.0	36.0
16	35.04747850137645	36.0	36.0	36.0	36.0	36.0
17	35.021669578581154	36.0	36.0	36.0	36.0	36.0
18	35.03954113554091	36.0	36.0	36.0	36.0	36.0
19	35.01894546005703	36.0	36.0	36.0	36.0	36.0
20	35.006042173608066	36.0	36.0	36.0	36.0	36.0
21	35.00353483311616	36.0	36.0	36.0	36.0	36.0
22	34.98336448905594	36.0	36.0	36.0	36.0	36.0
23	34.93914882017528	36.0	36.0	36.0	32.0	36.0
24	34.91427926885293	36.0	36.0	36.0	32.0	36.0
25	34.895306784976164	36.0	36.0	36.0	32.0	36.0
26	34.840298037483215	36.0	36.0	36.0	32.0	36.0
27	34.823337065753655	36.0	36.0	36.0	32.0	36.0
28	34.81289294690055	36.0	36.0	36.0	32.0	36.0
29	34.78147423161293	36.0	36.0	36.0	32.0	36.0
30	34.77137143535256	36.0	36.0	36.0	32.0	36.0
31	34.768110952754135	36.0	36.0	36.0	32.0	36.0
32	34.72697893669494	36.0	36.0	36.0	32.0	36.0
33	34.70485347802435	36.0	36.0	36.0	32.0	36.0
34	34.692676309862165	36.0	36.0	36.0	32.0	36.0
35	34.65741022510842	36.0	36.0	36.0	32.0	36.0
36	34.62540638537612	36.0	36.0	36.0	32.0	36.0
37	34.60523780373892	36.0	36.0	36.0	32.0	36.0
38	34.57122656417981	36.0	36.0	36.0	32.0	36.0
39	34.548560629114526	36.0	36.0	36.0	32.0	36.0
40	34.530178556951526	36.0	36.0	36.0	32.0	36.0
41	34.49997297618027	36.0	36.0	36.0	32.0	36.0
42	34.46876340175843	36.0	36.0	36.0	32.0	36.0
43	34.46409298073907	36.0	36.0	36.0	32.0	36.0
44	34.432467474483055	36.0	36.0	36.0	32.0	36.0
45	34.36724019828434	36.0	36.0	36.0	32.0	36.0
46	34.38791870765044	36.0	36.0	36.0	32.0	36.0
47	34.34851739100295	36.0	36.0	36.0	32.0	36.0
48	34.31605767118121	36.0	36.0	36.0	32.0	36.0
49	34.31052718772508	36.0	36.0	36.0	32.0	36.0
50	33.76847401189753	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	40.0
22	157.0
23	404.0
24	1170.0
25	2646.0
26	5871.0
27	10900.0
28	18393.0
29	27943.0
30	40920.0
31	58586.0
32	88433.0
33	142674.0
34	311890.0
35	992156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.904859496129724	17.88952175920975	11.806955097282758	26.398663647377766
2	16.141835905004065	20.485730869918736	36.78830598730066	26.584127237776535
3	18.53667592335299	24.49757459935507	28.368992235310568	28.596757241981376
4	12.47415109857831	15.969098813300434	35.87739396075667	35.679356127364585
5	14.477952675416903	36.64177342054586	33.433223553961284	15.44705035007596
6	34.457039293382955	35.184746753456565	16.76829035451285	13.58992359864763
7	30.154353008632345	30.370661061378147	21.007847482261212	18.467138447728296
8	28.181026693659156	32.80121865677516	19.54527136027334	19.472483289292338
9	27.397512163656252	14.403695918580755	18.387359432076806	39.81143248568619
10	15.67875023058368	26.877421128632207	31.853681290469638	25.590147350314474
11	37.254685401615085	21.15036875764451	22.26715748189698	19.327788358843428
12	24.653771996508052	23.52875863146677	28.944684590900494	22.872784781124682
13	29.527752875393166	19.640559698555165	25.38141771658123	25.45026970947044
14	23.614706127709873	19.540454070668464	25.120696603575837	31.724143198045823
15	25.182087672320915	27.141667087689946	22.501442249509754	25.17480299047939
16	25.798935731481926	25.739718317802467	23.884474345582955	24.57687160513265
17	24.101604862407637	25.76503846194517	25.430530571577286	24.702826104069906
18	24.821965900639288	24.783133846629248	26.463721696954885	23.931178555776576
19	25.487456835322718	25.327487571980296	25.28994796152278	23.89510763117421
20	25.439636423879186	24.416314867448165	25.492979094138065	24.651069614534585
21	26.303634938744054	24.445806079419484	24.46560396474684	24.78495501708963
22	25.44697985315491	24.683968177689838	24.958259947996773	24.910792021158475
23	24.252644515750774	24.438051418104315	25.549082893804613	25.760221172340298
24	24.656709368218344	25.187081204228406	25.167753298374695	24.98845612917856
25	24.92336397207852	24.651774583745052	25.28354449119435	25.14131695298208
26	24.410263881724966	25.077106007395127	25.73407856411871	24.778551546761197
27	25.245946133302628	25.04761479542381	24.968129516943346	24.738309554330215
28	24.484755628297933	24.609770168287902	25.69460028833241	25.21087391508176
29	24.50314357520435	25.07493235232951	25.630271847877044	24.791652224589093
30	24.74201064268518	24.940811960037646	25.730083738592718	24.587093658684456
31	24.84846099346611	24.847403539650408	25.089971695486202	25.214163771397285
32	24.5703506399358	25.030695534372537	25.031165513846183	25.36778831184548
33	24.295001415813164	24.75211520136858	25.499147574729676	25.453735808088584
34	24.747415406632115	24.935642185827533	25.827017005032303	24.489925402508046
35	25.49891258499285	24.79799694748332	25.372488106581947	24.330602360941885
36	24.64031883407492	25.13761586462711	25.31209574421837	24.909969557079595
37	25.40092186473756	24.966778325956614	24.984226313915737	24.648073495390086
38	24.524057661781622	24.985166272863033	25.27161876205057	25.21915730330478
39	24.85128087030799	24.857801835504834	25.130624919956624	25.160292374230554
40	25.328545025796	24.904623540566867	25.324961432309443	24.441870001327693
41	24.326783777718507	25.054840729831124	25.791827291943026	24.826548200507343
42	25.454910756772698	25.22979058889603	25.233080445211552	24.082218209119716
43	24.781958897945135	24.442809960274985	25.36978572460848	25.405445417171407
44	24.47964460152203	24.85803682524166	25.48980673269095	25.172511840545365
45	24.574345465461796	25.14666296949481	25.371724389937267	24.907267175106128
46	24.447420984608154	24.777405710257312	25.53389730936435	25.24127599577018
47	24.746299205382204	24.580807683224435	25.8653790795687	24.80751403182466
48	24.967894527206525	25.568587041960946	24.851104628005373	24.612413802827163
49	24.21469367325382	25.124750176536036	25.60818281261566	25.05237333759448
50	24.124810098918932	25.79529339056117	24.921719043920756	25.158177466599145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	69.0
2	70.0
3	196.0
4	322.0
5	365.5
6	409.0
7	352.5
8	296.0
9	383.5
10	471.0
11	689.0
12	907.0
13	1589.0
14	2271.0
15	2958.0
16	3645.0
17	4230.0
18	4815.0
19	5124.5
20	5434.0
21	6353.5
22	7273.0
23	8013.0
24	8753.0
25	10464.5
26	12176.0
27	14762.0
28	17348.0
29	20145.0
30	22942.0
31	25456.0
32	27970.0
33	32712.5
34	37455.0
35	43046.5
36	48638.0
37	56925.5
38	65213.0
39	69707.5
40	74202.0
41	81467.5
42	88733.0
43	90585.0
44	92437.0
45	102171.0
46	111905.0
47	119425.5
48	126946.0
49	129034.5
50	131123.0
51	126462.0
52	121801.0
53	119831.0
54	117861.0
55	118278.0
56	118695.0
57	114857.0
58	111019.0
59	101823.0
60	92627.0
61	82033.0
62	71439.0
63	62327.0
64	53215.0
65	45219.0
66	37223.0
67	32771.0
68	28319.0
69	25371.5
70	22424.0
71	17437.0
72	12450.0
73	10927.0
74	9404.0
75	6993.0
76	4582.0
77	4068.5
78	3555.0
79	2973.5
80	2392.0
81	1888.5
82	1385.0
83	1190.5
84	996.0
85	789.5
86	583.0
87	425.0
88	267.0
89	177.0
90	87.0
91	52.5
92	18.0
93	16.5
94	15.0
95	12.5
96	10.0
97	10.5
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01503934315668763
2	0.0038773306575835297
3	5.287269078522995E-4
4	1.1749486841162211E-4
5	0.0
6	4.1123203944067743E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1749486841162211E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1702202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.21084184806505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7863623362818	46.44431319589714
2	12.489014536384817	14.53992100031364
3	3.4757859079878806	6.069852713628471
4	1.4296919275685196	3.328942827485855
5	0.7320459888992157	2.130650664266131
6	0.43114229622143235	1.5058293611614448
7	0.3104407723483381	1.264971309165219
8	0.20972175274476035	0.9766463824899398
9	0.160630644358106	0.8415400531214241
>10	0.826903260872561	8.756932038772192
>50	0.07493173382091459	3.019625650988642
>100	0.06633100674713421	7.96369492025303
>500	0.005679402939362142	2.2065377206408336
>1k	0.0013184328252090686	0.9505421618160868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.874743420581106E-5
6	0.0	0.0	0.0	0.0	5.874743420581106E-5
7	0.0	0.0	0.0	0.0	5.874743420581106E-5
8	0.0	0.0	0.0	0.0	5.874743420581106E-5
9	0.0	0.0	0.0	0.0	5.874743420581106E-5
10	0.0	0.0	0.0	0.0	1.1749486841162212E-4
11	0.0	0.0	0.0	0.0	1.1749486841162212E-4
12	0.0	0.0	0.0	0.0	3.5248460523486637E-4
13	0.0	0.0	0.0	0.0	3.5248460523486637E-4
14	0.0	0.0	0.0	0.0	3.5248460523486637E-4
15	0.0	0.0	0.0	0.0	4.1123203944067743E-4
16	0.0	0.0	0.0	5.874743420581106E-5	5.287269078522995E-4
17	0.0	0.0	0.0	5.874743420581106E-5	5.287269078522995E-4
18	0.0	0.0	0.0	1.1749486841162212E-4	5.874743420581106E-4
19	0.0	0.0	0.0	1.7624230261743318E-4	5.874743420581106E-4
20	0.0	0.0	0.0	2.937371710290553E-4	7.637166446755437E-4
21	0.0	0.0	0.0	5.287269078522995E-4	8.224640788813549E-4
22	0.0	0.0	0.0	7.637166446755437E-4	8.224640788813549E-4
23	0.0	0.0	0.0	0.001997412762997576	8.812115130871659E-4
24	0.0	0.0	0.0	0.004229815262818396	8.812115130871659E-4
25	0.0	0.0	0.0	0.005111026775905562	8.812115130871659E-4
26	0.0	0.0	0.0	0.0061684805916101615	8.812115130871659E-4
27	0.0	0.0	0.0	0.008107145920401926	9.39958947292977E-4
28	0.0	0.0	0.0	0.013511909867336544	9.39958947292977E-4
29	0.0	0.0	0.0	0.020855339143062927	9.39958947292977E-4
30	0.0	0.0	0.0	0.0366583989444261	9.39958947292977E-4
31	0.0	0.0	0.0	0.07231809150735341	9.39958947292977E-4
32	0.0	0.0	0.0	0.11614367742488847	9.39958947292977E-4
33	0.0	0.0	0.0	0.15844183005307244	9.39958947292977E-4
34	0.0	0.0	0.0	0.2082009068253944	9.39958947292977E-4
35	0.0	0.0	0.0	0.2662433718207357	9.39958947292977E-4
36	0.0	0.0	0.0	0.34660986181428527	9.39958947292977E-4
37	0.0	0.0	0.0	0.4699207262122827	9.39958947292977E-4
38	0.0	0.0	0.0	0.6279513242259145	9.39958947292977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGTA	105	7.748713E-8	20.952898	2
ACCGTAT	55	0.0044844714	19.999907	8
TACCGTA	55	0.0044844714	19.999907	7
GTATTAT	150	4.656613E-10	19.069939	1
GTCCTAA	375	0.0	18.189789	1
ATAGGGG	110	2.7979113E-6	17.999916	3
GTATTAG	490	0.0	17.962263	1
CGAATGT	120	6.4099077E-6	16.499924	33
GTATAGC	230	1.8189894E-12	16.263659	1
ACGGGAT	95	3.3474376E-4	16.21045	43
CGTCTTA	275	0.0	15.999926	15
GATATAC	235	3.6379788E-12	15.917624	1
CGCAATA	350	0.0	15.714212	36
TTAACGG	225	2.5465852E-11	15.644372	35
GTATAGG	155	2.6638918E-7	15.615582	1
CATGGGT	1405	0.0	15.501707	4
GAGCGAA	445	0.0	15.325772	16
CGTCCAA	115	8.221007E-5	15.3042755	10
ACCGTCG	375	0.0	15.2532625	8
ACACGCT	505	0.0	15.247452	9
>>END_MODULE