FastQCFastQC Report
Thu 2 Feb 2017
SRR4062369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177860
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG81530.25655629889296566No Hit
TCCATGTACTCTGCGTTGATACCAC69900.21995934370928863No Hit
GTATCAACGCAGAGTACTTTTTTTT45110.14195087259979985No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG33500.1054168528506605No Hit
GAATAGGACCGCGGTTCTATTTTGT33130.1042525473117129No Hit
GTCTTGCGCCGGTCCAAGAATTTCA32690.10286796775188334No Hit
GAGTACATGGAAGCAGTGGTATCAA32310.10167219449566689No Hit
GCGCAAGACGGACCAGAGCGAAAGC32280.10157779134386034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT3250.012.2692921
CGTTATT3350.011.6214692
CGGTCCA7700.011.35127910
CGACCAT5200.011.32755310
CGGACCA7600.011.2506259
ATACCGT6450.011.1942436
CGATAAC2400.011.08394910
CCGTCGT6200.010.8796379
AATCGTA1053.4663608E-610.85791813
AACCGTC700.001491452810.8577467
CGTCGTA6300.010.85774610
AATTCCG2650.010.7542975
ACCGTCG6450.010.7525358
TAACGAA2300.010.73989713
AAGACGG8150.010.7235085
ACGGTAT4700.010.7133629
CGAGCCG7650.010.681005515
CCGACCA5700.010.6672599
AGGCCCG7900.010.58286710
GGTCCAA9000.010.55547711
Produced by FastQC (version 0.11.5)