##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062369_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3177860
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15522017961773	32.0	32.0	32.0	32.0	32.0
2	30.734360229840206	32.0	32.0	32.0	32.0	32.0
3	30.71091552176622	32.0	32.0	32.0	32.0	32.0
4	30.800983051487478	32.0	32.0	32.0	32.0	32.0
5	30.547729604199052	32.0	32.0	32.0	32.0	32.0
6	34.228188466452266	36.0	36.0	36.0	32.0	36.0
7	34.11082111861441	36.0	36.0	36.0	32.0	36.0
8	34.105440453638614	36.0	36.0	36.0	32.0	36.0
9	34.29228757717458	36.0	36.0	36.0	32.0	36.0
10	33.88740599019466	36.0	36.0	36.0	32.0	36.0
11	34.26511205654119	36.0	36.0	36.0	32.0	36.0
12	34.00439037591335	36.0	36.0	36.0	32.0	36.0
13	34.1387754652502	36.0	36.0	36.0	32.0	36.0
14	34.02823315061079	36.0	36.0	36.0	32.0	36.0
15	33.95600435513207	36.0	36.0	36.0	32.0	36.0
16	33.9578751109237	36.0	36.0	36.0	32.0	36.0
17	33.86814711787178	36.0	36.0	36.0	32.0	36.0
18	33.879623394359726	36.0	36.0	36.0	32.0	36.0
19	33.879610492595646	36.0	36.0	36.0	32.0	36.0
20	33.87825423398136	36.0	36.0	36.0	32.0	36.0
21	33.85788643930192	36.0	36.0	36.0	32.0	36.0
22	33.801872014500326	36.0	36.0	36.0	32.0	36.0
23	33.749726230859764	36.0	36.0	36.0	32.0	36.0
24	33.71823711554316	36.0	36.0	36.0	27.0	36.0
25	33.25131975606226	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	22.0
4	106.0
5	290.0
6	525.0
7	153.0
8	477.0
9	560.0
10	391.0
11	135.0
12	197.0
13	172.0
14	503.0
15	641.0
16	1026.0
17	1371.0
18	1923.0
19	2748.0
20	4437.0
21	6883.0
22	10362.0
23	15753.0
24	22333.0
25	31524.0
26	42944.0
27	55090.0
28	73603.0
29	96110.0
30	121766.0
31	163027.0
32	230787.0
33	329089.0
34	709052.0
35	1253856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56307521883689	16.890415550125173	11.301912295305097	26.244596935732844
2	17.15063083962893	19.515071832293867	36.41209548016536	26.922201847911843
3	19.27033572625301	23.427482953122187	27.618900163262328	29.68328115736248
4	13.144212606132172	15.125734640143564	34.570778805100765	37.1592739486235
5	15.430128444849048	35.67769421286766	32.9156137110249	15.976563631258397
6	35.34912567075983	33.90920953836613	16.652725461090814	14.088939329783232
7	31.069001815944986	29.62449582886852	20.66458485331214	18.641917501874357
8	28.61845959255559	32.10136474097889	19.376789702901444	19.903385963564073
9	27.31640618478963	14.861436282549473	18.379209410546295	39.4429481221146
10	16.371190027399575	26.186771396445714	30.748137210208633	26.693901365946083
11	37.623330973595195	20.998802488027245	21.69752441730378	19.680342121073785
12	25.18786876630165	23.203628001882944	27.935652308714147	23.672850923101258
13	29.6315152576772	19.1017190031716	24.8921035113183	26.3746622278329
14	24.165252053237353	19.298981856401298	24.32583745745133	32.20992863291002
15	25.795146065688368	26.441558130618777	21.977506158068053	25.785789645624803
16	26.66800867834217	25.18625137390808	23.074725163254463	25.071014784495294
17	24.822750449267435	25.26464561431808	24.816417857781968	25.096186078632517
18	25.82873998753542	24.098318920509648	25.60550558731915	24.467435504635777
19	25.89060881269803	25.08759260239512	24.624738167436725	24.397060417470122
20	25.944086810146754	23.842797593899874	24.678341790851512	25.534773805101857
21	26.87450276717452	24.158402427362656	23.7717384283583	25.195356377104527
22	25.982054674249355	24.100102294407463	24.18528460598375	25.732558425359432
23	24.627140098143666	23.878978204266506	24.843020868684558	26.65086082890527
24	24.98352860068255	24.883770685352	24.449921431976666	25.682779281988783
25	25.460308558051164	24.043928483093584	24.37791350733113	26.117849451524123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	347.0
1	347.0
2	875.5
3	1404.0
4	1404.0
5	1404.0
6	3221.0
7	5038.0
8	5038.0
9	5038.0
10	5280.5
11	5523.0
12	5523.0
13	5523.0
14	6593.0
15	7663.0
16	7663.0
17	7663.0
18	12291.0
19	16919.0
20	16919.0
21	16919.0
22	27372.0
23	37825.0
24	37825.0
25	37825.0
26	56757.5
27	75690.0
28	75690.0
29	75690.0
30	94001.0
31	112312.0
32	112312.0
33	112312.0
34	144971.0
35	177630.0
36	177630.0
37	177630.0
38	206908.5
39	236187.0
40	236187.0
41	236187.0
42	277132.5
43	318078.0
44	318078.0
45	318078.0
46	374954.0
47	431830.0
48	431830.0
49	431830.0
50	432562.5
51	433295.0
52	433295.0
53	433295.0
54	407755.5
55	382216.0
56	382216.0
57	382216.0
58	355448.5
59	328681.0
60	328681.0
61	328681.0
62	291104.5
63	253528.0
64	253528.0
65	253528.0
66	209721.5
67	165915.0
68	165915.0
69	165915.0
70	126415.0
71	86915.0
72	86915.0
73	86915.0
74	67491.0
75	48067.0
76	48067.0
77	48067.0
78	39447.0
79	30827.0
80	30827.0
81	30827.0
82	21638.5
83	12450.0
84	12450.0
85	12450.0
86	9073.0
87	5696.0
88	5696.0
89	5696.0
90	3896.0
91	2096.0
92	2096.0
93	2096.0
94	1245.5
95	395.0
96	395.0
97	395.0
98	864.0
99	1333.0
100	1333.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008338945076246279
2	0.003492916616842781
3	0.00478309302486579
4	0.014286343640059663
5	0.03423687638851303
6	0.04484149710811679
7	0.06617660941639972
8	0.0865991579238859
9	0.09563039278004695
10	0.117154311391943
11	0.11891650355899883
12	0.1290805762368386
13	0.11809834291000862
14	0.12108777605054975
15	0.11230828293253951
16	0.11079783250363452
17	0.11970319649072017
18	0.12794773841515988
19	0.12826241558784843
20	0.11835008464815945
21	0.12728691635251396
22	0.14682836877647223
23	0.13685310240224555
24	0.13144065503200267
25	0.12791627069789102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3177860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.279744616912254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75063173797764	37.53780644113152
2	12.62544997411916	12.19107000848149
3	3.890175203839494	5.63449996069246
4	1.721089535854497	3.3237505301558063
5	0.9586444443922667	2.3141554476840187
6	0.6196334221967802	1.7949446027858351
7	0.43598376068009886	1.4734429235929314
8	0.3152285969153212	1.2175324924015423
9	0.23990071643834351	1.0424110790751757
>10	1.2602826235147846	10.834006658081957
>50	0.0850035685961911	2.835002961842458
>100	0.07519343146803106	7.763173172285278
>500	0.014599440901442638	4.861113015853425
>1k	0.008052606416209597	6.698451523671933
>5k	1.3093668969446499E-4	0.4786391822641435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8153	0.25655629889296566	No Hit
TCCATGTACTCTGCGTTGATACCAC	6990	0.21995934370928863	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4511	0.14195087259979985	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3350	0.1054168528506605	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3313	0.1042525473117129	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3269	0.10286796775188334	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3231	0.10167219449566689	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3228	0.10157779134386034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1467717268853885E-5
2	0.0	0.0	0.0	0.0	3.1467717268853885E-5
3	0.0	0.0	0.0	0.0	3.1467717268853885E-5
4	0.0	0.0	0.0	0.0	3.1467717268853885E-5
5	0.0	0.0	0.0	0.0	3.1467717268853885E-5
6	0.0	0.0	0.0	0.0	3.1467717268853885E-5
7	0.0	0.0	0.0	0.0	3.1467717268853885E-5
8	0.0	0.0	0.0	0.0	6.293543453770777E-5
9	0.0	0.0	0.0	3.1467717268853885E-5	6.293543453770777E-5
10	3.1467717268853885E-5	0.0	0.0	3.1467717268853885E-5	9.440315180656165E-5
11	3.1467717268853885E-5	0.0	0.0	3.1467717268853885E-5	9.440315180656165E-5
12	6.293543453770777E-5	0.0	0.0	3.1467717268853885E-5	4.090803244951005E-4
13	6.293543453770777E-5	3.1467717268853885E-5	0.0	3.1467717268853885E-5	5.34951193570516E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	325	0.0	12.269292	1
CGTTATT	335	0.0	11.621469	2
CGGTCCA	770	0.0	11.351279	10
CGACCAT	520	0.0	11.327553	10
CGGACCA	760	0.0	11.250625	9
ATACCGT	645	0.0	11.194243	6
CGATAAC	240	0.0	11.083949	10
CCGTCGT	620	0.0	10.879637	9
AATCGTA	105	3.4663608E-6	10.857918	13
AACCGTC	70	0.0014914528	10.857746	7
CGTCGTA	630	0.0	10.857746	10
AATTCCG	265	0.0	10.754297	5
ACCGTCG	645	0.0	10.752535	8
TAACGAA	230	0.0	10.739897	13
AAGACGG	815	0.0	10.723508	5
ACGGTAT	470	0.0	10.713362	9
CGAGCCG	765	0.0	10.6810055	15
CCGACCA	570	0.0	10.667259	9
AGGCCCG	790	0.0	10.582867	10
GGTCCAA	900	0.0	10.555477	11
>>END_MODULE