FastQCFastQC Report
Thu 2 Feb 2017
SRR4062369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177860
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35100.11045168761367713No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC34730.10928738207472953No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT32410.10198687166835542No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA32380.10189246851654886No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31890.10035055037037503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1152.0238076E-719.1305855
ACCGTCG8900.016.8088578
ATACCGT9200.016.5001286
CGTCGTA8650.016.02299510
CGACGAA2201.8189894E-1115.99987432
TACCGTC9250.015.6971727
CGAGCCG11050.015.5292915
GTATAGG3900.015.2342451
CGGTCCA12250.015.08559510
CCGTCGT9850.014.964359
GTATAGA2950.014.9186571
TCTAGCG11600.014.41367928
CTAGCGG11450.014.41036729
CGCAATA11450.014.02609136
GTATATG3300.014.0031951
CGTCTTA3150.013.96814415
GTCGTAG10100.013.94048411
GGCGTTA8850.013.920794542
GTACTAA1606.335018E-613.7531381
TTATACT5450.013.7248794
Produced by FastQC (version 0.11.5)