FastQCFastQC Report
Thu 2 Feb 2017
SRR4062368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928485
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG153560.7962727218516088No Hit
TCCATGTACTCTGCGTTGATACCAC138680.7191137084291555No Hit
GAGTACATGGAAGCAGTGGTATCAA55520.28789438341496043No Hit
CATGTACTCTGCGTTGATACCACTG50230.26046352447646726No Hit
GTATCAACGCAGAGTACTTTTTTTT43060.2232840805087932No Hit
CCCATGTACTCTGCGTTGATACCAC40180.208350077910899No Hit
GCTTCCATGTACTCTGCGTTGATAC39430.20446101473436404No Hit
GCGTTGATACCACTGCTTCCATGTA38720.2007793682605776No Hit
CATGGAAGCAGTGGTATCAACGCAG34290.17780796843117783No Hit
GTACATGGGAAGCAGTGGTATCAAC32540.16873348768592963No Hit
GTATCAACGCAGAGTACATGGAAGC32040.166140778901573No Hit
TATCAACGCAGAGTACTTTTTTTTT31620.16396290352271342No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31410.16287396583328362No Hit
GAATAGGACCGCGGTTCTATTTTGT23810.12346479231106283No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23540.12206472956751024No Hit
GCGCAAGACGGACCAGAGCGAAAGC23420.12144247945926466No Hit
CAGTGGTATCAACGCAGAGTACATG22590.11713858287723264No Hit
CTATTGGAGCTGGAATTACCGCGGC22450.11641262441761278No Hit
GTATCTGATCGTCTTCGAACCTCCG22200.11511627002543448No Hit
GTCTTGCGCCGGTCCAAGAATTTCA22130.11475329079562455No Hit
GTCCTATTCCATTATTCCTAGCTGC21960.11387176980894329No Hit
ACTCTGCGTTGATACCACTGCTTCC21860.11335322805207196No Hit
ACGCAGAGTACATGGAAGCAGTGGT21760.11283468629520065No Hit
GATTAAGAGGGACGGCCGGGGGCAT21160.10972343575397268No Hit
GAACTACGACGGTATCTGATCGTCT21020.10899747729435283No Hit
TATCAACGCAGAGTACATGGAAGCA19720.10225643445502558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG400.00527349214.25253219
ATCGCCA3700.012.83978516
CGCATCG3700.012.83845113
TCAATAC905.421116E-712.6639853
GTCTAGT751.4873916E-512.6597141
TCGCCAG4000.012.58973717
ATCGTTG1001.4407487E-712.35155214
TCGCGTA2000.012.3502719
TGCACCG701.09173314E-412.2136015
CGCCAGT4050.012.20033118
CGGTTCT4550.012.11078412
CGGTCGA550.003068402412.09117310
GGTCGAT550.00306899912.09085911
TCTATTC550.003073775212.0883493
ACGGTAT4050.011.9632249
CATCGCC4250.011.84854815
GCATCGC4050.011.72986914
GGTTCTA4900.011.63320913
CGGACAT2450.011.6320015
TCCATTA3600.011.6116668
Produced by FastQC (version 0.11.5)