FastQCFastQC Report
Thu 2 Feb 2017
SRR4062368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928485
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA91710.4755546452266935No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC68420.35478627005136154No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC26920.1395914409497611No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA26380.13679131546265594No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24410.1265760428522908No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23360.12113135440514186No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23250.1205609584725834No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA21900.1135606447548205No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21890.11350879057913335No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG21620.11210872783558078No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20960.10868635224023002No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20810.10790853960492303No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC20450.10604178928018625No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20220.1048491432393822No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19460.10090822588716014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA500.002581042521.99992436
TGGTATA5850.020.30762144
TCGTATA901.0024967E-519.55548744
GAGTAAT1252.2861059E-819.3629441
GATATAC4700.019.1944071
GAGCGAA9050.018.47507316
AGAGCGA9250.018.07561115
TAGCGGC7250.017.90338530
CGCACGA750.00129127917.59993710
AGCGAAA9800.017.28565217
CAGAGCG10100.017.2078614
CGCAATA7450.017.12745736
CTAGCGG7600.017.07888829
AAAGCAT10150.016.68959621
GCGAAAG10050.016.63675718
CAATACG7550.016.60921338
ACCTTAC1206.401251E-616.502511
ACTATAC1206.410726E-616.499943
TCTAGCG8000.016.22494328
GCCGGTC9300.016.0859648
Produced by FastQC (version 0.11.5)